Skip to main content
SpringerLink
Log in

Phylogenetic relationships of Echinostoma Rudolphi, 1809 (Digenea: Echinostomatidae) and related genera re-assessed via DNA and morphological analyses

  • Published:
Systematic Parasitology Aims and scope Submit manuscript

Abstract

In order to investigate the relationships within the Echinostomatidae two data sets of gene sequences were analysed. The first consisted of all previously published ND1 sequences (20) together with 17 new sequences. The latter represented six species from the cosmopolitan genera Echinostoma, Echinoparyphium, Hypoderaeum and Isthmiophora. The second data-set of ITS sequences again included all previously published sequences (12) and three new sequences from species of Echinostoma, Echinoparyphium and Isthmiophora. All new isolates, as well as voucher material from five previously sequenced isolates, were identified on the basis of morphological characters. The phylogenetic trees inferred from the ND1 data set helped to clarify the generic affiliation of all isolates and confirmed the morphological identifications. The only exception was Echinoparyphium aconiatum, whose current position in the genus Echinoparyphium was not supported by the sequence data. Although the ITS data provided insufficient resolution for an unequivocal solution to the relationships within the genus Echinostoma, it supported the identification of Echinoparyphium ellisi and the distinct species status of three isolates of Echinostoma revolutum as predicted from the ND1 data.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Asada, J. (1926) On a new echinostomatid trematode and its life history. Transactions of the Japanese Pathology Society, 16, 293–294 (In Japanese).

    Google Scholar 

  • Barsiene, J. (1993) The karyotypes of trematodes. Vil'nyus: Academia, 370 pp (In Russian).

    Google Scholar 

  • Christensen, N.Ø., Fried, B. & Kanev, I. (1990) Taxonomy of 37-collar spined Echinostoma (Trematoda: Echinostomatidae) in studies on the population regulation in experimental rodent hosts. Angewandte Parasitologie, 31, 127–130.

    Google Scholar 

  • Dimitrov, V., Kanev, I., Busta, J. Le, N.T. & Ngo, H.Z. (1985) Argentophilic structures of Echinostoma audyi Lie et Umathevy, 1965 (Trematoda: Echinostomatidae) rediae and cercariae on materials from Czechoslovakia and Vietnam. Khelmintologiya, 19, 34–43 (In Bulgarian).

    Google Scholar 

  • Fried, B. & Graczyk, T.K. (Eds) (2000) Echinostomes as experimental models for biological research. Dordrecht: Kluwer Academic Publishers, 273 pp.

    Google Scholar 

  • Grabda-Kazubska, B. & Kiseliene, V. (1990) On the chaetotaxy and the excretory system of the cercaria of Hypoderaeum conoideum (Bloch, 1782) (Digenea, Echinostomatidae) and their taxonomic consequences. Acta Parasitologica Polonica, 35, 1–9.

    Google Scholar 

  • Grabda-Kazubska, B., Borsuk, P., Laskowski, Z. & Moné, H. (1998) A phylogenetic analysis of trematodes of the genus Echinoparyphium and related genera based on sequencing of internal transcribed spacer region of rDNA. Acta Parasitologica, 43, 116–121.

    Google Scholar 

  • Kanev, I. (1985) [On the morphology, biology, ecology and taxonomy of Echinostoma revolutum group (Trematoda: Echinostomatidae: Echinostoma)]. Thesis, Bulgarian Academy of Sciences, Sofia, 467 pp. (In Bulgarian).

    Google Scholar 

  • Kanev, I. (1994) Life-cycle, delimitation and redescription of Echinostoma revolutum (Frölich, 1802) (Trematoda: Echinostomatidae). Systematic Parasitology, 28, 125–144.

    Google Scholar 

  • Kanev, I., Dimitrov, V., Radev, V. & Fried, B. (1995a) Redescription of Echinostoma trivolvis (Cort, 1914) with a discussion of its identity. Systematic Parasitology, 32, 61–70.

    Google Scholar 

  • Kanev, I., Fried, B. Dimitrov, V. & Radev, V. (1995b) Redescription of Echinostoma jurini (Skvortzov, 1924) with a discussion of its identity and characteristics. Annalen des Naturhistorischen Museums in Wien Serie B Botanik und Zoologie, 97B, 37–53.

    Google Scholar 

  • Kanev, I., Vassilev, I., Bayssade-Dufour, C., Albaret, J.L. & Casone, J. (1987) Chétotaxie cercarienne d'Echinostoma revolutum (Frölich, 1802) et E. echinatum (Zeder, 1803) (Trematoda, Echinostomatidae). Annales de Parasitologie Humaine et Comparée, 62, 222–234.

    Google Scholar 

  • Kostadinova, A. (1995) Echinostoma echinatum (Zeder, 1803) sensu Kanev (Digenea: Echinostomatidae): a note of caution. Systematic Parasitology, 32, 23–26.

    Google Scholar 

  • Kostadinova, A. (1999) Cercarial chaetotaxy of Echinostoma miyagawai Ishii, 1932 (Digenea: Echinostomatidae), with a review of the sensory patterns in the 'revolutum' group. Systematic Parasitology, 44, 201–209.

    Google Scholar 

  • Kostadinova, A. & Gibson, D.I. (2000) The systematics of the echinostomes. In: Fried, B. & Graczyk, T.K. (Eds) Echinostomes as experimental models for biological research. Dordrecht: Kluwer Academic Publishers, pp. 31–57.

    Google Scholar 

  • Kostadinova, A. & Gibson, D.I. (2002) Isthmiophora Lühe, 1909 and Euparyphium Dietz, 1909 (Digenea: Echinostomatidae) redefined, with comments on their nominal species. Systematic Parasitology, 52, 205–217.

    Google Scholar 

  • Kostadinova, A., Gibson, D.I., Biserkov, V. & Chipev, N. (2000a) Re-validation of Echinostoma miyagawai Ishii, 1932 (Digenea: Echinostomatidae) on the basis of experimental completion of its life-cycle. Systematic Parasitology, 45, 81–108.

    Google Scholar 

  • Kostadinova, A., Gibson, D.I., Biserkov, V. & Ivanova, R. (2000b) A quantitative approach to the evaluation of the morphological variability of two echinostomes, Echinostoma miyagawai Ishii, 1932 and E. revolutum (Frölich, 1802) from Europe. Systematic Parasitology, 45, 1–15.

    Google Scholar 

  • Kosupko, G.A. (1971a) [New data in the bioecology and morphology of Echinostoma revolutum and E. miyagawai (Trematoda: Echinostomatidae).] Byulleten' Vsesoyuznogo Instituta Gel'mintologii im. K.I. Skrjabina, 5, 43–49 (In Russian).

    Google Scholar 

  • Kosupko, G.A. (1971b) [Criteria of the species Echinostoma revolutum, demonstrated on experimental material.] In: Sbornik rabot po gel'mintologii posvyashchen 90-letiyu so dnya rozhdeniya akademika K.I. Skrjabina. Moscow: 'Kolos', pp. 167–175 (In Russian).

    Google Scholar 

  • Kosupko, G.A. (1972) [Morphology and biology of Echinostoma revolutum Frölich, 1802 and Echinostoma miyagawai Ishii, 1932 (Trematoda: Echinostomatidae) studied on experimental material.] Thesis, Moscow, 258 pp [In Russian).

  • Li, W.H. (1993) Unbiased estimation of the rates of synonymous and nonsynonymous substitution. Journal of Molecular Evolution, 36, 96–99.

    Google Scholar 

  • Maddison, D.R. & Maddison, W.P. (2001) MacClade: Analysis of phylogeny and character evolution. Version 4.03. Sinauer Associates, Sunderland, Massachusetts.

    Google Scholar 

  • Morgan, J.A.T. & Blair, D. (1995) Nuclear rDNA ITS sequence variation in the trematode genus Echinostoma: an aid to establishing relationships within the 37-collar-spine group. Parasitology, 111, 609–615.

    Google Scholar 

  • Morgan, J.A.T. & Blair, D. (1998a) Relative merits of nuclear ribosomal internal transcribed spacers and mitochondrial CO1 and ND1 genes for distinguishing among Echinostoma species (Trematoda). Parasitology, 116, 289–297.

    Google Scholar 

  • Morgan, J.A.T. & Blair, D. (1998b) Mitochondrial ND1 gene sequences used to identify echinostome isolates from Australia and New Zealand. International Journal for Parasitology, 28, 493–502.

    Google Scholar 

  • Morgan, J.A.T. & Blair, D. (2000) Molecular biology of echinostomes. In: Fried, B. & Graczyk, T.K. (Eds) Echinostomes as experimental models for biological research. Dordrecht: Kluwer Academic Publishers, pp. 245–266.

    Google Scholar 

  • Pamilo, P. & Bianchi, N.O. (1993) Evolution of the ZFX and ZFY genes: rates and interdependence between the genes. Molecular Biology and Evolution, 10, 271–281.

    Google Scholar 

  • Park, J.T. (1938) A rat trematode, Echinostoma hortense Asada, from Korea. Keijo Journal of Medicine, 9, 283–286.

    Google Scholar 

  • Posada, D. & Crandall, K.A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics, 14, 817–818.

    Google Scholar 

  • Robinson, M., Gouy, M., Gautier, C. & Mouchiroud, D. (1998) Sensitivity of the relative-rate test to taxonomic sampling. Molecular Biology and Evolution, 15, 1091–1098.

    Google Scholar 

  • Saito, S. (1984) Development of Echinostoma hortense in rats, with special reference to the genital organs. Japanese Journal of Parasitology, 33, 191–201.

    Google Scholar 

  • Sorensen, R.E., Curtis, J. & Minchella, D.J. (1998) Intraspecific variation in the rDNA ITS loci of 37-collar-spined echinostomes from North America: implications for sequence-based diagnoses and phylogenetics. Journal of Parasitology, 85, 992–997.

    Google Scholar 

  • Sorensen, R.E., Kanev, I., Fried, B. & Minchella, D.J. (1997) The occurrence and identification ofEchinostoma revolutum from North American Lymnaea elodes snails. Journal of Parasitology, 83, 169–170.

    Google Scholar 

  • Swofford, D.L. (2002) PAUP?: Phylogenetic analysis using parsimony and other methods. Version 4. Sinauer, Sunderland, MA.

    Google Scholar 

  • Telford, M.J., Herniou, E.A., Russell, R.B. & Littlewood, D.T.J. (2000) Changes in mitochondrial genetic codes as phylogenetic characters: two examples from the flatworms. Proceedings of the National Academy of Sciences, 97, 11359–11364.

    Google Scholar 

  • Tkach, V. & Pawlowski, J. (1999) Method for DNA isolation from ethanol-fixed specimens using guanidine thiocyanate. Acta Parasitologica, 44, 147–148.

    Google Scholar 

  • Toledo, R., Munoz-Antoli, C. & Esteban, J.G. (2000) The life-cycle of Echinostoma friedi n. sp. (Trematoda: Echinostomatidae) in Spain and a discussion on the relationships within the 'revolutum' group based on cercarial chaetotaxy. Systematic Parasitology, 45, 199–217.

    Google Scholar 

  • Van Herwerden, L., Blair, D. & Agatsuma, T. (2000) Multiple lineages fo the mitochondrial gene NADH dehydrogenase subunit 1 (ND1) in parasitic helminths: implications for molecular evolutionary studies of facultatively anaerobic eukaryotes. Journal of Molecular Evolution, 51, 339–352.

    Google Scholar 

  • Yamaguti, S., 1941. Zur entwicklungsgeschichte von Echinostoma hortense Asada, 1926, mit besonderer berücksichtigung der struktur der cercarie. Zeitschrift für Parasitenkunde, 12, 271–276.

    Google Scholar 

Download references

Author information

Author notes

  1. A. Kostadinova

    Present address: Marine Zoology Unit, Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, PO Box 22 085, 46071, Valencia, Spain

  2. E.A. Herniou

    Present address: Department of Biology, Imperial College of Science, Technology and Medicine, London, SW7 2AZ, UK

Authors and Affiliations

  1. Department of Biodiversity, Central Laboratory of General Ecology, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113, Sofia, Bulgaria

    A. Kostadinova

  2. Department of Zoology, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK

    E.A. Herniou & D.T.J. Littlewood

  3. Institute of Biological Sciences, University of Wales, SY23 3DA, Wales, UK

    J. Barrett

Authors
  1. A. Kostadinova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E.A. Herniou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Barrett
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D.T.J. Littlewood
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kostadinova, A., Herniou, E., Barrett, J. et al. Phylogenetic relationships of Echinostoma Rudolphi, 1809 (Digenea: Echinostomatidae) and related genera re-assessed via DNA and morphological analyses. Syst Parasitol 54, 159–176 (2003). https://doi.org/10.1023/A:1022681123340

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022681123340

Keywords

Search

Navigation