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Characterization of Staphylococcus species by SDS–PAGE of Whole-Cell and Extracellular Proteins

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Abstract

In this study, a total of fifteen staphylococcal strains belonging to different species were characterized by whole-cell and extracellular protein profiles using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). The results are presented as dendrograms after quantitative analysis of the band patterns with a computer program. Visual inspection of protein bands and cluster analysis of protein patterns of to be used 15 strains, representing 10 Staphylococcus species, showed that whole-cell and extracellular protein profiles differed in several protein bands in Staphylococcus aureus, S. epidermidis, S. simulans, and other species of Staphylococcus; however, the differences were insufficient for reliable differentiation of Staphylococcus species by the SDS–PAGE method.

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Authors and Affiliations

  1. Department of Biology, Faculty of Art and Science, Yüzüncü Yil University, 65080, Van, Turkey

    I. Berber & E. Atalan

  2. Department of Biology, Faculty of Sciences, Ankara University, Tandoğan, 06100, Ankara, Turkey

    C. Cokmus

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  1. I. Berber
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  2. C. Cokmus
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  3. E. Atalan
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Berber, I., Cokmus, C. & Atalan, E. Characterization of Staphylococcus species by SDS–PAGE of Whole-Cell and Extracellular Proteins. Microbiology 72, 42–47 (2003). https://doi.org/10.1023/A:1022221905449

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