Abstract
The individual and combined toxicity of dissolved mercury, copper, lead and cadmium has been investigated by using the Paracentrotus lividus sea-urchin embryo-larval bioassay. Embryogenesis success and early larval growth have been recorded after incubation of fertilised eggs in seawater, both with single metals and binary combinations of Hg with every other metal. For individual metals the ranking of toxicity was Hg > Cu > Pb > Cd, with EC50 values of 21.9, 66.8, 509 and 9240 μg/l, respectively. Lowest observed effect concentrations (LOEC) for early larval growth were approximately three times lower than the EC50 values for Hg, Cu and Pb, and more than two orders of magnitude lower for Cd, emphasizing the danger of underestimating toxicity when only lethal effects are recorded. Marking & Dawson's additive indices ranged from 0.10 to 0.19, indicating additive effects with a slight trend to synergism, which was statistically significant for the Hg–Pb combination only. Hayes' additive indices were within the margins considered acceptable to describe additive interactions.
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Bersier, P.M., Howell, J. and Brunlett, C. (1994). Advanced electroanalytical techniques versus atomic absortion spectrometry inductively coupled plasma atomic-emission spectrometry and inductively coupled plasma mass spectrometry in environmental analyses. Analyst, February 119–219.
Bougis, P., Corre, M.C. and Étienne, M. (1979). Sea-urchin larvae as a tool for assessment of the quality of sea water. Ann. Inst. Océanograph. Paris 55(1), 21–6.
Burstein, H. (1971). Attribute Sampling: Tables and Explanations. New York: Mcgraw-Hill Company.
Carr, R.S. (1996). Sediment quality assessment studies of Tampa Bay, Florida. Environ. Toxicol. Chem. 15(7), 1218–31.
Cassee, F.R., Sühnel, J., Groten, J.P. and Feron, V. (1999).Toxicology of chemical mixtures. In B. Ballantyne, T. Marrs and T. Syversen (eds) General and Applied Toxicology, Vol. 1, pp. 303–19. London: MacMillan.
Chapman, P.M., Dexter, R.N. and Long, E.R. (1987). Synoptic measures of sediments contamination, toxicity and infaunal community composition (the Sediment Quality Triad) in San Francisco Bay. Marine Ecol. Progr. Ser. 37, 75–93.
Coglianese, M.P. and Martin, M. (1981). Individual and interactive effects of environmental stress on the embryonic development of the Pacific Oyster, Crassostrea gigas. I. The toxicity of copper and silver. Marine Environ. Res. 5, 13–27.
Dinnel, P.A. and Stober, Q.J. (1987). Application of the sea urchin sperm bioassay to sewage treatment efficiency and toxicity in marine waters. Marine Environ. Res. 21, 121–33.
Dinnel, P.A., Link, J.M., Stober, Q.J., Letorneau, M.W. and Roberts, W.E. (1989). Comparative sensitivity of sea urchin sperm bioassays to metals and pesticide toxicity tests. Arch. Environ. Contam. Toxicol. 18, 748–55.
Fernández, J.M., Hayes, P.J. and Smyth, M.R. (1992). Analytical voltammetry in environmental science. I. Inorganic species. In M.R. Smyth and J.G. Vos (eds) Analytical Voltametry (Wilson & Wilson's Comprehensive Analytical Chemistry) Vol 27. Amsterdam: Elsevier.
Fernández, N. (1999). Toxicidad del mercurio, cobre, cadmio y plomo sobre la embriog´enesis del erizo de mar (Paracentrotus lividus). MSc Thesis, Universidade de Vigo.
Hayes, W.J. (1991). Dosage and other factors influencing toxicity. In Handbook of Pesticide Toxicology, Vol. 1. General Principles. London: Academic Press.
Heyvang, I. (1994). Toxicité des micropollutants en milieu marin. Mise au point d'un test simplifié basé sur l'utilisation d'oeufs d'embryons et de pluteus de Paracentrotus lividus. Exemples d'applications. IFREMER.
His, E., Budzinski, H., Geffard, O. and Beiras, R. (1997). Effects of a hydrocarbon-polluted sediment on Crassostrea gigas (Thunberg) metamorphosis. Comptes Rendus de l'Académie des Sciences Paris, Série Sciences de la Vie 320, 797–803
His, E., Beiras R. and Seaman, M.N.L. (1999a). The assessment of marine pollution. Bioassays with bivalve embryos and larvae. In A.I. Southward, P.A. Tyler and C.M. Young (eds) Advances in Marine Biology, Vol. 37. London: Academic Press.
His, E., Heyvang, I., Geffard, O. and De Montaudouin, X. (1999b). A comparison between oyster (Crassostrea gigas) and sea urchin (Paracentrotus lividus) larval bioassay for toxicological studies. Water Res. 33(7), 1706–18.
Kobayashi, N. (1981). Comparative toxicity of various chemicals, oil extracts and oil dispersant extracts to Canadian and Japanese sea urchin eggs. Publications of Seto Marine Biology Laboratory XXVI(1/3), 123–33.
MacInnes, J.R. (1980). Response of embryos of the American Oyster, Crassostrea virginica, to heavy metals mixtures. Marine Environ. Res. 4, 217–27.
Marking, L.L. and Dawson, V.K. (1975). Method of assessment of toxicity or efficacy of mixtures of chemicals. US Fish Wildlife Service Invest. Control 67, 1–8
Moreau, C.J., Klerks, P.L. and Haas, C.N. (1999). Interaction between phenanthrene and zinc in the toxicity to the sheepshead minnow (Cyprinodon variegatus). Archiv. Environ. Contam. Toxicol. 37, 251–7.
Morgan, J.D., Mitchell, D.G. and Chapman, P.M. (1986). Individual and combined toxicity of manganese and molybdenum to mussel, Mytilus edulis, larvae. Bull. Environ. Contam. Toxicol. 37, 303–7.
Newman, M.C. (1995). Quantitative Methods in Aquatic Toxicology. Boca Raton: CRC Press.
Pavicic, J. (1980). Interaction of Cd and Zn in relation to oxygen consumption in early stages of marine bivalve molluscs. V Journées d' ÉEtudes des Pollutions 627–34, Cagliari C.I.E.S.M.
Pagano, M., Cipollaro, M., Corsale, G., Esposito, A., Ragucci, E., Giordano, G.G. and Trieff, N.M. (1986). The sea urchin: bioassay for the assessment of damage from environmental contaminants. Community Toxicity Testing, ASTM STP 920, John Cairns, Jr. ed. American Society for Testing and Materials, Philadelphia.
Pereira, S.A., Nascimento, A., Smith, H., Leite, N.L., de Araújo, M.S. and Silva, A. (1998). The combined effects of temperature and metals copper, zinc and mercury on the embryological development of the mangrove oyster, Crassostrea rhizophorae. Ecotoxicol. Environ. Restoration 16(1), 21–32.
Sokal, R. and Rohlf, F. (1981). Biometry, 2nd edn. New York: W.H. Freeman and Company.
Vanegas, C., Espina, S., Botello, A.V. and Villanueva, S. (1997). Acute toxicity and synergism of cadmium and zinc in white shrimp, Penaeus setiferus, juveniles. Bull. Environ. Contam. Toxicol. 58, 87–92.
Warnau, M., Iaccarino, M., De Biase, A., Temara, A., Jangoux, M., Dubois, P. and Pagano, G. (1996). Spermiotoxicity and embryotoxicity of heavy metals in the Echinoid Paracentrotus lividus. Environ. Toxicol. Chem. 15(11), 1931–6.
Warnau, M. and Pagano, G. (1994). Developmental toxicity of PbCl2 in the Echinoid Paracentrotus lividus (Echinodermata) Bull. Environ. Contam. Toxicol. 53, 434–41.
Waterman, A.J. (1937). Effects of salts of heavy metals on development of sea urchin, Arbacia punctulata. Marine Biol. Lab. LXXIII(3), 401–20.
Weltz, B. and Schubert-Jacobs, M. (1991). Evaluation of the flow injection system and optimization of parameters for hydride generation atomic absorption spectrometry. Atom. Spectrosc. 12(4), 91–103.
Zar, J.H. (1984) Biostatistical Analysis, 2nd edn. London: Prentice-Hall International.
Zhadan, P.M., Vashchenco, M.A., Malakhov, V.V.,Medvedeva, L.A. and Gareyeva, R.V. (1992). The effect of environmental pollution, hydrocarbons and heavy metals on reproduction of sea urchins and bivalves. In V.I. Ilyichev and V.V. Anikiev (eds) Oceanic and Anthropogenic Controls of Life in the Pacific Ocean, pp. 267–86. Dordrecht: Kluwer Academic Publishers.
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Fernández, N., Beiras, R. Combined Toxicity of Dissolved Mercury With Copper, Lead and Cadmium on Embryogenesis and Early Larval Growth of the Paracentrotus Lividus Sea-Urchin. Ecotoxicology 10, 263–271 (2001). https://doi.org/10.1023/A:1016703116830
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DOI: https://doi.org/10.1023/A:1016703116830