Abstract
The effects of light intensity, inoculum volume, sodium nitrate and carbon dioxide concentrations on the growth of Haematococcus pluvialis were investigated using response surface methodology (RSM). All the four variables exhibited significant effects on growth and can be related (r ≥ 0.926, P ≤ 0.01) by a second-order polynomial consisting of linear, quadratic and interaction terms. The total quadratic effect (P ≤ 0.01) dominates over the total linear effect (P ≤ 0.01) but the role of interaction terms (P ≤ 0.10) is marginal. The optimum values of these variables were: carbon dioxide 1.54%, sodium nitrate 1.06 g/l, inoculum volume 24.97% and light intensity 2.42 klux; the predicted maximum value for the yield of biomass was 0.51 g/l (dry weight).
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Sarada, R., Bhattacharya, S. & Ravishankar, G. Optimization of culture conditions for growth of the green alga Haematococcus pluvialis . World Journal of Microbiology and Biotechnology 18, 517–521 (2002). https://doi.org/10.1023/A:1016349828310
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DOI: https://doi.org/10.1023/A:1016349828310