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Mapping QTLs conferring salt tolerance during germination in tomato by selective genotyping

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Abstract

This study was conducted to identify genomic regions (quantitative trait loci, QTLs) affecting salt tolerance during germination in tomato. Germination response of an F2 population of a cross between UCT5 (Lycopersicon esculentum, salt-sensitive) and LA716 (L. pennellii, salt-tolerant) was evaluated at a salt-stress level of 175 mM NaCl + 17.5 mM CaCl2 (water potential ca. −950 kPa). Germination was scored visually as radicle protrusion at 6 h intervals for 30 consecutive days. Individuals at both extremes of the response distribution (i.e., salt-tolerant and salt-sensitive individuals) were selected. The selected individuals were genotyped at 84 genetic markers including 16 isozymes and 68 restriction fragment length polymorphisms (RFLPs). Trait-based marker analysis (TBA) which measures changes (differences) in marker allele frequencies in selected lines was used to identify marker-linked QTLs. Eight genomic regions were identified on seven tomato chromosomes bearing genes (QTLs) with significant effects on this trait. The results confirmed our previous suggestion that salt tolerance during germination in tomato is polygenically controlled. The salt-tolerant parent contributed favorable QTL alleles on chromosomes 1, 3, 9 and 12 whereas the salt sensitive parent contributed favorable QTL alleles on chromosomes 2, 7 and 8. The identification of favorable alleles in both parents suggests the likelihood of recovering transgressive segregants in progeny derived from these parental genotypes. The results can be used for marker-assisted selection and breeding of salt-tolerant tomatoes.

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Author notes

  1. T. Stoltz

    Present address: Applied Phytologics, Inc., 4110 N. Freeway Blvd., Sacramento, CA, 95834, USA

Authors and Affiliations

  1. Department of Horticulture, The Pennsylvania State University, 103 Tyson Building, University Park, PA, 16802, USA

    M. R. Foolad, T. Stoltz & C. Dervinis

  2. Section of Molecular and Cell Biology, University of California, Davis, CA, 95616, USA

    R. L. Rodriguez

  3. Department of Vegetable Crops, University of California, Davis, CA, 95616, USA

    R. A. Jones

Authors
  1. M. R. Foolad
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  2. T. Stoltz
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  3. C. Dervinis
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  4. R. L. Rodriguez
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  5. R. A. Jones
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Correspondence to M. R. Foolad.

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Foolad, M.R., Stoltz, T., Dervinis, C. et al. Mapping QTLs conferring salt tolerance during germination in tomato by selective genotyping. Molecular Breeding 3, 269–277 (1997). https://doi.org/10.1023/A:1009668325331

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