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A genetic analysis of quantitative resistance to late blight in potato: towards marker-assisted selection

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Abstract

Late blight caused by the oomycete Phytophthora infestans is the most important fungal disease in potato cultivation worldwide. Resistance to late blight is controlled by a few major genes (R genes) which can be easily overcome by new races of P. infestans and/or by an unknown number of genes expressing a quantitative type of resistance which may be more durable. Quantitative resistance of foliage to late blight was evaluated in five F1 hybrid families originating from crosses among seven different diploid potato clones. Tuber resistance was evaluated in four of the families. Two of the families were scored for both foliage maturity and vigour. The five families were genotyped with DNA-based markers and tested for linkage with the traits analysed. QTL (quantitative trait locus) analysis identified at least twelve segments on ten chromosomes of potato having genes that affect reproducibly foliage resistance. Two of those segments also have major R genes for resistance to late blight. The segments are tagged by 21 markers that can be analyzed based on PCR (polymerase chain reaction) with specific oligonucleotide primers. One QTL was detected for tuber resistance and one for foliage vigour. Two QTLs were mapped for foliage maturity. Major QTL effects on foliage and tuber resistance to late blight and on foliage maturity and vigour were all linked with marker GP179 on linkage group V of potato. Plants having alleles at this QTL, which increased foliage resistance, exhibited decreased tuber resistance, later maturity and more vigour.

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References

  1. Becker-Andre M, Schulze-Lefert P, Hahlbrock K: Structural comparison, modes of expression, and putative cis-acting elements of the two 4-coumarate:CoA ligase genes in potato. J Biol Chem 266: 8551–855 (1991).

    PubMed  Google Scholar 

  2. Bendahmane A, Kanyuka K, Baulcombe DC: High resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato. Theor Appl Genet 95: 153–162 (1997).

    Article  Google Scholar 

  3. Bianchi A, Morandi A: A case of balanced lethal factors in maize. Heredity 17: 409–414 (1962).

    Google Scholar 

  4. Cohen Y, Reuveni M: Occurrence of metalaxyl-resistant isolates of Phytophthora infestans and of genes controlling immunity in Solanum demissum derivatives. Euphytica 2: 173–240 (1983).

    Google Scholar 

  5. Collins A, Milbourne D, Ramsay L, Meyer R, Chatot-Balandras C, Oberhagemann P, de Jong W, Gebhardt C, Bonnel E, Waugh R: QTL for field resistance to late blight in potato are strongly correlated with earliness and vigour. Mol Breed 0: 000–000 (1999).

    Google Scholar 

  6. de Jong W, Forsyth A, Leister D, Gebhardt C, Baulcombe DC: A potato hypersensitive resistance gene against potato virus X maps to a resistance gene cluster on chromosome 5. Theor Appl Genet 95: 246–252 (1997).

    Article  Google Scholar 

  7. Dowley LJ, Carnegie SF, Balandras-Chatot C, Ellisseche D, Gans P, Schober-Butin B, Wustman R: Guide-line for the evaluation of potato cultivars for resistance to foliage blight (field test). Proceedings EAPR Pathology Meeting, Umea (Sweden) (1998).

  8. El-Kharbotly A, Leonards-Schippers C, Huigen DJ, Jacobsen E, Pereira A, Stiekema WJ, Salamini F, Gebhardt C: Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents. Mol Gen Genet 242: 749–754 (1994).

    PubMed  Google Scholar 

  9. El-Kharbotly A, Palomino-Sánchez C, Salamini F, Jacobsen E, Gebhardt C: R6 and R7 alleles of potato conferring race-specific resistance to Phytophthora infestans (Mont.) de Bary identified genetic loci clustering with the R3 locus on chromosome XI. Theor Appl Genet 92: 880–884 (1996).

    Article  Google Scholar 

  10. Feinberg AP, Vogelstein B: A technique for radio labeling DNA restriction endonuclease fragments to high specific activity (Addendum). Anal Biochem 137: 266–267 (1984).

    PubMed  Google Scholar 

  11. Feltkamp D, Masterson R, Starke J, Rosahl S: Analysis of the involvement of ocs-like bZip-binding elements in the differential strength of the bidirectional mas 1010 promoter. Plant Physiol 105: 259–268 (1994).

    PubMed  Google Scholar 

  12. Fritzemeier K-H, Cretin C, Kombrink E, Rohwer F, Taylor J, Scheel D, Hahlbrock K: Transient induction of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase mRNAs in potato leaves infected with virulent or avirulent races of Phytophthora infestans. Plant Physiol 85: 34–41 (1987).

    Google Scholar 

  13. Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F: RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78: 65–75 (1989).

    Article  Google Scholar 

  14. Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F: RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83: 49–57 (1991).

    Article  Google Scholar 

  15. Gebhardt C, Ritter E, Salamini F: RFLP map of the potato. In: Phillips RL, Vasil IK (eds) DNA-Based Markers in Plants, pp. 271–285. Kluwer Academic Publishers, Dordrecht/ Boston/ London (1994).

    Google Scholar 

  16. Gebhardt C, Eberle B, Leonards-Schipppers C, Walkemeier B, Salamini F: Isolation, characterization and RFLP linkage mapping of a DNA repeat family of Solanum spegazzinii by which chromosome ends can be localized on the genetic map of potato. Genet Res Camb 65: 1–10 (1995).

    Google Scholar 

  17. Johal GS, Hulbert SH, Briggs SP: Disease lesion mimics of maize: amodel for cell death in plants. BioEssays 17: 685–692 (1995).

    Google Scholar 

  18. Leister D, Ballvora A, Salamini F, Gebhardt C: A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nature Genet 14: 421–429 (1996).

    Article  PubMed  Google Scholar 

  19. Leonards-Schippers C, Gieffers W, Salamini F, Gebhardt C: The R1 gene conferring race-specific resistance to Phytophthora infestans in potato is located on potato chromosome V. Mol Gen Genet 233: 278–283 (1992).

    Article  PubMed  Google Scholar 

  20. Leonards-Schippers C, Gieffers W, Schäfer-Pregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C: Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in an allogamous plant species. Genetics 137: 67–77 (1994).

    PubMed  Google Scholar 

  21. Li X, van Eck HJ, Rouppe van der Voort JNAM, Huigen DJ, Stam P, Jacobsen E: Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4. Theor Appl Genet 96: 1121–1128 (1998).

    Article  Google Scholar 

  22. Logemann J, Mayer JE, Schell J, Willmitzer L: Differential expression of genes in potato tubers after wounding. Proc Natl Acad Sci USA 85: 1136–1140 (1988).

    Google Scholar 

  23. Mann HB, Whitney DR: On a test of wether one of two random variables is stochastically larger than the other. Ann Math Statist 18: 50–60 (1947).

    Google Scholar 

  24. Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD: Map based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262: 1432–1436 (1998).

    Google Scholar 

  25. Meksem K, Leister D, Salamini F, Gebhardt C: A high resolution map of chromosome V of potato based on RFLP and AFLP markers in the vicinity of the R1 locus. Mol Gen Genet 249: 74–81 (1995).

    Article  PubMed  Google Scholar 

  26. Milbourne D, Meyer R, Collins A, Ramsay L, Gebhardt C and Waugh R: Isolation, characterization and mapping of simple sequence repeat loci in potato. Mol Gen Genet 259: 233–245 (1998).

    Google Scholar 

  27. Niewöhner J, Salamini F, Gebhardt C: Development of PCR assays diagnostic for RFLP marker alleles closely linked to alleles Gro1 and H1, conferring resistance to the root cyst nematode Globodera rostochiensis in potato. Mol Breed 1: 65–78 (1995).

    Google Scholar 

  28. Robertson DS: A possible technique for isolating genomic DNA for quantitative traits in plants. J Theor Biol 117: 1–10 (1985).

    Google Scholar 

  29. Ross H: Potato breeding: problems and perspectives. J Plant Breed, Suppl. 13 (1986).

  30. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491 (1988).

    PubMed  Google Scholar 

  31. SAS Institute INC., SAS Language: Reference, Version 6, 4th ed., vol. 2, 1042 pp. SAS Institute, Cary, NC (1990).

    Google Scholar 

  32. Schäfer-Pregl R, Ritter E, Concilio L, Hesselbach J, Lovatti L, Walkemeier B, Thelen H, Salamini F, Gebhardt C: Analysis of quantitative trait loci (QTL) and quantitative trait alleles (QTA) for potato tuber yield and starch content. Theor Appl Genet 97: 834–846 (1998).

    Article  Google Scholar 

  33. SPSS® Inc.: Base System Syntax Reference Guide, Release 5.0 (1992).

  34. Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Röder MS, Wing RA, Wu W, Young DN: High density molecular linkage maps of the tomato and potato genomes. Genetics 132: 1141–1160 (1992).

    PubMed  Google Scholar 

  35. Taylor JT, Fritzemeier H, Häuser I, Kombrink E, Rohwer F: Structural analysis and activation by fungal infection of a gene encoding a pathogenesis-related protein in potato. Mol Plant-Microbe Interact 3: 72–77 (1990).

    PubMed  Google Scholar 

  36. Van der Leij FR, Visser RG, Ponstein AS, Jacobsen E, Feenstra WJ: Sequence of the structural gene for granule-bound starch synthase of potato (Solanum tuberosum L.) and evidence for a single point deletion in the amf allele. Mol Gen Genet 228: 240–248 (1991).

    Article  PubMed  Google Scholar 

  37. Wastie RL: Breeding for resistance. In: Ingram DS, Williams PH (eds), Advances in Plant Pathology 7. Phytophthora infestans, the cause of late blight of potato, pp. 193–223. Academic Press, London (1991).

    Google Scholar 

  38. Wemmer T, Kaufmann H, Kirch H-H, Schneider K, Lottspeich F, Thompson RD: The most abundant soluble basic protein of the stylar transmitting tract in potato (Solanum tuberosum L.) is an endochitinase. Planta 194: 264–273 (1994).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne-Weg 10, D-50829, Köln, Germany

    Petra Oberhagemann, Ralf Schäfer-Pregl, Dorothee Wegener, Carmen Palomino, Francesco Salamini & Christiane Gebhardt

  2. GERMICOPA, 1, Allee Loeiz Herrieu, 29334 , Quimper Cedex, France

    Catherine Chatot-Balandras & Eric Bonnel

Authors
  1. Petra Oberhagemann
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  2. Catherine Chatot-Balandras
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  3. Ralf Schäfer-Pregl
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  4. Dorothee Wegener
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  5. Carmen Palomino
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  6. Francesco Salamini
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  7. Eric Bonnel
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  8. Christiane Gebhardt
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Oberhagemann, P., Chatot-Balandras, C., Schäfer-Pregl, R. et al. A genetic analysis of quantitative resistance to late blight in potato: towards marker-assisted selection. Molecular Breeding 5, 399–415 (1999). https://doi.org/10.1023/A:1009623212180

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