Abstract
This study focuses on gene expression of porcine circovirus (PCV) in order to identify viral genes and their corresponding mRNA transcripts. By northern blot analysis, the existence of three mRNAs could be demonstrated. Two mRNAs are encoded by the viral (−)-strand and one is encoded by the viral (+)-strand. The (+)-strand encoded mRNA transcript is 990 nucleotides (nt) long and corresponds to the open reading frame (ORF) 1, as shown by S1 mapping. The start point of this transcript is located at pos. 1238, as determined by primer extension analysis and rapid amplification of cDNA ends (RACE). The transcript is spliced as shown by direct reverse sequencing and RACE. It contains an untranslated “leader”-sequence 119 nt in size (pos. 1238 to 1120) which is joined to exon 2 of the ORF 1 transcript at pos. 737. The transcriptional regulatory elements have been identified functionally by CAT assays. They are located within a 258 base points (bp) fragment (pos. 1168 to 1425).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Allan G.M., Pig Journal 37, 14-19, 1996.
Aviv H. and Leder P., Proceedings of the National Academy of Sciences 69, 1408-1412, 1972.
Berk A.J. and Sharp P.A., Cell 14, 695-711, 1978.
Blaess G., Lorenz R., Mankertz J., Tischer I., Buhk H.-J., and Mankertz A., submitted.
Boevink P., Chu P.W.G., and Keese P., Virology 207, 354-361, 1995.
Breathnach R. and Chambon P., Annual Review of Biochemistry 50, 349-383, 1981.
Gorman C.M., Moffat L.F., and Howard B.H., Molecular and Cellular Biology 2, 1044-1051, 1982.
Harding R.M., Burns T.M., Hafner G., Dietzgen R.G., and Dale J.L., Journal of General Virology 74, 323-328, 1993.
Hayashi M., Aoyama A., Richardson D.L., and Hayashi M.N., Biology of the bacteriophage ΦX174. in: Calendar, R., The bacteriophages, Volume 2, Plenum Press, New York and London, 1-71, 1988.
Kadonaga J.T., Jones K.A., and Tjian R., Trends in Biochemical Sciences 11, 20-23, 1986.
Kozak M., Microbiological Reviews 47, 1-45, 1983.
Lazarowitz S., Crit Rev Plant Sci 11, 327-349, 1992.
Mankertz A., Persson F., Mankertz J., Blaess G., and Buhk H.-J., Journal of Virology 71(3), 2562-2566, 1997
Mankertz A., Mankertz J., Wolf K., and Buhk H.-J., Journal of General Virology, 79, 381-384, 1998.
Meehan B.M., Creelan J.L., McNulty M.S., and Todd D., Journal of General Virology 78, 221-227, 1997.
Monroy A.F., Focus 10(1), 14, 1988.
Ritchie B.W., Niagro F.D., Lukert P.D., Steffens III, W.L., and Latimer K.S., Virology 171, 83-88, 1989.
Rohde W., Randles J.W., Langridge P., and Hanold D., Virology 176, 648-651, 1990.
Sharp P.A., Konarska M.M., Grabowski P.J., Lamond A.I., Marciniak R., and Seiler S.R., Splicing of messenger RNA precursors. Cold Spring Harbour Symposia on Quantitative Biology LII, 277-285, 1987.
Tischer I., Rasch R., and Tochtermann G., Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene, Series A 226, 153-167, 1974.
Tischer I., Gelderblom H., Vettermann W., and Koch M.A., Nature 295, 64-66, 1982.
Tischer I., Mields W., Wolff D., Vagt M., and Griem W., Archives of Virology 91, 271-276, 1986.
Tischer I., Peters D., Rasch R., and Pociuli S., Archives of Virology 96, 39-57, 1987.
Tischer I. and Buhk H.-J., Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene, Series A 270, 280-287, 1988.
Tischer I., Bode L., Peters D., Pociuli S., and Germann B., Archives of Virology 140(4), 737-743, 1995
Todd D., Creelan J.L., Mackie D.P., Rixon F., and McNulty M.S., Journal of General Virology 71, 819-823, 1990.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mankertz, J., Buhk, HJ., Blaess, G. et al. Transcription Analysis of Porcine Circovirus (PCV). Virus Genes 16, 267–276 (1998). https://doi.org/10.1023/A:1008022521329
Issue Date:
DOI: https://doi.org/10.1023/A:1008022521329