Abstract
We investigated some mechanisms, which allow maize genotypes to adapt to soils which are low in available P. Dry matter production, root/shoot-ratio, root length and root exudation of organic acids and acid phosphatase were investigated in four maize genotypes grown under P-deficient and P-sufficient conditions in sterile hydroponic culture. A low-P tolerant, an acid-tolerant and a low-P susceptible genotype of maize were compared with a Swiss commercial cultivar. The study found increased root development and increased exudation of acid phosphatase under P-deficient conditions in all maize genotypes, except for the Swiss cultivar. Effects on root formation and acid phosphatase were greater for the low-P tolerant than for the low-P susceptible, and the acid soil tolerant genotypes. Organic acid contents in root tissues were increased under P deficiency and related to increased PEPC activity. However, the increase in contents was associated with an increase in exudation for the low-P tolerant genotype only. The low-P susceptible genotype was characterized by high organic acid content in roots and low organic acid exudation. The organic acids content in the phloem exudates of shoots was related to root exudation under different P supply, to the difference between lines in organic acids root content, but not to the low-P tolerance or susceptibility of maize genotypes.
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References
Anghinoni I and Barber S A 1980 Phosphorus influx and growth characteristics of corn roots as influenced by phosphorus supply. Agron. J. 72, 685–688.
Bertrand I 1998 Importance of the protons release on the mobilisation of mineral phosphorus and iron by roots. Study of models minerals: calcite and goethite. Ph.D Thesis, Université Aix-Marseille III, France.
Braum S M and Helmke P A 1995 White lupin utilizes soil phosphorus that is unavailable to soybean. Plant Soil 176, 95–100.
Friesen D K, Rao I M, Thomas R J, Oberson A and Sanz J I 1997 Phosphorus acquisition and cycling in crop and pasture systems in low fertility tropical soils. Plant Soil 196, 289–294.
Gardner W K, Barber D A and Parbery D G 1983 The acquisition of phosphorus by Lupinus albus L. III. The probable mechanism by which phosphorus movement in the soil/root interface is enhanced. Plant Soil 70, 107–124.
Gerke J 1992 Phosphate, aluminum and iron in the soil solution of three different soils in relation to varying concentrations of citric acid. Z. Pflanzenernaehr. Bodenk. 155, 339–343.
Helal H M and Sauerbeck D 1987 Phosphatase-Aktivität von Pflanzenwurzeln und Böden in Abhängigkeit von der P-Versorgung. VDLUFA-Schriftenreihe 23, 195–201.
Hoffland E, Findenegg G R and Nelemans J A 1989 Solubilization of rock phosphate by rape. Plant Soil 113, 155–160.
Hoffland E, Nelemans J A and Findenegg G R 1990 Origin of organic acids exuded by roots of phosphorus stressed rape (Brassica napus) plants. In Plant Nutrition-Physiology and Applications. Ed. ML Van Beusichem, pp 179–183. Kluwer Academic Publishers, Norwell.
Hoagland, D R and Arnon I R 1938 The water culture method for growing plants without soils. Circ. Calif. Agric. Exp. Stn. No. 347.
Hrazdina G and Zobel A M 1991 Cytochemical localization of enzymes in plant cells. Bot. Rev. 129, 269–322. 264
Hue N V, Craddock G R and Adams F 1986 Effect of organic acids on Al toxicity in subsoils. Soil Sci. Soc. Am. J. 50, 28–34.
Imas P, Bar-Yosef B, Kafkafi U and Ganmore-Neumann R 1997a Release of carboxylic anions and protons by tomato roots in response to ammonium nitrate ratio and pH in nutrient solution. Plant Soil 191, 27–34.
Imas P, Bar-Yosef B, Kafkafi U and Ganmore-Neumann R 1997b Phosphate induced carboxylate and proton release by tomato roots. Plant Soil 191, 35–39.
John M K 1970 Colorimetric determination of phosphorus in soil and plant materials with ascorbic acid. Soil Sci. 4, 214–220.
Johnson J F, Vance C P and Allan D L 1996 Phosphorus deficiency in Lupinus albus. Altered lateral root development and enhanced expression of phosphoenolpyruvate carboxylase. Plant Physiol. 112, 31–41.
Jones D L and Darrah P R 1994a Amino-acid influx at the soil-root interface of Zea mays L. and its implications in the rhizosphere. Plant Soil 163, 1–12.
Jones D L and Darrah P R 1994b Role of root derived organic-acids in the mobilization of nutrients from the rhizosphere. Plant Soil 166, 247–257.
Jones D L and Darrah P R 1995 Influx and efflux of organic acids across the soil—root interface of Zea mays L. and its implications in rhizosphere C flow. Plant Soil 173, 103–109.
Khamis S, Chaillou S and Lamaze T 1990 CO2 assimilation and partitioning of carbon in maize deprived of orthophosphate. J. Exp. Bot. 41, 1619–1625.
King R W and Zeevaart J A D 1974 Enhancement of phloem exudation from cut petioles by chelating agents. Plant Physiol. 53, 96–103.
Kirkby E A and Knight A H 1977 Influence of the level of nitrate nutrition on ion uptake and assimilation, organic acid accumulation, and cation-anion balance in whole tomato plants. Plant Physiol. 60, 349–353.
Kraffczyk I, Trolldenier G and Beringer H 1984 Soluble roots exudates of maize: Influence of potassium supply and rhizosphere micro-organisms. Soil Biol. Biochem. 16, 315–322.
Kummerova M 1986 Localization of acid phosphatase in maize root under phosphorus deficiency. Biol. Plant. 28, 270–274.
Lynch J P 1995 Root architecture and plant productivity. Plant physiol. 109, 7–13.
Lynch J M and Whipps J M 1989 Substrate flow in the rhizosphere. In The Rhizosphere and Plant Growth. Eds. DL Keister and PB Cregan, pp 15–24. Kluwer Academic Publishers, Dortrecht, The Netherlands.
Marschner H 1995 Mineral nutrition of higher plants. Academic Press, London 2nd edn. 889 p.
Matsumoto H, Okada K and Takahashi E 1979 Excretion products of maize roots from seedling to seed development stage. Plant Soil 53, 17–26.
McCully M E 1999 Roots in soil: Unearthing the complexities of roots and their rhizospheres. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50, 695–718.
Mench M, Morel J L, Guckert A and Guillet B 1988 Metal binding with root exudates of low molecular weight. J. Soil Sci. 39, 521–527.
Mollier A and Pellerin S 1999 Maize root system growth and development as influenced by phosphorus deficiency. J. Exp. Bot. 55(333), 487–497.
Nagarajah S, Posner A M and Quirk J P 1970 Competitive adsorptions of phosphate with polygalacturonate and other organic anions on kaolinite and oxide surfaces. Nature (London) 228, 83–84.
Pearson C J, Volk R J and Jackson W A 1981 Daily changes in nitrate influx, efflux and metabolism in maize and pearl millet. Planta 152, 319–324.
Petersen W and Böttger M 1991 Contribution of organic acids to the acidification of the rhizosphere of maize seedlings. Plant Soil 132, 159–163.
Raghothama K G 1999 Phosphate acquisition. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50, 665–693.
Rosolem C A, Assis J S and Santiago A D 1994 Root growth and mineral nutrition of corn hybrids as effected by phosphorus and lime. Com. Soil Sci. Plant Anal. 25, 2491–2499.
Schopfer P 1984 Photomorphogenesis. In Advanced Plant Physiology. Ed. MB Wilkins, pp 380–407. Pitman, London, Marshfield, Melbourne.
Staunton S and Leprince F 1996 Effect of pH and some organic anions on the solubility of soil phosphate: implications for P bioavailability. Eur. J. Soil. Sci. 47, 231–239.
SYSTAT 1994 SPSS Inc., Chicago.
Tabatabai M A 1982 Soil enzymes. In Methods of Soil Analysis. Part 2, Chemical and Microbiological Properties. Eds. AL Page, RH Miller and DR Keeney, pp 923–931. Agronomy ASA, Madison, Wisconsin, USA.
Tadano T and Sakai H 1991 Secretion of acid phosphatase by the roots of several crop species under phosphorus-deficient conditions. Soil Sci. Plant Nutr. 37(1), 129–140.
Tarafdar J C and Jungk A 1987 Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol. Fertil. Soils 3, 199–204.
Uren N C and Reisenauer H M 1988 The role of root exudates in nutrient acquisition. Adv. Plant Nutr. 3, 79–114.
Vance C P, Stade S and Maxwell C A 1983 Alfalfa root nodule carbon dioxide fixation. I. Association with nitrogen fixation and incorporation into amino acids. Plant Physiol. 72, 469–473.
Zhang F S, Ma J and Cao Y P 1997 Phosphorus deficiency enhances root exudation of low-molecular weight organic acids and utilization of sparingly soluble inorganic phosphates by radish (Raghanus satiuvs L.) and rape (Brassica napus L.) plants. Plant Soil 196, 261-264.
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Gaume, A., Mächler, F., De León, C. et al. Low-P tolerance by maize (Zea mays L.) genotypes: Significance of root growth, and organic acids and acid phosphatase root exudation. Plant and Soil 228, 253–264 (2001). https://doi.org/10.1023/A:1004824019289
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DOI: https://doi.org/10.1023/A:1004824019289