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Metal speciation in xylem exudate of Lycopersicon esculentum Mill — technetium

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Abstract

This paper deals with a new analytical approach for studying metal speciation in the xylem, as applied to the nuclear waste metal technetium (99Tc) in tomato plants (Lycopersicon esculentum Mill, cv. Tiny Tim). Special attention was paid to sampling protocols and sample handling so that metal oxidation/reduction processes during analytical procedures did not influence the results. Marker Tc-ligand complexes were synthesized in vitro (Tc-cysteine, Tc-histidine, Tc-citrate, and Tc-nicotianamine) and sampling was performed under both argon and atmospheric conditions. Analyses were carried out using paper chromatography, gel electrophoresis (PAGE), and reversed phase ion-pair chromatography (RP-IPC). The results show Tc to be present predominantly in the TcO4 form in the xylem, indicating transport similarities with molybdenum and chromium. Probably, the approach used is also valuable in other metal-speciation studies in the xylem.

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Krijger, G.C., Van Vliet, P.M. & Wolterbeek, H.T. Metal speciation in xylem exudate of Lycopersicon esculentum Mill — technetium. Plant and Soil 212, 163–171 (1999). https://doi.org/10.1023/A:1004607813710

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