biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:549-558, 2000 | DOI: 10.1023/A:1002813530741

The Substrate Preference and Histochemical Localization Argue against the Direct Role of Cucumber Stress-Related Anionic Peroxidase in Lignification

V. Repka1,*, D. Štetková1, I. Fischerová1
1 Laboratory of Molecular Biology and Virology, Research Institute of Viticulture and Enology, (C.R.I.V.E.), Bratislava, Slovakia e-mail

The substrate preference and the localization of cucumber (Cucumis sativus L.) stress-related anionic peroxidase (srPRX) were investigated in order to assess whether this activity correlates with the lignification. The results showed that none of the purified srPRX isoenzymes (PRX 1 -3) could oxidize the lignin monomer analog syringaldazine. The srPRX immunospecific signal was found to be highly abundant in both the extrafascicular and fascicular phloem regions in cucumber stem and leaf petiole. In Nicotiana, Petunia and Dahlia, the srPRX homologs were specifically deposited in both outer and inner phloem elements of stem and in both abaxial and adaxial phloem of leaf stems. The srPRX mRNA expression analysis showed similar pattern as for immunolocalization. The subcellular localization of immunospecific srPRX demonstrated that at least part of the peroxidase could be ionically-bound to phloem cell wall.

Keywords: Cucumis; Dahlia; electrophoresis; immunoblotting; Nicotiana; tissue print Western; tissue print Northern
Subjects: cucumber, stress-related anionic peroxidase localization, lignification; Cucumis sativus; Dahlia variabilis; lignification, stress-related anionic peroxidase; Nicotiana tabacum; Petunia hybrida; stress-related anionic peroxidase, lignification; tissue print, Northern, Western

Published: December 1, 2000  Show citation

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Repka, V., Štetková, D., & Fischerová, I. (2000). The Substrate Preference and Histochemical Localization Argue against the Direct Role of Cucumber Stress-Related Anionic Peroxidase in Lignification. Biologia plantarum, 43(4), 549-558. doi: 10.1023/A:1002813530741
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