Abstract
Two types of IgG anti-DNA antibodies exhibiting DNA-hydrolyzing activity have been isolated from blood serum of patients with systemic lupus erythematosus. This DNase activity of antibodies differs from serum DNases by the non-processive mode, temperature resistance, pH optimum, and the rate of DNA hydrolysis. It is suggested that the anti-DNA antibody molecule possessing DNase activity contains two sites: one site determines specificity of antibody–DNA interaction, whereas the other is responsible for manifestation of the catalytic activity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Pauling, L. (1948) Am. Scientist, 36, 51-59.
Gololobov, G. V., Shuster, A. M., Kvashuk, O. A., and Gabibov, A. G. (1991) Mol. Biol. (Moscow), 25, 593-602.
Shuster, A. M., Gololobov, G. V., Kvashuk, O. A., Bogomolova, A. E., Smirnov, I. V., and Gabibov, A. G. (1992) Science, 256, 665-667.
Vlassov, A. V., Baranovsky, A. G., Kanyshkova, T. G., Prints, A. V., Zabara, V. G., Naumov, V. A., Breusov, A. A., Giege, R., Buneva, V. N., and Nevinsky, G. A. (1998) Mol. Biol. (Moscow), 32, 559-569.
Bronstein, I. B., Shuster, A. M., Gololobov, G. V., Gromova, I. I., Kvashuk, O. A., Belostotstaya, K. M., Alekberova, Z. S., Prokaeva, T. V., and Gabibov, A. G. (1992) FEBS Lett., 314, 259-263.
Baranovsky, A. G., Matyushin, V. G., Vlassov, A. V., Zabara, V. G., Naumov, V. A., Giege, R., Buneva, V. N., and Nevinsky, G. A. (1997) Biochemistry (Moscow), 62, 1358-1366.
Gololobov, G. V., Mikhalp, S. V., Starov, A. V., Kolesnikov A. V., and Gabibov, A. G. (1994) Appl. Biochem. Biotechnol., 47, 305-315.
Kozyr, A. V., Kolesnikov, A. V., Yakhnina, E. I., Astsaturov I. A., Varlamova, E. Yu., Kirillov, E. V., and Gabibov, A. G. (1996) Byull. Eksp. Biol. Med., No. 2, 204-206.
Paul, S., Volle, D. J., Beach, C. M., Johnson, D. R., Powell, M. J., and Massey, R. J. (1989) Science, 244, 1158-1161.
Kanyshkova, T. G., Semenov, D. V., Vlassov, A. V., Shipitsyn, M. V., Yamkovoi, V. I., Buneva, V. N., and Nevinsky, G. A. (1997) Mol. Biol. (Moscow), 31, 1082-1091.
Nevinsky, G. A., Breusov, A. A., Baranovskii, A. G., Prints A. V., Kanyshkova, T. G., Galvita, A. V., Naumov, V. A., and Buneva, V. N. (2001) Med. Sci. Monit., 7, 201-211.
Kuznetsova, N. N., and Vinter, V. G. (1997) Methods of Gene Engineering [in Russian], Bioinformservis, Moscow.
Sattarova, L. I., Gafiyatullina, L. A., Agliullina, D. G., and Vinter, V. G. (1994) Biotekhnologiya, 11/12, 38-41.
Laemmli, U. K. (1970) Nature, 227, 680-685.
Osterman, L. A. (1983) Study of Biological Macromolecules by Isoelectrofocusing, Immunoelectrophoresis and Radioisotope Methods [in Russian], Nauka, Moscow.
Temnikov, D. A. (1999) Pribor. Tekh. Eksp., 6, 59-62.
Paoletti, C., Le Pek, J. B., and Lehmman, J. R. (1971) J. Mol. Biol., 55, 75-99.
Okamura, M., Kanayama, Y., Amastu, K., Negoro, N., Kohda, S., Takeda, T., and Inoue, T. (1993) Ann. Rheum. Dis., 52, 14-20.
Winkler, T., Henschel, T., Kalies, I., Baenkler, H., Skvaril F., and Kalden, J. (1988) Clin. Exp. Immunol., 72, 434-439.
Shapot, V. S. (1968) Nucleases [in Russian], Meditsina, Moscow.
Shevelev, I. V., Legina, O. K., Tutaev, K. Yu., and Krutyakov, V. M. (1998) Mol. Biol. (Moscow), 32, 741-744.
Buneva, V. N., Andrievskaya, O. A., Romannikova, I. V., Gololobov, G. V., Yadav, R. P., Yamkovoi, V. I., and Nevinsky, G. A. (1994) Mol. Biol. (Moscow), 28, 738-741.
Kit, Yu. Ya., Semenov, D. V., and Nevinsky, G. A. (1995) Mol. Biol. (Moscow), 29, 893-906.
Semenov, D. V., Kanyshkova, T. G., Kit, Yu. A., Khlimankov, D. Yu., Akimzhanov, A. M., Gorbunov, D. A., Buneva, V. N., and Nevinsky, G. A. (1998) Biochemistry (Moscow), 63, 935-943.
Savel'ev, A. N., Eneyskaya, E. V., Shabalin, K. A., Filatov M. V., and Neustroev, K. N. (1999) Protein Peptide Lett., 6, 179-184.
Murray, R., Granner, D., Mayes, P., and Rodwell, V. (1993) Harper's Biochemistry, 21st Edn. [Russian translation], Mir, Moscow.
Roitt, I., Brostoff, J., and Male, D. (2000) Immunology [Russian translation], Mir, Moscow.
Blow, D. (2000) Structure with Folding and Design, 8, R77-R81.
Baranovsky, A. G., Kanyshkova, T. G., Mogilnitsky, A. S., Naumov, V. A., Buneva, V. N., Gusev, E. I., Boyko, A. N., Zargarova, T. A., Favorova, T. A., and Nevinsky, G. A. (1998) Biochemistry (Moscow), 63, 1239-1248.
Vlassov, A. V., Andrievskaya, O. A., Kanyshkova, T. G., Baranovsky, A. G., Naumov, V. A., Breusov, A. A., Giege R., Buneva, V. N., and Nevinsky, G. A. (1997) Biochemistry (Moscow), 62, 474-479.
Nevinsky, G. A., Kanyshkova, T. G., Semenov, D. V., Vlassov, A. V., Gal'vita, A. V., and Buneva, V. N. (2000) Appl. Biochem. Biotechnol., 83, 115-129.
Li, Z., Schettino, E. W., Padlan, E. A., Ikematsu, H., and Casali, P. (2000) Eur. J. Immunol., 30, 2015-2026.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nevzorova, T.A., Temnikov, D.A. & Vinter, V.G. Special Features of the DNA-Hydrolyzing Activity of the Antibodies in Systemic Lupus Erythematosus. Biochemistry (Moscow) 68, 1300–1306 (2003). https://doi.org/10.1023/B:BIRY.0000011650.48894.7e
Issue Date:
DOI: https://doi.org/10.1023/B:BIRY.0000011650.48894.7e