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Acridones and Quinacridones: Novel Fluorophores for Fluorescence Lifetime Studies

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Abstract

Two new families of fluorescent probe, acridones and quinacridones, whose fluorescence lifetime can be altered to produce a range of lifetimes from 3 ns to 25 ns are described. Both families of fluorophore have fluorescence lifetimes which are unaffected by pH in the range of 5 to 9 and show a marked resistance to photobleaching. The probes have been modified to allow them to be attached to biomolecules and the labelling of a neuropeptide (substance P) is described. The labelled peptides have the same fluorescence lifetime as the free fluorophore. Quinacridone, with an emission around 550 nm offers a long fluorescence lifetime, photostable alternative to fluorescein.

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  1. Amersham Biosciences, Amersham plc, The Maynard Centre, Forest Farm, Whitchurch, Cardiff, CF14 7YT, United Kingdom

    J. Anthony Smith, Richard M. West & Malcolm Allen

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Smith, J.A., West, R.M. & Allen, M. Acridones and Quinacridones: Novel Fluorophores for Fluorescence Lifetime Studies. Journal of Fluorescence 14, 151–171 (2004). https://doi.org/10.1023/B:JOFL.0000016287.56322.eb

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