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Relationship Between Fecal Calprotectin, Intestinal Inflammation, and Peripheral Blood Neutrophils in Patients with Active Ulcerative Colitis

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Abstract

Active ulcerative colitis (UC) is associated with elevated granulocytes and monocytes/macrophages (GM) which show activation behavior and increased survival time. Further, fecal calprotectin (a stable neutrophil protein) level parallels intestinal inflammation and can predict UC relapse. Since GM are major sources of inflammatory cytokines and chemokines, they are suspected to have roles in the initiation and perpetuation of UC. Our objective was to investigated relationships between peripheral blood (PB) neutrophils, calprotectin, and UC disease activity. Full PB and calprotectin were determined in 69 healthy controls and 31 patients with UC, then 7 randomly selected patients received GM adsorptive apheresis (GMA) with Adacolumn, 10 sessions of 60-min duration each. Patients with UC had higher neutrophil counts (P<0.001), but lower lymphocyte counts (P<0.001) compared with controls. Further, fecal calprotectin levels showed a correlation with UC clinical activity index (CAI; P<0.001) and mucosal inflammation (P<0.001). Following GMA, there were falls in neutrophils (P<0.02), CAI (P<0.02) and calprotectin (P<0.02). In conclusion, GM appear to contribute to intestinal inflammation and UC activity and reduction of these cells by GMA should benefit patients with active UC. Further, the correlations among calprotectin, UC activities, and PB neutrophils should serve as the basis for preemptive actions to control this disease.

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Authors and Affiliations

  1. Department of Endoscopic and Photodynamic Medicine, Hamamatsu University, Handayama, Japan

    Hiroyuki Hanai, Ken Takeuchi & Takayuki Iida

  2. Japan Immunoresearch Laboratories, Takasaki, Japan

    Nobuhito Kashiwagi & Abby R. Saniabadi

  3. Kyoto Prefectural University, Japan

    Nobuhito Kashiwagi, Isao Matsushita & Yoshihiko Sato

  4. Otsuka Tokyo Assay Laboratories, Tokyo, Japan

    Naoki Kasuga & Toshihiro Nakamura

Authors
  1. Hiroyuki Hanai
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  2. Ken Takeuchi
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  3. Takayuki Iida
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  4. Nobuhito Kashiwagi
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  5. Abby R. Saniabadi
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  6. Isao Matsushita
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  7. Yoshihiko Sato
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  8. Naoki Kasuga
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  9. Toshihiro Nakamura
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Hanai, H., Takeuchi, K., Iida, T. et al. Relationship Between Fecal Calprotectin, Intestinal Inflammation, and Peripheral Blood Neutrophils in Patients with Active Ulcerative Colitis. Dig Dis Sci 49, 1438–1443 (2004). https://doi.org/10.1023/B:DDAS.0000042243.47279.87

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  • DOI: https://doi.org/10.1023/B:DDAS.0000042243.47279.87

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