Abstract
A cell density monitor, comprising parts of a liquid chromatography system, was adapted for on-line real-time measurement of bacterial cell density up to 75 g l−1. The device dilutes the sample containing cells and measures the absorbancy at 280 nm. This detector gives an excellent correlation with off-line measurements, good reproducibility, a low limit of detection (3.5 mg l−1), and becomes a low cost alternative for fermentation monitoring in small stirred bioreactors.
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Forcato, D.O., Pécora, R.P. & Kivatinitz, S.C. On-line biomass monitoring in bench-scale stirred bioreactors using parts of a liquid chromatography system. Biotechnology Letters 24, 1999–2003 (2002). https://doi.org/10.1023/A:1021110730741
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DOI: https://doi.org/10.1023/A:1021110730741