Skip to main content

Advertisement

SpringerLink
Log in

An optimized electroporation protocol for transfection of sensitive cell lines using basic laboratory equipment

  • Published:
Biotechnology Techniques

Abstract

Using previously reported protocols, electroporation of 21 very sensitive human cell lines showed poor results with high mortality and low transfection efficiency. Therefore, the influence of several electroporation parameters on transfection success was analyzed. The adjustment of the time constant proved to be most important for optimization of transfection results. Time constant was modulated by changing medium resistance via volume or ionic strength, yielding an average transfection efficiency of 25% and mortality rates below 60%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andreason, GL and Evans, GA (1989). Anal Biochem 180:269–275

    Google Scholar 

  • Baum, C, Forster, P, Hegewisch-Becker, S and Habers, K (1994). Biotechniques 17:1058–1062

    Google Scholar 

  • Chu, G, Hayakawa, H and Berg, P (1987). Nucleic Acids Res 15:1311–1326

    Google Scholar 

  • Gerharz, CD, Hildebrandt, B, Moll, R, Ramp, U, Sarbia, M, Störkel, S, Koldovsky, P and Gabbert, HE (1996). Brit J Cancer 74:1605–1614

    Google Scholar 

  • MacGregor, GR (1992). Optimization of electroporation using reporter genes. In: Guide to electroporation and electrofusion, DC Chang, BM Chassy, JA Saunders and AE Sowers, eds pp. 465–470, San Diego: Academic Press

    Google Scholar 

  • Neumann, E, Schaefer-Ridder, M, Wang, Y and Hofschneider, PH (1982). EMBO J 1:841

    Google Scholar 

  • Potter, H (1992). Protocols for using electroporation to stably or transiently transfect mammalian cells. In: Guide to electroporation and electrofusion, DC Chang, BM Chassy, JA Saunders and AE Sowers, eds pp. 457–461, San Diego: Academic Press

    Google Scholar 

  • Tur-Kaspa, R, Teicher, L, Levine, BJ, Skoultchi, AI and Shafritz, DA (1986). Mol Cell Biol 6:716–718

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Pathology, Heinrich Heine University, Moorenstr. 5, D-40225, Düsseldorf, Germany

    T. Kalinski, K. Jaquet, R. Langen, L. Wang, H.E. Gabbert & C.D. Gerharz

Authors
  1. T. Kalinski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Jaquet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Langen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H.E. Gabbert
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C.D. Gerharz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kalinski, T., Jaquet, K., Langen, R. et al. An optimized electroporation protocol for transfection of sensitive cell lines using basic laboratory equipment. Biotechnology Techniques 11, 717–722 (1997). https://doi.org/10.1023/A:1018432031735

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018432031735

Keywords

Search

Navigation