Skip to main content
SpringerLink
Log in

1H-filtered correlation experiments for assignment and determination of coupling constants in backbone labelled proteins

  • Published:
Journal of Biomolecular NMR Aims and scope Submit manuscript

Abstract

The implementation of [13Cα,13C′,15N,2Hα] labelled amino acids into proteins allows the acquisition of high resolution triple resonance experiments. We present for the first time resonance assignments facilitated by this new labelling strategy. The absence of 1JCα,Cβ couplings enables us to measure 1JCα,C′ scalar and 1DCα,C′ residual dipolar coupling constants using modified HNCA experiments which do not suffer from sensitivity losses characteristic for 13C constant time experiments.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baur, M., Gemmecker, G. and Kessler, H. (1998) J. Magn. Reson., 132,191–196.

    Google Scholar 

  • Coughlin, P.E., Anderson, F.E., Oliver, E.J., Brown, J.M., Homans, S.W., Pollak, S. and Lustbader, J.W. (1999) J. Am. Chem. Soc., 121,11871–11874.

    Google Scholar 

  • Giesen, A.W., Bae, L.C., Barrett, C.L., Chyba, J.A., Chaykovsky, M.M., Cheng, M.-C., Murray, J.H., Oliver, E.J., Sullivan, S.M., Brown, J.M., Dahlquist, F.W. and Homans, S.W. (2001) J. Biomol. NMR.,19,255–260.

    Google Scholar 

  • Grzesiek, S. and Bax, A. (1992) J. Magn. Reson., 99, 201–207.

    Google Scholar 

  • Hitchens, T.K., McCallum, S. and Gordon, R.S. (1999) J. Magn. Reson.,140,281–284.

    Google Scholar 

  • Kupce, E. and Wagner, G.J. (1995) J. Magn. Reson.,B109,329-333.

    Google Scholar 

  • Marion, D., Ikura, M., Tschudin, R. and Bax, A. (1989) J. Magn. Reson., 85,393–399.

    Google Scholar 

  • Matsuo, H., Kupce, E., Li, H. and Wagner, G. (1996) J. Magn. Reson., B113,91–96.

    Google Scholar 

  • McCoy, M.A. (1995) J. Magn. Reson.,B107,270–273.

    Google Scholar 

  • McCoy, M.A. and Mueller, L. (1992) J. Am. Chem. Soc.,114, 2108–2112.

    Google Scholar 

  • Ottiger, M. and Bax, A. (1998a) J. Am. Chem. Soc.,120,12334–12341.

    Google Scholar 

  • Ottiger, M., Delaglio, F. and Bax, A. (1998b) J. Magn. Reson., 131,373–378.

    Google Scholar 

  • Permi, P. and Annila, A. (2001) J. Biomol. NMR,20,127–133.

    Google Scholar 

  • Permi, P., Rosevear, P.R. and Annila, A. (2000) J. Biomol. NMR, 17,43–54.

    Google Scholar 

  • Salzmann, M., Pervushin, K., Wider, G., Senn, H. and Wüthrich, K. (1999) J. Biomol. NMR, 14, 85–88.

    Google Scholar 

  • Santoro, J. and King, G.C. (1992) J. Magn. Reson., 97, 202–207.

    Google Scholar 

  • Seip, S., Balbach, J. and Kessler, H. (1994) J. Magn. Reson., B104,172–179.

    Google Scholar 

  • Sklenar, V., Piotto, M., Leppik, R. and Saudek, V. (1993) J. Magn. Reson., A102, 241–245.

    Google Scholar 

  • Tjandra, N., Grzesiek, S. and Bax, A. (1996) J. Am. Chem. Soc., 118,6264–6272.

    Google Scholar 

  • Tolman, J.R. and Prestegard, J.H. (1996) J. Magn. Reson., B112, 245–252.

    Google Scholar 

  • Venters, A., Farmer II, B.T., Fierke, C.A. and Spicer, L.D. (1996) J. Mol. Biol.,264, 1101–1116.

    Google Scholar 

  • Vuister, G.W. and Bax, A. (1992) J. Magn. Reson., 98, 428–435.

    Google Scholar 

  • Weisemann, R., Rueterjans, H., Schwalbe, H., Schleucher, J., Bermel, W. and Griesinger, C. (1994) J. Biomol. NMR,4, 231–240.

    Google Scholar 

  • Yamazaki, T., Lee, W., Revington, M., Mattiello, D.L., Dahlquist, F.W., Arrowsmith, C.H. and Kay, L.E. (1994) J. Am. Chem. Soc., 116, 6464–6465.

    Google Scholar 

  • Yang, D., Venters, R.A., Muller, G.A., Choy, W.Y. and Kay, L.E (1999) J. Biomol. NMR,14,333–343.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Biochemistry and Molecular Biology, University of Leeds, LS2 9JT , U.K

    Alexander W. Giesen & Steve W. Homans

  2. Martek Biosciences, 6480 Dobbin Road, Columbia, MD, 21045, U.S.A

    Lynnette C. Bae, Christa L. Barrett, Jason A. Chyba, Michael M. Chaykovsky, Minn-Chang Cheng, Jenny H. Murray, Ed J. Oliver, Sarah M. Sullivan & Jonathan Miles Brown

Authors
  1. Alexander W. Giesen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lynnette C. Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christa L. Barrett
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jason A. Chyba
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Michael M. Chaykovsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Minn-Chang Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jenny H. Murray
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ed J. Oliver
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Sarah M. Sullivan
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jonathan Miles Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Steve W. Homans
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Steve W. Homans.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Giesen, A.W., Bae, L.C., Barrett, C.L. et al. 1H-filtered correlation experiments for assignment and determination of coupling constants in backbone labelled proteins. J Biomol NMR 22, 21–26 (2002). https://doi.org/10.1023/A:1013831417615

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1013831417615

Search

Navigation