Abstract
A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport chain, ATP/NADPH, ATP/O2 and PS II/chlorophyll ratios. Sensitivity to a light-chill photoinhibitory treatment was also determined by evaluating the decrease in both maximal photochemical efficiency (Fv/Fm) and in electron transport rate.
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References
Asada K (1994) Production and action of active oxygen species in photosynthetic tissues. In: Foyer CH and Mullineaux PM (eds) Causes of Photoxidative Stress and Amelioration of Defense Systems in Plants, pp 77–104. CRC Press, Boca Raton, Florida
Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol 24: 1–15
Chow WS, Hope AB, Anderson JM (1990) A reassessment of the use of herbicide binding to measure Photosystem II reaction centres in plant thylakoids. Photosynth Res 24: 109–113
Dinkins RD, Bandaranayake H, Green BR and Griffiths AJF (1994) A nuclear photosynthetic electron transport mutant of Arabidopsis thaliana with altered expression of the chloroplast petA gene. Curr Genet 25: 282–288
Forti G (1987) Energy conversion in higher plants and algae. In: Amesez J (ed) Photosynthesis: New Comprehensive Biochemistry, Vol 15, pp 1–20. Elsevier, Amsterdam
Forti G and Ehrenheim AM (1993) The role of ascorbic acid in photosynthetic electron transport. Biochim Biophys Acta 1183: 408–412
Forti G and Elli G (1995) The Function of Ascorbic Acid in Photosynthetic Phosphorylation. Plant Physiol 109: 1207–1211
Haertel H, Lokstein H, Doermann P, Grimm B and Benning C (1997) Changes in the Composition of the Photosynthetic Apparatus in the Galactolipid-Deficient dgd1 Muntant of Arabidopsis thaliana. Plant Physiol 115: 1175–1184
Haldrup A, Naver H and Scheller HV (1999) The interaction between plasocyanin and photosystem I is inefficient in transgenic Arabidopsis plant lacking the PS I-N subunit of Photosystem I. Plant J 17(6): 689–698
Leegood RC and Walker DA (1979) Isolation of Protoplasts and Chloroplasts from Flag Leaves of Triticum aestivum L. Plant Physiol 63: 1212–1214
Meurer J, Berger A and Westhoff P (1996) A Nuclear Mutant of Arabidopsis with Impaired Stability on Distinct Transcripts of the Plastid psbB, psbD/C, ndhH, and ndhC Operons. Plant Cell 8: 1193–1207
McCourt P, Kunst L, Browse J and Somerville CR (1987) The effects of reduced amounts of lipid unsaturation on chloroplast ultrastructure and photosynthesis in a mutant of Arabidopsis. Plant Physiol 84: 353–360
Miyake C and Asada K (1992) Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radical in thylakoids. Plant Cell Physiol 33: 541–553
Norén H, Svensson P and Andersson B (1999) Auxiliary photosynthetic functions of Arabidopsis thaliana — studies in vitro and in vivo. Biosci Rep 19(5): 499–509
Rock C, Bowlby NR, Hoffmann-Benning S and Zeevaart AD (1992) The aba mutant of Arabidopsis thaliana (L.) Heynh. has reduced chlorophyll fluorescence yields and reduced thylakoid stacking. Plant Physiol 100: 1796–1801
Somerville CR, Somerville SC, Ogren WL (1981) Isolation of photosynthetically active protoplasts and chloroplasts from Arabidopsis thaliana. Plant Sci Lett 21: 89–96
Tarantino D, Vianelli A, Carraro L and Soave C (1999) A nuclear mutant of Arabidopsis thaliana selected for enhanced sensitivity to light-chill stress in PS II electron transport activity. Physiol Plant 107: 361–371
Trebst A (1978) Organisation of the photosynthetic electron transport system of chloroplast in the thylakoid membrane. In: Schaefer GG and Klingenberg M (eds) Energy Conservation in Biological Membranes, pp 84–95. Springer-Verlag, Berlin/Heidelberg
Walters R and Horton P (1994) Acclimation of Arabidopsis thaliana to the light environment: Changes in composition of the photosynthetic apparatus. Planta 195: 248–256
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Casazza, A.P., Tarantino, D. & Soave, C. Preparation and functional characterization of thylakoids from Arabidopsis thaliana. Photosynthesis Research 68, 175–180 (2001). https://doi.org/10.1023/A:1011818021875
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DOI: https://doi.org/10.1023/A:1011818021875