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Acquisition of Multiple Virulence/Avirulence Determinants by Potato Virus X (PVX) has Occurred Through Convergent Evolution Rather than Through Recombination

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Abstract

Resistance to potato virus X (PVX) is determined by the product of a host resistance gene and a viral determinant specifying either virulence (resistance-breaking ability) or avirulence (resistance sensitivity). The viral coat protein is the determinant of resistance mediated by the host Nx gene while the 25 kDa movement protein is the determinant of Nb-mediated resistance. Group 1 and group 4 strains of PVX are avirulent or virulent respectively for both these determinants while group 2 and group 3 strains are virulent for one but avirulent for the other determinant. There are two alternative evolutionary mechanisms by which the various strain groups might have evolved: either by recombination between strains carrying virulence (or avirulence) determinants that evolved once only, or alternatively, by independent evolution of at least one virulence (or avirulence) determinant in distinct phylogenetic branches. These alternative hypotheses were investigated by (i) determining the complete genomic sequence of a group 1 and a group 4 strain and (ii) comparing the completely sequenced genomes of six isolates representative of the four strain groups. The analysis revealed the same phylogeny for all five PVX genes. Thus, there is no evidence that the PVX strain groups evolved by recombination.

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References

  1. Huisman M.J., Linthorst H.J.M., Bol J.F., and Cornellissen B.J.C., J Gen Virol 69, 1789-1798, 1988.

    Google Scholar 

  2. Beck D.L., Guilford P.J., Voot D.M., Andersen M.T., and Forster R.L., Virology 183, 695-702, 1991.

    Google Scholar 

  3. Angell S.M., Davies C., and Baulcombe D.C., Virology 215, 197-201, 1996.

    Google Scholar 

  4. Cockerham G., Heredity 25, 309-348, 1970.

    Google Scholar 

  5. Solomon R.M., Heredity 55, 135-138, 1985.

    Google Scholar 

  6. Kohm B.A., Goulden M.G., Gilbert J.E., Kavanagh T.A., and Baulcombe D.C., The Plant Cell 5, 913-920, 1993.

    Google Scholar 

  7. Baulcombe D.C., Kohm B., Goulden M.G., Santa Cruz S., Forsyth A., Chapman S., and Kavanagh T., in Bills D., Owens R., Baker C.J., Hutcheson S., Straney D., Fischoff D., and Shah D. (eds). Proceedings of the Fifth International Symposium on Biotechnology and Plant Protection. University of Maryland, World Scientific Publishing Co., New Jersey, 1993, pp. 101-110.

    Google Scholar 

  8. Greenberg J.T., Guo A., Klessig D.F., and Ausubel F.M., Cell 77, 551-563, 1994.

    Google Scholar 

  9. Malcuit I.M., Marano M.R., Kavanagh T.A., De Jong W., Forsyth A., and Baulcombe D.C., Mol Plant-Microbe Interact 12, 536-543, 1999.

    Google Scholar 

  10. Kavanagh T., Goulden M., Santa Cruz S., Chapman S., Barker I., and Baulcombe D.C., Virology 189, 609-617, 1992.

    Google Scholar 

  11. Santa Cruz S., and Baulcombe D.C., Molec Plant-Microbe Interact 6, 707-714, 1993.

    Google Scholar 

  12. Bendahmane A., Kohm B.A., Dedi C., and Baulcombe D.C., Plant J 8, 933-941, 1995.

    Google Scholar 

  13. Cockerham G., in Proceedings of the Second Conference on Potato Virus Diseases. Lisse-Wageningen, 1954, pp. 89-92.

  14. Moreira A., Jones R.A.C., and Fribourg C.E., Ann Appl Biol 95, 93-103, 1980.

    Google Scholar 

  15. Simon A.E. and Bujarski J.J., Ann Rev Phytopathol 32, 337-362, 1994.

    Google Scholar 

  16. Holland J., Spindler K., Horodyski F., Grabau E., Nichol S., and VandePol S., Science 215, 1577-1585, 1982.

    Google Scholar 

  17. Jones R.A.C., Plant Pathol 31, 325-331, 1982.

    Google Scholar 

  18. Jones R.A.C., Plant Pathol 34, 182-189, 1985.

    Google Scholar 

  19. Orman B.E., Celnik R.M., Mandel A.M., Torres H.N., and Mentaberry A.N., Virus Res 16, 293-306, 1990.

    Google Scholar 

  20. Skryabin K.G., Kraev A.S., Morozov S.Y., Rozanov M.N., Chernov B.K., Lukasheva L.I., and Atabekov J.G., Nucleic Acids Res 16, 10929-10930, 1988.

    Google Scholar 

  21. Querci M., Van der Vlugt R.A.A., Goldbach R.W., and Salazar L.F., J Gen Virol 74, 2251-2255, 1993.

    Google Scholar 

  22. Goulden M.G., Kohm B., Santa Cruz S., Kavanagh T.A., and Baulcombe D.C., Virology 197, 293-302, 1993.

    Google Scholar 

  23. Morozov S.Y., Zakhariev V.M., Chernov B.K., Prasolov V.S., Kozlov I.V., Atabekov J.G., and Skryabin K.G., Dokl Akad Nauk Sssr 271, 211-215, 1983.

    Google Scholar 

  24. Santa Cruz S. and Baulcombe D.C., J Gen Virol 76, 2057-2061, 1995.

    Google Scholar 

  25. Sambrook J., Fritsch E.F., and Maniatis T., ``Molecular Cloning: A laboratory Manual'', 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, 1989.

    Google Scholar 

  26. Murphy G. and Kavanagh T., Nucleic Acids Res 16, 5198, 1988.

    Google Scholar 

  27. Meshi T., Ishikawa M., Takamatsu N., Ohto T., and Okada Y., FEBS Lett 162, 282-285, 1983.

    Google Scholar 

  28. Zucker M., Science 244, 48-52, 1989.

    Google Scholar 

  29. Jaeger J.A., Turner D.H., and Zucker M., Proc Natl Acad Sci USA 86, 7706-7710, 1989.

    Google Scholar 

  30. Thompson J.D., Higgins D.G., and Gibson T.J., Nucleic Acids Res 22, 4673-4680, 1994.

    Google Scholar 

  31. Saitou N. and Nei M., Mol Biol Evol 4, 406-425, 1987.

    Google Scholar 

  32. Kimura M., The Neutral Theory of Molecular Evolution. Cambridge University Press, Cambridge, England, 1983.

    Google Scholar 

  33. Felsenstein J., Evolution 39, 783-791, 1985.

    Google Scholar 

  34. Felsenstein J., Cladistics 5, 164-166, 1989.

    Google Scholar 

  35. Lai M.M.C., Microbiological Reviews 56, 61-79, 1992.

    Google Scholar 

  36. Figlerowitz M., Nagy P.D., and Bujarski J.J., Proc Natl Acad Sci USA 94, 2073-2078, 1997.

    Google Scholar 

  37. Fraile A., Alonso-Prados J.L., Aranda M.A., Bernal J.J., Malpica J.M., and Garcia-Arenal F., Virology 71, 934-940, 1997.

    Google Scholar 

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Malcuit, I., de Jong, W., Baulcombe, D.C. et al. Acquisition of Multiple Virulence/Avirulence Determinants by Potato Virus X (PVX) has Occurred Through Convergent Evolution Rather than Through Recombination. Virus Genes 20, 165–172 (2000). https://doi.org/10.1023/A:1008178800366

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