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Correlation Between Stellate Cell Activation and Serum Fibrosis Markers in Choline-Deficient l-Amino Acid-Defined Diet-Induced Rat Liver Fibrosis

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Abstract

The aim of this study was to investigate the association of stellate cell activation with serum fibrosis markers in a rat model of hepatic fibrosis prepared using a choline-deficient l-amino acid (CDAA) defined diet. CDAA diet administration resulted in increased liver hydroxyproline contents in a time-dependent manner with activated stellate cells, expressing α-smooth muscle actin (α-SMA) as well as increased serum concentrations of amino-terminal procollagen type III peptide (PIIIP) and the 7S fragment of type IV collagen. Hydroxyproline content of the liver showed a closer correlation with the serum 7S (r = 0.75, P < 0.01) concentration than with the serum PIIIP (r = 0.51, P < 0.01) concentration. The percent area of α-SMA-positive cells showed stronger correlation with the serum PIIIP concentration (r = 0.85, P < 0.01) than with the 7S concentration (r = 0.50, P < 0.01). These results indicate that the serum PIIIP concentration reflects the activity of fibrogenesis, while the serum 7S concentration reflects the accumulation of collagen fibers in the liver.

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Authors and Affiliations

  1. First Department of Internal Medicine, School of Medicine, Yamaguchi University, Ube, Yamaguchi Prefecture, 755-8505, Japan

    Koji Hironaka, Isao Sakaida, Koichi Uchida & Kiwamu Okita

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  1. Koji Hironaka
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  2. Isao Sakaida
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  3. Koichi Uchida
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  4. Kiwamu Okita
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Hironaka, K., Sakaida, I., Uchida, K. et al. Correlation Between Stellate Cell Activation and Serum Fibrosis Markers in Choline-Deficient l-Amino Acid-Defined Diet-Induced Rat Liver Fibrosis. Dig Dis Sci 45, 1935–1943 (2000). https://doi.org/10.1023/A:1005556328028

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