Abstract
We previously reported that application of plant growth-promoting rhizobacteria (PGPR) increased soybean growth and development and, specifically, increased nodulation and nitrogen fixation over a range of root zone temperatures (RZTs) in controlled environment studies. In order to expand on the previous studies, field experiments were conducted on two adjacent sites, one fumigated with methyl bromide and one nonfumigated, in 1994. Two experiments were conducted at each site, one involving combinations of two soybean cultivars and two PGPR strains, the other involving the same factors, but also in combination with two strains Bradyrhizobium japonicum. Soybean grain yield and protein yield were measured. The results of these experiments indicated that co-inoculation of soybean with B. japonicum and Serratia liquefaciens 2-68 or Serratia proteamaculans 1-102 increased soybean grain yield, protein yield, and total plant protein production, compared to the nontreated controls, in an area with low spring soil temperatures. Interactions existed between PGPR application and soybean cultivar, suggesting that PGPRs applied to cultivars with higher yield potentials were more effective. PGPRs applied to the rhizosphere without addition of B. japonicum also increased only leaf area and seed number at the fumigated site. Overall, inoculation of soybean plants with PGPRs in the presence of B. japonicum increased soybean grain yield, grain protein yield, and total plant protein production under short season conditions.
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References
Alagawadi A R and Gaur A C 1988 Associative effect of Rhizobiumand phosphate-solubilising bacteria on the yield and nutrient uptake of chickpea. Plant Soil 105, 241-246.
Bbhuvaneswari T V, Goodman R N and Bauer W D 1980 Early events in the infection of soybean (Glycine maxL. Merr.) by Rhizobium japonicum. I. Location of infectible root cells. Plant Physiol. 66, 1027-1031.
Burr T J and Caesar A 1984 Beneficial plant bacteria. Crit. Rev. Plant Sci. 2, 1-20.
Davison J 1988 Plant beneficial bacteria. Biotechnology 6, 282-286.
De-Ming L I and Alexander M 1988 Co-inoculation with antibiotic producing bacteria to increase colonization and nodulation by rhizobia. Plant Soil 108, 211–219.
Fehr W R, Caviness C E, Burmood D T and Pennington J S 1971 Stages of development descriptions for soybeans, Glycine max(L.) Merrill. Crop Sci. 11, 929-930.
Gaskins M H, Albrecht S L and Hubbel D H 1985 Rhizosphere bacteria and their use to increase productivity: a review. Agric. Ecosys. Environ. 12, 99-116.
Grimes H D and Mount MS 1984 Influence of Pseudomonas putidaon nodulation of Phaseolus vulgaris. Soil Biol. Biochem. 16, 27-30.
Hume D J and Shelp B J 1990 Superior performance of the hup-Bradyrhizobium japonicumstrain 532C in Ontario soybean field trials. Can. J. Plant Sci. 70, 661–666.
Iruthayathas E E, Gunasekaran S and Vlassak K 1983 Effect of combined inoculation of Azospirillumand Rhizobiumon nodulation and nitrogen fixation of winged bean and soybean. Sci. Hortic. 20, 231-240.
Kapulnik Y 1991 Plant-growth promoting rhizobacteria. InPlant Roots The Hidden Half. Eds. Y Waisel, A Eshel and U Kafkafi. pp 719-729. Marcel Dekker, New York.
Kloepper J W, Schroth M N and Miller T D 1980a Effects of rhizosphere colonization by plant growth promoting rhizobacteria on potato plant development and yield. Phytopathology 70, 1078- 1082.
Kloepper JW, Leong L, Teintze M and Schroth MN 1980b Enhanced plant growth by siderophores produced by PGPR. Nature 268, 885-886.
Lie D M and Alexander A 1988 Co-inoculation with antibioticproducing bacteria to increase colonization and nodulation by rhizobia. Plant Soil 108, 211-219.
Lifshitz R, Kloepper JW and Kozlowski M 1987 Growth promotion of canola (rapeseed) seedlings by a strain of Pseudomonas putidaunder gnotobiotics conditions. Can. J. Microbiol. 33, 390-395.
Polonenko D R, Scher F M, Kloepper JW, Singleton C A, Laliberte M and Zaleska I 1987 Effects of root colonizing bacteria on nodulation of soybean roots by Bradyrhizobium japonicum. Can. J. Microbiol. 33, 498-503.
Rai R 1983 Efficacy of associative N2-fixation by streptomycinresistant mutants of Azospirillum brasilensewith genotypes of chick pea Rhizobiumstrains. J. Agric. Sci. 100, 75-80.
SAS Institute Inc 1988 SAS User's Guide. Statistical Analysis Institute Inc., Cary, North Carolina.
Sneh B, Agami O and Baker R 1985 Biological control of Fusariumwilt in carnation with Serratia liquefaciensand Hafnia alveiisolated from rhizosphere of carnation. Phytopathol. Z. 113, 271- 276.
Sprent J I 1979 The Biology of Nitrogen Fixing Organisms. McGraw-Hill Book Company Ltd., New York. 196 p.
Steel R G D and Torrie J H 1980 Principles and Procedures of Statistics: a Biometric Approach. McGraw-Hill, New York.
Summerfield R J and Wien H C 1982 Effects of photoperiod and air temperature on growth and yield of economic legumes. InAdvances in Legume Science. Eds. R J Summerfield and A H Bunting. pp 17-36. Royal Botanic Gardens, Surrey.
Verma D P S, Fortin M G, Stanely J, Mauro V P, Purohit S and Morrison N 1986 Noduilins and nodulin genes of Glycine maxa perspective. Plant Mol. Biol. 7, 51-61.
Vincent J M 1970 A Manual for the Practical Study of Root Nodule Bacteria. Blackwell Scientific Publication, Oxford.
Yahalom E, Okon Y and Dovrat A 1987 Azospirilliumeffects on susceptility to Rhizobiumnodulation and on nitrogen fixation of several forage legumes. Can. J. Microbiol. 33, 510-514.
Zhang F and Smith D L 1995a Preincubation of Bradyrhizobium japonicunwith genistein accelerates nodule development of soybean (Glycine max(L.) Merr) at suboptimal root zone temperatures. Plant Physiol. 108, 961-968.
Zhang F, Hamel C, Kianmehr H and Smith D L 1995b Root-zone temperature and soybean (Glycine max(L.) Merr) vesiculararbuscular mycorrhizae: Development and interactions with the nitrogen fixation symbiosis. Environ. Exp. Bot. 35, 287-298.
Zhang F, Dashti N, Hynes H and Smith D L 1996a Plant growth promoting rhizobacteria and soybean (Glycine maxL. Merr.) nodulation and nitrogen fixation at suboptimal root zone temperatures. Ann. Bot. 77, 453-459.
Zhang F and Smith D L 1996b Inoculation of soybean (Glycine max(L.) Merr). with genistein-preincubated Bradyrhizobium japonicunor genistein directly applied into soil increases soybean protein and drymatter yield under short season conditions. Plant Soil 179, 233-241.
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Dashti, N., Zhang, F., Hynes, R. et al. Application of plant growth-promoting rhizobacteria to soybean (Glycine max [L.] Merr.) increases protein and dry matter yield under short-season conditions. Plant and Soil 188, 33–41 (1997). https://doi.org/10.1023/A:1004295827311
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DOI: https://doi.org/10.1023/A:1004295827311