Skip to main content
Log in

Biomass characterization of Azolla filiculoides grown in natural ecosystems and wastewater

  • Published:
Hydrobiologia Aims and scope Submit manuscript

Abstract

The aquatic pteridophyte Azolla, a small-leaf floating plant, which lives in symbiosis with a nitrogen fixing cyanobacteria, Anabaena azollae, was widespread throughout water channels and hydrographic basins of Portugal. Azolla is also the aim of a study for its utilization as a biofilter for wastewater purification, namely for phosphorus removal (± 36%). The goal of this work is to compare the growth characteristics and biomass composition of this water fern in natural ecosystems with those obtained in some wastewaters. Plant growth rate (0.107± 0.037 d-1) and productivity (5.8 g dw m-2 d-1) suggest that Azolla can grow well in partially treated domestic wastewater, but not in diluted pig wastes. This fact, associated to its biomass composition, namely in phosphorus content (1.38 ±0.20%), increase the possibility of this plant being used to improve wastewater discharge quality. It may also be possible to use the biomass as a biofertiliser or as a feed supplement for aquatic and terrestrial animals due to its protein, crude fiber and mineral content.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • APHA, AWWA, APCF, 1992. Standard methods for the examination of water and wastewater. 20th American Public Health Association, Washington.

    Google Scholar 

  • AOAC, 1990. Official methods of analysis, 15th edn., Vol II.

  • Barber, S. A., 1995. Soil nutrient bioavailability, 2nd edn., John Wiley & Sons, Inc., New York: 157–179.

    Google Scholar 

  • Bishop, P. L. & T. T. Eighmy, 1989. Aquatic wastewater treatment using Elodea nuttallii. Journal WPCF 61: 641–648.

    Google Scholar 

  • C.E.I.P., 1980. Tables de composition des matiers premiers destinées à alimentation animale. 1 - valeurs chimiques.

  • DeBusk, T. A. & J. H. Ryther, 1987. Biomass production and yields of aquatic plants. In Reddy, K. R. & W. H. Smith (eds), Aquatic Plants for Water Treatment and Research Recovery. Florida: 579–598.

  • DeBusk, T. A., K. R. Reddy, T. D. Hayes & B. R. Schwegler, 1989. Performance of a pilot-scale water hyacinth-based secondary treatment system. Journal WPCF 61: 1217–1224.

    Google Scholar 

  • Costa, M. L., F. Carrapiço & M. C. R. Santos, 1994. Biomass and growth characterization of Azolla filiculoides in natural and artificial environments. In Hegazi, N. A., M. Fayez & M. Monib (eds), Nitrogen Fixation with Non-Legumes. The American University in Cairo Press: 455–461.

  • Costa, M. L., F. Carrapiço & M. C. R. Santos, 1996. Contribuição para o estudo da utilização de Azolla no tratamento de águas residuais domésticas. In Borrego, C., C. Coelho, L. Arroja, C. Bóia & E. Figueiredo (eds), Proceedings 5a Conferência Nacional Sobre a Qualidade do Ambiente 2. Aveiro, Portugal: 1945–1954.

    Google Scholar 

  • Fisher, J. P., 1988. Wastewater treatment using aquatic plants. In (eds),Alternative Waste Treatment Systems, Rao Bhamidimarri, Elsevier Applied Science, Palmerston North, New Zealand: 34–44.

    Google Scholar 

  • Kitoh, S., N. Shiomi & E. Uheda, 1993. The growth and nitrogen fixation of Azolla filliculoides Lam. in polluted water. Aquat. Bot. 46:129–139.

    Google Scholar 

  • Kumar, P. & R. J. Garde, 1989. Potentials of water hyacinth for sewage treatment. Journal WPCF 61: 1702–1706.

    Google Scholar 

  • Kumarasinghe, K. S. & D. L. Eskew, 1993. Isotopic studies of Azolla and nitrogen fertilization of rice, Kluwer Academic Publishers, London: 42–54.

    Google Scholar 

  • Lumpkin, T. A. & D. L. Plucknett, 1980. Azolla: botany, physiology and use as green manure. Econom. Bot. 34: 111–153.

    Google Scholar 

  • M.A.D.R.P., 1997. Código de boas práticas agrícolas para a protecção da água contra a poluição com nitratos de origem agrícola, Ministério da Agricultura, do Desenvolvimento Rural e das Pescas, Lisboa.

    Google Scholar 

  • Martins, M. I. F. M., 1996. Maize nitrogen fertilisation in fluvisoles of the lower Mondego valley - Field trials, In Van Cleemput, O. G. Hofman & A. Vermossen (eds), Progress in Nitrogen Cycling Studies, Kluwer Academic Publishers, London: 223–229.

    Google Scholar 

  • Mian, M. H. & A. K. M. Azmal, 1989. The response of Azolla pinnata R. Brown to the split application of phosphorus and the transfer of assimilated phosphorus to flooded rice plants, Plant Soil 119: 211–216.

    Google Scholar 

  • Moorhead, K. K. & K. R. Reddy, 1987. Nitrogen cycling in an integrated 'biomass for energy' system. In Reddy, K. R. & W. H. Smith (eds), Aquatic Plants for Water Treatment and Research Recovery, Florida: 535–542.

  • Oron, G., 1990. Economic considerations in wastewater treatment with duckweed for effluent and nitrogen renovation. Journal WPCF 62: 692–696.

    Google Scholar 

  • Reddy, K. R. & W. F. DeBusk, 1985. Growth characteristics of aquatic macrophytes cultured in nutrient-enriched water: II. Azolla, duckweed and Salvinia, Econom. Bot. 39: 200–208.

    Google Scholar 

  • S.A.P.E.C., 1989. Manual de adubação, 2nd. edn., SAPEC - Adubos Agro, S.A.

    Google Scholar 

  • Vicenzini, M., M. C. Margueri & C. Sili, 1985. Outdoor mass culture of Azolla spp.: yields and efficiency of nitrogen fixation. Plant Soil 86: 57–67.

    Google Scholar 

  • Watanabe, I. & C. M. Ramirez, 1984. Relationship between soil phosphorus availability and Azolla growth. Soil Sci. Plant Nutr. 30: 595–598.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Costa, M.L., Santos, M.C. & Carrapiço, F. Biomass characterization of Azolla filiculoides grown in natural ecosystems and wastewater . Hydrobiologia 415, 323–327 (1999). https://doi.org/10.1023/A:1003824426183

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1003824426183

Navigation