Received May 5, 2006

Abstract:

Various hydroxylated additives were added to antibody print buffers at different concentrations to stabilize printed antibodies during normal array spot desiccation on commercial polymer-coated microarray slides. Polyvinyl alcohol addition to print buffers produced the most regular spot morphologies, homogeneous intra-spot antibody distribution, uniform fluorescence intensity, and improved analyte capture activity, maintained up to 1 month at 4 C for capturing model analytes, anti-human IL-1, IL-4, and TNF, on these microarraying slides.

Keywords: microarray antibody surface chemistry immobilization assay diagnostics shelf life

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