Web Release Date: July 15,
Production of Ascorbyl Palmitate by Surfactant-Coated Lipase in Organic Media
and
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, and Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan
Received for review January 11, 2006. Revised manuscript received June 8, 2006. Accepted June 13, 2006.
Abstract:
The surface of a lipase from Burkholderia cepacia was coated with a nonionic surfactant, propylene
glycol monostearate, and was used as a biocatalyst in the production of ascorbic acid in tert-butyl
alcohol. The influence of various factors such as the type of surfactant, the pH of the buffer used for
coating, the amount of surfactant in the coating, the organic solvent, and the temperature and molar
ratio of the substrates used in the reaction on the conversion of ascorbyl palmitate were studied.
After 24 h of reaction at 50 
C, a conversion of 47% was obtained using an ascorbic acid to palmitic
acid molar ratio of 1:6. The native lipase showed only 6% conversion.
Keywords: Acylation; antioxidant; ascorbyl palmitate; surfactant-coated lipase
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