Web Release Date: September 28,
Electrochemical Detection of Glutathione Using Redox Indicators
Department of Chemistry and Center for Biomodular Multiscale Systems, Louisiana State University, 232 Choppin Hall, Baton Rouge, Louisiana 70803-1804
Received for review August 4, 2006. Accepted August 21, 2006.
Abstract:
The nucleophilic addition of the aminothiols homocysteine
(HCY), cysteine (CYS), and glutathione (GSH) to the
electrogenerated quinone of fluorone black (1) via the
ECE mechanism is reported. It is demonstrated that 1
selectively reacts with GSH to form the bis-GSH adduct,
1-(GSH)2, while only the monothiol adducts were generated in the presence of HCY and CYS (1-HCY and
1-CYS, respectively). The more anodic Epa of 1-(GSH)2
relative to 1 and 1-GSH (
Epa ~ +0.14) is the voltammetric signature that allows the discrimination of GSH
from HCY and CYS. It is also shown that the presence of
structurally similar aminothiols-HCY and CYS-posed no
interference to the signature voltammetric response of
1-(GSH)2.
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