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Effects of kisspeptin on the maturation of human ovarian primordial follicles in vitro

Published online by Cambridge University Press:  15 December 2023

Fatemeh Rezaei-Tazangi Open the ORCID record for Fatemeh Rezaei-Tazangi [Opens in a new window] ,
Leila Kooshesh ,
Ali Tayyebiazar ,
Neda Taghizabet ,
Anahita Tavakoli Open the ORCID record for Anahita Tavakoli [Opens in a new window] ,
Ashraf Hassanpour ,
Fereshteh Aliakbari ,
Ebrahim Kharazinejad  and
Ali-Mohammad Sharifi
Fatemeh Rezaei-Tazangi
Affiliation:
Department of Anatomy, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
Leila Kooshesh
Affiliation:
Department of Genetics, Fars Academic Center for Education, Culture and Research, ACECR, Shiraz, Iran
Ali Tayyebiazar
Affiliation:
Clinical Research Development Unit of Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, I.R. Iran
Neda Taghizabet
Affiliation:
Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Anahita Tavakoli
Affiliation:
Department of Biology, Faculty of Science, Arak University, Arak, Iran
Ashraf Hassanpour
Affiliation:
Anatomical Department, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
Fereshteh Aliakbari*
Affiliation:
Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Ebrahim Kharazinejad
Affiliation:
Department of Anatomy, Faculty of Medicine, Abadan University of Medical Sciences, Abadan, Iran
Ali-Mohammad Sharifi
Affiliation:
Clinical Research Development Center, Shahid Modarres Educational Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
*
Corresponding author: Fereshteh Aliakbari; Email: fereshtehaliakbari60@gmail.com
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Summary

At this time, with advances in medical science, many cancers and chronic diseases are treatable, but one of their side effects is infertility. Some women also want to delay pregnancy for personal reasons. There has been some evidence that kisspeptin activates broad signals by binding to its receptor, suggesting that the role of kisspeptin in direct control of ovarian function includes follicle growth and steroid production. In this study, the effect of kisspeptin on improving the quality and results for human ovarian follicles was investigated. A section of ovary was removed laparoscopically from women between 20 and 35 years of age (n = 12). Pieces were divided randomly into two groups, control and treatment (with 1 μM kisspeptin). Real-time PCR was performed for GDF9, BMP15 and mTOR gene expression assessments. Western blotting was carried out to measure AKT and FOXO3a protein expression. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey’s test; means were considered significantly different at a P-value < 0.05. During treatment with the kisspeptin group, maturity genes are expressed. Therefore, kisspeptin is an effective substance to improve the quality of the human ovarian medium as it increases the maturity of follicles.

Keywords

BMP15Follicle maturationGDF9Human ovarian folliclesKisspeptin
Type
Research Article
Information
Zygote , Volume 32 , Issue 1 , February 2024 , pp. 66 - 70
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Copyright
© The Author(s), 2023. Published by Cambridge University Press

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Footnotes

Leila Kooshesh and Ali Tayyebiazar contributed equally to this work.

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