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Molecular investigation of the Pfmdr1 gene of Plasmodium falciparum isolates in Henan Province imported from Africa

Published online by Cambridge University Press:  27 September 2018

Chengyun Yang Open the ORCID record for Chengyun Yang [Opens in a new window] ,
Ruimin Zhou ,
Ying Liu ,
Suhua Li ,
Dan Qian ,
Yuling Zhao ,
Hongwei Zhang  and
Bianli Xu
Chengyun Yang
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Ruimin Zhou
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Ying Liu
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Suhua Li
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Dan Qian
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Yuling Zhao
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Hongwei Zhang*
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
Bianli Xu
Affiliation:
Department of Parasite Disease Control and Prevention, Henan Province Center for Disease Control and Prevention, Zhengzhou, Henan, China
*
Author for correspondence: Hongwei Zhang, E-mail: zhwei69@163.com
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Abstract

Efficacious antimalarial drugs are important for malaria control and elimination, and continuous monitoring of their efficacy is essential. The prevalence and distribution of Pfmdr1 were evaluated in African migrant workers in Henan Province. Among 632 isolates, 13 haplotypes were identified, NYSND (39.87%, 252/632), YYSND (2.85%, 18/632), NFSND (31.01%, 196/632), NYSNY (0.47%, 3/632), YFSND (13.77%, 87/632), NFSNY (0.32%, 2/632), YYSNY (2.06%, 13/632), YFSNY (0.16%, 1/632), N/Y YSND (1.90%, 12/632), N Y/F SND (6.17%, 39/632), N/Y Y/F SND (0.47%, 3/632), YYSN D/Y (0.16%, 1/632) and N/Y FSND (0.79%, 5/632). The highest frequency of NYSND was observed in individuals from North Africa (63.64%, 7/11), followed by South Africa (61.33%, 111/181), Central Africa (33.33%, 56/168), West Africa (28.94%, 68/235) and East Africa (27.03%, 10/37) (χ2 = 54.605, P < 0.05). The highest frequency of NFSND was observed in East Africa (48.65%, 18/37), followed by West Africa (39.14%, 92/235), Central Africa (26.79%, 45/168), South Africa (22.65%, 41/181) and North Africa (9.09%, 1/11) (χ2 = 22.368 P < 0.05). The mutant prevalence of codons 86 and 184 decreased. These data may provide complementary information on antimalarial resistance that may be utilized in the development of a treatment regimen for Henan Province.

Keywords

Africaantimalarial drugsHenan ProvincemalariaPfmdr1Plasmodium falciparum
Type
Research Article
Information
Parasitology , Volume 146 , Issue 3 , March 2019 , pp. 372 - 379
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Copyright
Copyright © Cambridge University Press 2018 

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