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Role of heat-labile serum factor or host complement in the inhibition of Plasmodium falciparum sporogonic stages in Anopheles stephensi by gametocyte carriers' serological factors

Published online by Cambridge University Press:  24 July 2007

L. C. GOUAGNA ,
M. VAN DER KOLK ,
W. ROEFFEN ,
J.-P. VERHAVE ,
W. ELING ,
R. SAUERWEIN  and
C. BOUDIN
L. C. GOUAGNA*
Affiliation:
Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Laboratoire de Recherche sur le Paludisme, BP 288, Yaoundé, Cameroon Unité de Recherche 016 – Antenne de l'Institut de Recherche pour le Développement (IRD), BP 171 Bobo Dioulasso, Burkina Faso
M. VAN DER KOLK
Affiliation:
Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Laboratoire de Recherche sur le Paludisme, BP 288, Yaoundé, Cameroon Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, CRCN 395, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
W. ROEFFEN
Affiliation:
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, CRCN 395, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
J.-P. VERHAVE
Affiliation:
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, CRCN 395, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
W. ELING
Affiliation:
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, CRCN 395, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
R. SAUERWEIN
Affiliation:
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, CRCN 395, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
C. BOUDIN
Affiliation:
Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Laboratoire de Recherche sur le Paludisme, BP 288, Yaoundé, Cameroon Unité de Recherche 077 – Institut de Recherche pour le Développement (IRD), Dakar
*
Corresponding author: Unité de Recherche 016 – Institut de Recherche pour le Développement, Antenne IRD. 01 BP 171 Bobo Dioulasso, Burkina Faso. Tel: +226 974987. Fax: +226 2097 0942. E-mail: Louis-Clement.Gouagna@ird.bf
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Summary

This study investigated the significance of serum complement on transmission-reducing activity (TRA) of field sera from 24 infected Plasmodium falciparum gametocyte carriers (from Cameroon) against cultured NF54 P. falciparum. Laboratory-reared Anopheles stephensi were given infectious blood meals prepared either with sera from naïve Dutch donor (AB type) or pair-matched field serum samples, both with and without active complement. TRA of serum factors and host complement on mosquito infection rate and oocyst intensity were divided into the various components involved in the early stages of sporogony. The majority (>80%) of sera tested showed positive antibody titres to Pfs230, the relevant complement-dependent target of transmission-reducing mechanisms. Regardless of the presence of active complement, bloodmeals with field sera exhibited significantly lower infection rates and oocyst intensity than the control group. Serological reactivity in Capture-ELISA against Pfs230 was significantly correlated with the reduction of parasite infectivity. Contrary to our expectation, the presence of active complement in the mosquito bloodmeal did not increase parasite losses and therefore the magnitude of transmission reduction by individual immune sera. Our findings on P. falciparum are consistent with previous studies on animal hosts of Plasmodium, indicating that early P. falciparum sporogonic stages may be insensitive to the antibody-dependent pathways of complement in human serum.

Keywords

Plasmodium falciparumgametocyte carrierscomplementAnopheles stephensisporogonytransmission-reducing immunity
Type
Research Article
Information
Parasitology , Volume 134 , Issue 10 , September 2007 , pp. 1315 - 1327
Copyright
Copyright © Cambridge University Press 2007

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