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Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

Published online by Cambridge University Press:  06 April 2009

D. C. De Graaf ,
P. Berghen ,
L. Moens ,
T. M. De Marez ,
S. Raes ,
M. L. Blaxter  and
J. Vercruysse
D. C. De Graaf
Affiliation:
Department of Parasitology, University of Gent, Salisburylaan 133, B-9820 Merelbeke, Belgium
P. Berghen
Affiliation:
Department of Parasitology, University of Gent, Salisburylaan 133, B-9820 Merelbeke, Belgium
L. Moens
Affiliation:
Department of Biochemistry, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium
T. M. De Marez
Affiliation:
Department of Parasitology, University of Gent, Salisburylaan 133, B-9820 Merelbeke, Belgium
S. Raes
Affiliation:
Department of Parasitology, University of Gent, Salisburylaan 133, B-9820 Merelbeke, Belgium
M. L. Blaxter
Affiliation:
Institute of Cell, Animal and Population Biology, Ashworth Laboratories, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, UK
J. Vercruysse*
Affiliation:
Department of Parasitology, University of Gent, Salisburylaan 133, B-9820 Merelbeke, Belgium
*
*Corresponding author: University of Gent, Faculty of Veterinary Medicine, Department of Parasitology, Salisburylaan 133, B-9820 Merelbeke, Belgium. Tel: + 32 9 264 7400. Fax: +32 9 264 74 96.
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Summary

Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle of O. ostertagi adults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected by O. ostertagi and/or Cooperia oncophora and the negative controls.

Keywords

nematodeOstertagia ostertagihaemoglobin
Type
Research Article
Information
Parasitology , Volume 113 , Issue 1 , July 1996 , pp. 63 - 69
Copyright
Copyright © Cambridge University Press 1996

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