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Schistosoma japonicum: some parameters affecting the development of protective immunity induced by a cryopreserved, irradiated schistosomula vaccine in guinea-pigs

Published online by Cambridge University Press:  06 April 2009

S. Xu ,
F. Shi  and
H. Wu
S. Xu
Affiliation:
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, No. 1, Lane 106, Shi-long Road, Shanghai 200030, People's Republic of, China
F. Shi
Affiliation:
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, No. 1, Lane 106, Shi-long Road, Shanghai 200030, People's Republic of, China
H. Wu
Affiliation:
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, No. 1, Lane 106, Shi-long Road, Shanghai 200030, People's Republic of, China
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Summary

Experiments were conducted in guinea-pigs to elucidate the parameters affecting the development of protective immunity against Schistosoma japonicum induced by a cryopreserved, irradiated schistosomula vaccine such as the number of immunizations, route of injection and the use of adjuvants. Results obtained indicated that the cryopreserved, irradiated schistosomula vaccine was effective by either intradermal or intramuscular injection. One intradermal injection with BCG adjuvant resulted in an average worm reduction of 50·24%, only a little lower than that of a non-cryopreserved, irradiated vaccine, 53·55%, with no statistically significant difference between the two. By intramuscular injection the worm reduction was lower (max. 40%) whether given with or without adjuvants or in 1 or 2 injections.

Keywords

Schistosoma japonicumguinea-pigscryopreservedirradiated vaccineprotective immunity parameters
Type
Research Article
Information
Parasitology , Volume 102 , Issue 1 , February 1991 , pp. 45 - 47
Copyright
Copyright © Cambridge University Press 1991

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References

Bickle, Q. D., Taylor, M. G., Doenhoff, M. J. & Wilson, G. S. (1979). Immunization of mice with gamma-irradiated intramuscularly injected schistosomula of Schistosoma mansoni. Parasitology 79, 209–22.Google Scholar
Eveland, L. K. & Morse, S. I. (1978). Schistosoma mansoni: infectivity and immunizing effects of in vitro derived schistosomula attenuated by X-irradiation. Experimental Parasitology 45, 19–25.CrossRefGoogle ScholarPubMed
Hsu, S. Y. L., Hsu, S. F. & Burmeister, L. F. (1981). Schistosoma mansoni: vaccination of mice with highly X-irradiated cercariae. Experimental Parasitology 52, 91–104.CrossRefGoogle ScholarPubMed
James, E. R. (1981). Schistosoma mansoni: cryopreservation of schistosomules by two-step addition of ethanediol and rapid cooling. Experimental Parasitology 52, 105–16.Google Scholar
Lewis, F. A., Stirewalt, M. A. & Leef, J. L. (1984). Schistosoma mansoni: vaccination of mice with 10-krad-irradiated cryopreserved schistosomules. Experimental Parasitology 57, 261–8.CrossRefGoogle ScholarPubMed
Minard, P., Dean, D. A., Jacobson, R. H., Vannier, W. E. & Murrell, K. D. (1978). Immunization of mice with Cobalt-60 irradiated Schistosoma mansoni cercariae. American Journal of Tropical Medicine and Hygiene 27, 76–86.CrossRefGoogle ScholarPubMed
Mountford, A. P., Coulson, P. S. & Wilson, R. A. (1988). Antigen localization and the induction of resistance in mice vaccinated with irradiated cercariae of Schistosoma mansoni. Parasitology 97, 11–25.CrossRefGoogle ScholarPubMed
Murrell, K. D., Stirewalt, M. A. & Lewis, F. A. (1979). Schistosoma mansoni: vaccination of mice with cryopreserved irradiated schistosomules. Experimental Parasitology 48, 265–71.Google Scholar
Pearce, C. J. & McLaren, D. J. (1983). Schistosoma mansoni: in vitro and in vivo studies of immunity using the guinea pig model. Parasitology 87, 465–79.CrossRefGoogle ScholarPubMed
Stirewalt, M. A., Lewis, F. A., Cousin, C. E. & Leef, J. L. (1984). Cryopreservation of schistosomules of Schistosoma mansoni in quantity. American Journal of Tropical Medicine and Hygiene 33, 116–24.CrossRefGoogle ScholarPubMed
Xu, S., Shi, F., Zhong, Z., Xu, C., Liu, X., Wu, H. & Fu, L. (1989 a). Experiments on the method of cryo-storage of schistosomula of S. japonicum. Chinese Journal of Veterinary Science and Technology 8, 20–2.Google Scholar
Xu, S., Wu, H. & Fu, L. (1989 b). Immunological experiments in mice with cryopreserved schistosomular vaccine of S. japonicum. Chinese Journal of Veterinary Science and Technology 12, 8–10.Google Scholar
Xu, S., Shi, F., Wu, H., Fu, L., Tzian, T., Lin, B., Chang, T. & Chu, H. (1990 a). Immunological experiments in sheep with cryopreserved schistosomular vaccine of S. japonicum. Chinese Journal of Veterinary Science and Technology 2, 19–20.Google Scholar
Xu, S., Shi, F., Fu, L. & Wu, H. (1990 b). Studies on the combined method – a modified method of cryopreserving schistosomula. Chinese Journal of Veterinary Science and Technology 6 (In press).Google Scholar