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Long-term observation of subcutaneous tissue reaction to synthetic auditory ossicle (Apaceram®) in rats

Published online by Cambridge University Press:  29 June 2007

Dong-Jun Li ,
Katsuichiro Ohsaki ,
Kunio Ii ,
Qing Ye ,
Yoko Nobuto ,
Satoru Tenshin  and
Teruko Takano-Yamamoto
Dong-Jun Li
Affiliation:
Division of Clinical Otology, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
Katsuichiro Ohsaki*
Affiliation:
Division of Clinical Otology, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
Kunio Ii
Affiliation:
1st Department of Pathology, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
Qing Ye
Affiliation:
1st Department of Pathology, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
Yoko Nobuto
Affiliation:
Division of Clinical Otology, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
Satoru Tenshin
Affiliation:
School of Medicine and the Department of Orthodontics, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
Teruko Takano-Yamamoto
Affiliation:
School of Medicine and the Department of Orthodontics, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
*
Address for correspondence: Katsuichiro Ohsaki, M.D., Ph.D., Division of Clinical Otology, University Hospital, School of Medicine, The University of Tokushima, Kuramoto 2-50-1, Tokushima 770, Japan. Fax: +81-886-33-7208
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Abstract

The present study evaluates histological characteristics of the soft tissue response to long-term implantation of Apaceram™ discs composed of dense hydroxyapatite in rats. Discs were implanted into the subcutaneous tissue of 76 rats for six to 20 months. Decalcified histological sections stained with haematoxylin and eosin (H & E) and Mallory's azan were examined. Different cell types surrounding implants were counted. The greatest proportion of macrophages was found at six months (13.5 per cent). This proportion gradually decreased to four per cent at 20 months. Small numbers of lymphocytes and foreign body giant cells were observed in every group, but neither neutrophils nor osteogenesis were observed in any specimens. Results of the present study and previous related studies indicate that despite reappearance of a small number of macrophages six months after implantation, Apaceram™ is useful for reconstructive surgery.

Keywords

HydroxyapatitesRats
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 8 , August 1997 , pp. 702 - 706
Copyright
Copyright © JLO (1984) Limited 1997

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Footnotes

This paper was presented 17–19 October 1996 at the conference of the Otological Society of Japan in Tokyo, organized by the Department of Otolaryngology, School of Medicine, Keio University, Tokyo 160, Japan.

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