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Studies on cryopreservation of third-stage larvae of Parastrongylus cantonensis

Published online by Cambridge University Press:  05 June 2009

David Chao ,
Lian-Chen Wang  and
Eng-Rin Chen
David Chao
Affiliation:
Department of Biology, National Sun Yat-sen University
Lian-Chen Wang
Affiliation:
Department of Biology, National Sun Yat-sen University Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China
Eng-Rin Chen
Affiliation:
Department of Parasitology, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China
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Abstract

Attempts have been made to increase the survival rate of third-stage larvae of Parastrongylus cantonensis during cryopreservation. Parasites were obtained from experimentally infected snails, Biomphalaria glabrata. The maximum non-toxic concentrations of cryoprotectants dimethyl sulphoxide (DMSO) and glycerol were determined as 10% v/v in NCTC 109 medium. Different pre-cooling times and serum additives were assayed to determine their effects on the viability of larvae. Larvae pre-cooled at –20°C for 15–20 minutes before transfer to –70°C had the optimum survival rate. The presence of serum was essential for further cooling to –196°C. There were no differences between native and heat-inactivated sera on the larval viability. The survival of larvae recovered after storage at –196°C was higher in sera collected from the rat, man, or fetal calf than in sera collected from the mouse, dog, or horse. Viability assessed by mouse-infections showed that thawed larvae retained infectivity for susceptible hosts, but it was significantly lower than that of unfrozen control worms.

Keywords

Parastrongylus cantonensiscryopreservationthird-stage larvaeNematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 1 , March 1993 , pp. 63 - 68
Copyright
Copyright © Cambridge University Press 1993

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