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Detection of antibodies to Anisakis simplex larvae by enzyme-linked immunosorbent assay and immunoelectrophoresis using crude or purified antigens

Published online by Cambridge University Press:  05 June 2009

R. Gutiérrez Ramos  and
M. Tsuji
R. Gutiérrez Ramos
Affiliation:
Department of Parasitology, School of Medicine, Hiroshima University, Minami Ku, Hiroshima 734, Japan
M. Tsuji
Affiliation:
Department of Parasitology, School of Medicine, Hiroshima University, Minami Ku, Hiroshima 734, Japan
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Abstract

The enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) were used for the serodiagnosis of larval Anisakis simplex infections in man and immunized rabbits. Sephacryl S-300 gel filtration was used for separating crude antigen. Four fractions were obtained. Sera from patients with other helminth infections sometimes cross reacted with Anisakis larval antigens. With IEP, crude antigen is more sensitive than purified antigens. With ELISA, the third fraction is the most sensitive for detecting antibodies to Anisakis larvae in the sera of humans and immunized rabbits.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 4 , December 1994 , pp. 305 - 309
Copyright
Copyright © Cambridge University Press 1994

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