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Effects of intraruminal infusions of urea, sucrose or urea plus sucrose on plasma urea and glucose kinetics in sheep fed chopped lucerne hay

Published online by Cambridge University Press:  27 March 2009

Y. Obara  and
D. W. Dellow
Y. Obara
Affiliation:
Biotechnology Division, Department of Scientific and Industrial Research, Private Bag, Palmerston North, New Zealand
D. W. Dellow
Affiliation:
Biotechnology Division, Department of Scientific and Industrial Research, Private Bag, Palmerston North, New Zealand
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Summary

The effect of rumen fermentation on the relationship between urea and glucose kinetics was examined in sheep fed chopped lucerne hay with intraruminal infusions of water, urea, sucrose, or urea plus sucrose at Palmerston North, New Zealand in 1986. Sheep were fed hourly and infused intraruminally with water (1200 m1/day), or a similar volume containing either urea alone (13·7g/day), sucrose alone (178·2 g/day) or urea (14·6 g/day) plus sucrose (175·0 g/day). The added sucrose resulted in a lower rumen ammonia concentration (P < 0·05), lower plasma urea concentration (P < 0·05) and reduced urinary urea excretion (P < 0·05). Urea recycled to the gut tended to increase with the sucrose, urea or sucrose plus urea treatments compared with the water treatment. The fermentation of sucrose in the rumen resulted in decreases in ruminal pH (P < 0·05) and in the ratio of acetate to propionate (A:P) (P < 0·05). The infusion of sucrose also increased the concentration of propionate in rumen fluid (P < 0·05), tended to increase the plasma glucose level and increased plasma glucose irreversible loss (P < 0·05). The infusion of urea resulted in an increase in the plasma urea level (P < 0·05), urea pool size (P < 0·05) and urea irreversible loss (P < 0·01). However, urea infusion did not affect glucose metabolism or volatile fatty acid (VFA) fermentation. The effects of sucrose infusion on glucose and urea kinetics were broadly similar when given alone or with urea, apart from changes in the urea degradation rate. It was concluded that the additional fermentative activity resulting from sucrose increased propionate production which, in turn, was available for glucose production, thus ‘sparing’ amino acids for tissue protein utilization and reducing urea excretion.

Type
Animals
Information
The Journal of Agricultural Science , Volume 121 , Issue 1 , August 1993 , pp. 125 - 130
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Barker, S. B. & Summerson, W. H. (1941). The colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry 138, 535–554.CrossRefGoogle Scholar
Black, A. L., Egan, A. R., Anand, R. S. & Chapman, T. E. (1968). The role of amino acids in gluconeogenesis in lactating ruminants. In Isotope Studies on the Nitrogen Chain, pp. 247–263. Vienna: Interational Atomic Energy Agency.Google Scholar
Bruckental, I., Oldham, J. D. & Sutton, J. D. (1980). Glucose and urea kinetics in cows in early lactation. British Journal of Nutrition 44, 33–45.CrossRefGoogle ScholarPubMed
Cocimano, M. R. & Leng, R. A. (1967). Metabolism of urea in sheep. British Journal of Nutrition 21, 353–371.CrossRefGoogle ScholarPubMed
Coulombe, J. J. & Favreav, L. (1963). A new simple semimicromethod for colorimetric determination of urea. Clinical Chemistry 9, 102–108.Google Scholar
Dellow, D. W., Obara, Y., Kelly, K. E. & Sinclair, B. R. (1988). Improving the efficiency of utilisation of pasture protein by sheep. Proceedings of the New Zealand Society of Animal Production 48, 253–255.Google Scholar
Herbein, J. H., Van Maanen, R. W., McGilliard, A. D. & Young, J. W. (1978). Rumen propionate and blood glucose kinetics in growing cattle fed isoenergetic diets. Journal of Nutrition 108, 994–1001.CrossRefGoogle ScholarPubMed
Houpt, T. R. (1959). Utilization of blood urea in ruminants. American Journal of Physiology 197, 115–120.CrossRefGoogle ScholarPubMed
Hunter, G. D. & Millson, G. C. (1964). Gluconeogenesis in the lactating dairy cow. Research in Veterinary Science 5, 1–6.CrossRefGoogle Scholar
Ishaque, M., Thomas, P. C. & Rook, J. A. F. (1971). Consequences to the host of changes in rumen microbial activity. Nature New Biology 231, 253–256.CrossRefGoogle Scholar
Janes, A. N., Weekes, T. E. C. & Armstrong, D. G. (1985). Absorption and metabolism of glucose by the mesentericdrained viscera of sheep fed on dried-grass or ground, maize-based diets. British Journal of Nutrition 54, 449–458.CrossRefGoogle ScholarPubMed
Kennedy, P. M. (1980). The effects of dietary sucrose and the concentrations of plasma urea and rumen ammonia on the degradation of urea in the gastrointestinal tract of cattle. British Journal of Nutrition 43, 125–140.CrossRefGoogle ScholarPubMed
Kennedy, P. M. & Milligan, L. P. (1980). The degradation and utilization of endogenous urea in the gastrointestinal tract of ruminants: a review. Canadian Journal of Animal Science 60, 205–221.Google Scholar
Kennedy, P. M., Clarke, R. T. J. & Milligan, L. P. (1981). Influences of dietary sucrose and urea on transfer of endogenous urea to the rumen of sheep and numbers of epithelial bacteria. British Journal of Nutrition 46, 533–541.CrossRefGoogle Scholar
König, B. A., Oldham, J. D. & Parker, D. S. (1984). The effect of abomasal infusion of casein on acetate, palmitate and glucose kinetics in cows during early lactation. British Journal of Nutrition 52, 319–328.CrossRefGoogle ScholarPubMed
McIntyre, K. H. (1971). The effects of continuous intravenous and intraruminal infusions of urea on nitrogen metabolism in sheep. Australian Journal of Agricultural Research 22, 429–441.CrossRefGoogle Scholar
Obara, Y. & Shimbayashi, K. (1988). Quantitative aspects of appearance of recycled urea in the digestive tract of goats. Japanese Agriculture Research Quarterly 21, 284–290.Google Scholar
Obara, Y., Dellow, D. W. & Nolan, J. V. (1991). The influence of energy-rich supplements on nitrogen kinetics in ruminants. In Physiological Aspects of Digestion and Metabolism in Ruminants (Eds Tsuda, T., Sasaki, Y. & Kawashima, R.), pp. 515–539. San Diego: Academic Press.CrossRefGoogle Scholar
Perston, T. R. & Leng, R. A. (1974). Supplementation of diets based on fibrous residues and by-products. In Straw and Other Fibrous By-products as Feed (Eds Sundstol, F. & Owen, E.), pp. 373–413. Amsterdam: Elsevier Science.Google Scholar
Schmidt, S. P., Smith, J. A. & Young, J. W. (1974). Rapid determination of [carbon- 14]glucose specific radioactivity for in vivo glucose kinetics. Journal of Dairy Science 58, 952–956.CrossRefGoogle Scholar
Snedecor, G. W. & Cochran, G. (1967). Statistical Methods, 6th edn.Ames: Iowa State University Press.Google Scholar
Somogyi, M. (1945). The determination of blood sugar. Journal of Biological Chemistry 160, 69–73.CrossRefGoogle Scholar
Spires, H. R. & Clark, J. H. (1979). Effect of intraruminal urea administration on glucose metabolism in dairy steers. Journal of Nutrition 109, 1438–1447.Google ScholarPubMed
Walker, D. J., Egan, A. R., Nader, C. J., Ulyatt, M. J. & Storer, G. B. (1975). Rumen microbial protein synthesis and proportions of microbial and non-microbial nitrogen flowing to the intestines of sheep. Australian Journal of Agricultural Research 26, 699–708.Google Scholar
Weatherburn, M. W. (1967). Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry 39, 971–974.CrossRefGoogle Scholar