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The localization of G6pd, glucose-6-phosphate dehydrogenase, and mdx, muscular dystrophy in the mouse X chromosome

Published online by Cambridge University Press:  14 April 2009

J. Peters ,
S. T. Ball ,
D. J. Charles ,
W. Pretsch ,
Grahame Bulfield ,
D. Miller  and
V. M. Chapman
J. Peters*
Affiliation:
MRC Radiobiology Unit, Chilton, Didcot, Oxon OXll ORD, UK
S. T. Ball
Affiliation:
MRC Radiobiology Unit, Chilton, Didcot, Oxon OXll ORD, UK
D. J. Charles
Affiliation:
Institut für Säugetiergenetik, Gesellschaft für Strahlen and Umweltforschung, 8042 Neuherberg, Federal Republic of Germany
W. Pretsch
Affiliation:
Institut für Säugetiergenetik, Gesellschaft für Strahlen and Umweltforschung, 8042 Neuherberg, Federal Republic of Germany
Grahame Bulfield
Affiliation:
Gene Expression Group, AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian EH25 9PS, UK
D. Miller
Affiliation:
Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, NY 14263 USA
V. M. Chapman
Affiliation:
Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, NY 14263 USA
*
* Corresponding author.
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A low activity mutant of glucose-6-phosphate dehydrogenase, G6pda-m1Neu has been used to position G6pd in the mouse X chromosome. Linkage tests with tabby, Ta and harlequin, Hq, indicate a likely gene order of Hq–G6pd–Ta. Muscular dystrophy, mdx, has been located by two-and three-point crosses using Hprt, Pgk-1 and Moblo and suggest a gene order of Hprt–mdx–Pgk-1–Moblo. Together with existing linkage data a tentative order for the seven loci is Hq–Hprt–G6pd–mdx–Ta–Pgk-1–Moblo. The relative positions of G6pd and mdx have not been directly tested and G6pd is assigned provisionally proximal to mdx. In the three point test using Hq, G6pd and Ta the recombination frequency found between Hq and Ta was 9·9 ± 2·6%, substantially less than the value of 20·5 ± 2·1% reported by Isaacson et al. (1974).

Type
Research Article
Information
Genetics Research , Volume 52 , Issue 3 , December 1988 , pp. 195 - 201
Copyright
Copyright © Cambridge University Press 1988

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