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Inheritance of Cry1F resistance, cross-resistance and frequency of resistant alleles in Spodoptera frugiperda (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  14 August 2013

A.M. Vélez ,
T.A. Spencer ,
A.P. Alves ,
D. Moellenbeck ,
R.L. Meagher ,
H. Chirakkal  and
B.D. Siegfried
A.M. Vélez
Affiliation:
Department of Entomology, University of Nebraska, Lincoln, Nebraska 68583, US
T.A. Spencer
Affiliation:
Department of Entomology, University of Nebraska, Lincoln, Nebraska 68583, US
A.P. Alves
Affiliation:
Dupont Pioneer, Johnston, Iowa, USA
D. Moellenbeck
Affiliation:
DM Crop Research Group Inc., Polk City, Iowa, US
R.L. Meagher
Affiliation:
USDA-ARS CMAVE, Gainesville, Florida, USA
H. Chirakkal
Affiliation:
Department of Biological Sciences, University of Nebraska, Lincoln, Nebraska, US
B.D. Siegfried*
Affiliation:
Department of Entomology, University of Nebraska, Lincoln, Nebraska 68583, US
*
*Author for correspondence Fax: +1 (402) 472-4687 Phone: +1 (402) 472-8714 E-mail: bsiegfried1@unl.edu
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Abstract

Transgenic maize, Zea maize L., expressing the Cry1F protein from Bacillus thuringiensis has been registered for Spodoptera frugiperda (J. E. Smith) control since 2003. Unexpected damage to Cry1F maize was reported in 2006 in Puerto Rico and Cry1F resistance in S. frugiperda was documented. The inheritance of Cry1F resistance was characterized in a S. frugiperda resistant strain originating from Puerto Rico, which displayed >289-fold resistance to purified Cry1F. Concentration–response bioassays of reciprocal crosses of resistant and susceptible parental populations indicated that resistance is recessive and autosomal. Bioassays of the backcross of the F1 generation crossed with the resistant parental strain suggest that a single locus is responsible for resistance. In addition, cross-resistance to Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ba, Cry2Aa and Vip3Aa was assessed in the Cry1F-resistant strain. There was no significant cross-resistance to Cry1Aa, Cry1Ba and Cry2Aa, although only limited effects were observed in the susceptible strain. Vip3Aa was highly effective against susceptible and resistant insects indicating no cross-resistance with Cry1F. In contrast, low levels of cross-resistance were observed for both Cry1Ab and Cry1Ac. Because the resistance is recessive and conferred by a single locus, an F1 screening assay was used to measure the frequency of Cry1F-resistant alleles from populations of Florida and Texas in 2010 and 2011. A total frequency of resistant alleles of 0.13 and 0.02 was found for Florida and Texas populations, respectively, indicating resistant alleles could be found in US populations, although there have been no reports of reduced efficacy of Cry1F-expressing plants.

Keywords

fall armywormBt maizeCry1Finheritanceresistancecross-resistanceF1 screenresistance managementhigh-dose refuge strategy
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 103 , Issue 6 , December 2013 , pp. 700 - 713
Copyright
Copyright © Cambridge University Press 2013 

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