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Plant Genetic Resources (2007), 5: 154-171 Cambridge University Press
doi:10.1017/S1479262107835659
Published online by Cambridge University Press 22 Nov 2007
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Plant Genetic Resources: Characterization and Utilization (2007), 5:154-171 Cambridge University Press
Copyright © NIAB 2007
doi:10.1017/S1479262107835659

Research Article

Genetic diversity within Lablab purpureus and the application of gene-specific markers from a range of legume species

S. C. Venkateshaa1, M. Byre Gowdaa1, P. Mahadevua1, A. Mohan Raoa1, D.-J. Kima2, T. H. N. Ellisa3 and M. R. Knoxa3 c1

a1 University of Agricultural Sciences, GKVK Campus, Bangalore 560065 Karnataka, India
a2 International Institute of Tropical Agriculture (IITA), C/O Biosciences Eastern and Central Africa (BECA). PO Box 30709, Nairobi, Kenya
a3 John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK
Article author query
venkatesha sc PubMed  Google Scholar
byre gowda m PubMed  Google Scholar
mahadevu p PubMed  Google Scholar
mohan rao a PubMed  Google Scholar
kim d- PubMed  Google Scholar
ellis th PubMed  Google Scholar
knox mr PubMed  Google Scholar

Abstract

Molecular markers have been used to study genetic diversity within a set of Lablab purpureus accessions collected from the southern states of India. Amplified fragment length polymorphism (AFLP) molecular marker studies using a total of 78 L. purpureus accessions with nine primer combinations showed there was very little genetic diversity within the L. purpureus accessions from the southern Indian germplasm collection as compared to a set of 15 accessions from other international germplasm collections that included African accessions. The set of 15 were selected from a random amplified length polymorphism (RAPD) marker study and chosen on the basis of widest genetic distance. Further molecular analysis with polymerase chain reaction (PCR) markers from 97 expressed sequence tag (EST) and gene-specific primer pairs, designed from a range of legume sequences, concurred with the AFLP analyses. Both of these approaches provide a wealth of markers for diversity and mapping studies. The 97 sequence-specific primer pairs tested in L. purpureus resulted in 70% amplification success, with 44% of primer pairs amplifying single bands and 10% double bands. Markers generated from these EST and genomic sequences provide useful cross-reference to comparative legume genomics that will potentially have long-term benefit to legume plant breeding.

(Received August 15 2006)

(Accepted October 20 2006)

Key Words: AFLP; gene-specific markers; genetic diversity; Lablab purpureus; legumes; Medicago truncatula

Correspondence:

c1 Corresponding author. knox@bbsrc.ac.uk

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