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Supplementation of sperm media with zinc, D-aspartate and co-enzyme Q10 protects bull sperm against exogenous oxidative stress and improves their ability to support embryo development

Published online by Cambridge University Press:  07 March 2017

Vincenza Barbato ,
Riccardo Talevi ,
Sabrina Braun ,
Anna Merolla ,
Sam Sudhakaran ,
S. Longobardi  and
Roberto Gualtieri
Vincenza Barbato
Affiliation:
Dipartimento di Biologia, Università di Napoli ‘Federico II’, Complesso Universitario di Monte S Angelo, Via Cinthia, 80126 Napoli, Italy.
Riccardo Talevi
Affiliation:
Dipartimento di Biologia, Università di Napoli ‘Federico II’, Complesso Universitario di Monte S Angelo, Via Cinthia, 80126 Napoli, Italy.
Sabrina Braun
Affiliation:
Dipartimento di Biologia, Università di Napoli ‘Federico II’, Complesso Universitario di Monte S Angelo, Via Cinthia, 80126 Napoli, Italy.
Anna Merolla
Affiliation:
Dipartimento di Biologia, Università di Napoli ‘Federico II’, Complesso Universitario di Monte S Angelo, Via Cinthia, 80126 Napoli, Italy.
Sam Sudhakaran
Affiliation:
Dipartimento di Biologia, Università di Napoli ‘Federico II’, Complesso Universitario di Monte S Angelo, Via Cinthia, 80126 Napoli, Italy.
S. Longobardi
Affiliation:
Medical Affairs Fertility, Merck KGaA, Frankfurter Straße 250, 64293 Darmstadt, Germany.
Roberto Gualtieri*
Affiliation:
Dipartimento di Biologia, Università di Napoli ‘Federico II’, Complesso Universitario di Monte S Angelo, Via Cinthia, 80126 Napoli, Italy.
*
All correspondence to: R. Gualtieri. Dipartimento di Biologia, Università di Napoli ‘Federico II’, Complesso Universitario di Monte S Angelo, Via Cinthia, 80126 Napoli, Italy. Tel: +39 081 679212. Fax: +39 081/679233. E-mail: roberto.gualtieri@unina.it
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Summary

High levels of reactive oxygen species in the semen of infertile patients or spontaneously generated during in vitro sperm handling may impair sperm quality, fertilization and embryo developmental competence. We recently reported that zinc, d-aspartate and co-enzyme Q10, contained in the dietary supplement Genadis® (Merck Serono), have protective effects on human and bull sperm motility, lipid peroxidation and DNA fragmentation in vitro; furthermore, in bovine, treated spermatozoa had an improved ability to support embryo development. However, only a few studies have investigated the protective role of antioxidants during in vitro sperm handling in the presence of an exogenous oxidative stress. Herein, to simulate such conditions in an animal model, we induced exogenous oxidative stress on spermatozoa through the xanthine–xanthine oxidase system and investigated its effects on sperm function and subsequent embryo developmental competence in the presence of zinc, d-Asp and CoQ10 protection. The main results showed that exogenous oxidative stress decreased sperm motility, increased sperm DNA fragmentation, and reduced fertilization and blastocyst rates and quality. Pre-treatment with zinc, d-aspartate and co-enzyme Q10 before exogenous oxidative stress was able to prevent these effects. Supplementation of sperm culture media with zinc, d-aspartate and co-enzyme Q10 could protect sperm from oxidative stress damage during in vitro handling in assisted reproductive technologies.

Keywords

AntioxidantsDNA fragmentationEmbryo developmentOxidative stressSpermatozoa
Type
Research Article
Information
Zygote , Volume 25 , Issue 2 , April 2017 , pp. 168 - 175
Copyright
Copyright © Cambridge University Press 2017 

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