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Role of arachidonic acid cascade in Rhinella arenarum oocyte maturation

Published online by Cambridge University Press:  25 June 2014

Maria Eugenia Ortiz ,
Ana Josefina Arias-Torres  and
Liliana Isabel Zelarayán
Maria Eugenia Ortiz
Affiliation:
Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, Tucumán, Argentina.
Ana Josefina Arias-Torres
Affiliation:
Instituto de Biología, Facultad de Bioqca., Qca. y Farmacia, UNT. INSIBIO-CONICET, Chacabuco 461, Tucumán, Argentina.
Liliana Isabel Zelarayán*
Affiliation:
Instituto de Biología, Facultad de Bioqca., Qca. y Farmacia, UNT. INSIBIO-CONICET, Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina.
*
All correspondence to: Liliana Isabel Zelarayán. Instituto de Biología, Facultad de Bioqca., Qca. y Farmacia, UNT. INSIBIO-CONICET, Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina. Tel: +549 381 4247752 x7093. Fax: +549 381 4247752 x7004. e-mail: lzelarayan@fbqf.unt.edu.ar
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Summary

There are no studies that document the production of prostaglandins (PGs) or their role in Rhinella arenarum oocyte maturation. In this study, we analysed the effect of arachidonic acid (AA) and prostaglandins (PGs) on maturation, activation and pronuclear formation in R. arenarum oocytes. Our results demonstrated that AA was capable of inducing maturation in time-dependent and dose-dependent manner. Arachidonic acid-induced maturation was inhibited by indomethacin. PGs from AA hydrolysis, such as prostaglandin F2α (PGF2α) and, to a lesser extent, PGE2, induced meiosis resumption. Oocyte maturation in response to PGF2α was similar to that produced by progesterone (P4). Oocyte response to PGE1 was scarce. Rhinella arenarum oocyte PGF2α-induced maturation showed seasonal variation. From February to June, oocytes presented low sensitivity to PGF2α. In following periods, this response increased until a maximum was reached during October to January, a close temporal correlation with oocyte response to P4 being observed. The effect of PGF2α on maturation was verified by analysing the capacity of oocytes to activate and form pronuclei after being injected with homologous sperm. The cytological analysis of activated oocytes demonstrated the absence of cortical granules in oocytes, suggesting that PGF2α induces germinal vesicle breakdown (GVBD) and meiosis resumption up to metaphase II. In turn, oocytes matured by the action of PGF2α were able to form pronuclei after fertilization in a similar way to oocyte maturated by P4. In microinjection of mature cytoplasm experiments, the transformation of pre-maturation promoting factor (pre-MPF) to MPF was observed when oocytes were treated with PGF2α. In summary, our results illustrated the participation of the AA cascade and its metabolites in maturation, activation and pronuclei formation in R. arenarum.

Keywords

Arachidonic acid cascadeOocyte maturationProstaglandinsRhinella arenarum
Type
Research Article
Information
Zygote , Volume 23 , Issue 4 , August 2015 , pp. 603 - 614
Copyright
Copyright © Cambridge University Press 2014 

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