Hostname: page-component-8448b6f56d-wq2xx Total loading time: 0 Render date: 2024-04-19T04:44:44.436Z Has data issue: false hasContentIssue false
  • English
  • Français

Developmental potential of in vitro or in vivo matured oocytes

Published online by Cambridge University Press:  05 June 2013

Diego D. Alcoba ,
Anita M. Pimentel ,
Ilma S. Brum  and
Helena E. Corleta
Diego D. Alcoba*
Affiliation:
Laboratório de Biologia Molecular Endócrina e Tumoral, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, Instituto de Ciências Básicas da Saúde, 500 Porto Alegre, Rio Grande do Sul, Brazil.
Anita M. Pimentel
Affiliation:
Associação Hospitalar Moinhos de Vento, Núcleo de Reprodução Humana Gerar, Rua Ramiro Barcelos, 910–8° andar, Cj. 905, Porto Alegre, Rio Grande do Sul, Brazil.
Ilma S. Brum
Affiliation:
Laboratório de Biologia Molecular Endócrina e Tumoral, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, Instituto de Ciências Básicas da Saúde, 500, Porto Alegre, Rio Grande do Sul, Brazil.
Helena E. Corleta
Affiliation:
Laboratório de Ginecologia e Obstetrícia Molecular–Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos, 2350, Bairro Santa Cecília, Porto Alegre, Rio Grande do Sul, Brazil.
*
All correspondence to: Diego Duarte Alcoba. Laboratório de Biologia Molecular Endócrina e Tumoral, Universidade Federal do Rio Grande do Sul (UFRGS), Rua Sarmento Leite, Instituto de Ciências Básicas da Saúde, 500 Porto Alegre, Rio Grande do Sul, Brazil. Tel: +55 51 33083453. Fax: +55 51 33083656. e-mail: alcobadiego@hotmail.com
Get access Rights & Permissions [Opens in a new window]

Summary

This study compared the embryological features of mature and immature oocytes (different stages) collected from stimulated cycles of in vitro fertilization (IVF). Immature oocytes were identified, classified as PI (prophase I – germinal vesicle, GV) or MI (metaphase I), were matured in vitro and fertilized using the intra-cytoplasmic sperm injection (ICSI) technique. Fertilization potential, cleavage, and subsequent transfer/cryopreservation of the embryos derived from these in vitro matured oocytes were compared with those of in vivo matured oocytes (collected at the MII stage). The characteristics of embryos derived from gametes recovered in the MI and MII stages were similar. The fertilization rate of immature oocytes recovered in PI was significantly lower than that of MII oocytes (P = 0.031), and the cleavage rate of the PI group was also lower than that of the MI (P = 0.004) and MII (P < 0.001) groups. In vitro maturation of MI oocytes is a suitable alternative when immature oocytes are recovered, as their characteristics and development are similar to those of in vivo matured oocytes. Optimization of outcomes for PI oocytes will require development of techniques that can distinguish which of these gametes will mature and fertilize.

Keywords

Embryo developmentImmature stageIn vitro maturationStimulated cycle
Type
Research Article
Information
Zygote , Volume 23 , Issue 1 , February 2015 , pp. 93 - 98
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adona, P., Pires, P., Quetglas, M., Schwarz, K. & Leal, C. (2008). Prematuration of bovine oocytes with butyrolactone I: Effects on meiosis progression, cytoskeleton, organelle distribution and embryo development. Anim. Reprod. Sci. 108, 49–65.Google Scholar
Barnes, F.L., Kausche, A., Tiglias, J., Wood, C., Wilton, L. & Trounson, A. (1996). Production of embryos from in vitro-matured primary human oocytes. Fertil. Steril. 65, 1151–6.Google Scholar
Ben-Ami, I., Komsky, A., Bern, O., Kasterstein, E., Komarovsky, D. & Ron-El, R. (2011). In vitro maturation of human germinal vesicle-stage oocytes: role of epidermal growth factor-like growth factors in the culture medium. Hum. Reprod. 26, 76–81Google Scholar
Bodri, D., Vernaeve, V., Guillén, J.J., Vidal, R., Figueras, F. & Coll, O. (2006). Comparison between a GnRH antagonist and a GnRH agonist flare-up protocol in oocyte donors: a randomized clinical trial. Hum. Reprod. 21, 2246–51.Google Scholar
Cha, K.Y. & Chian, R.C. (1998). Maturation in vitro of immature human oocytes for clinical use. Hum. Reprod. Update 4, 103–20.CrossRefGoogle ScholarPubMed
De Vos, A., Van de Velde, H., Joris, H. & Van Steirteghem, A. (1999). In-vitro matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection. Hum. Reprod. 14, 1859–63.CrossRefGoogle ScholarPubMed
Dekel, N. (1995). Molecular control of meiosis. Trends Endocrinol. Metab. 6, 165–9.Google Scholar
Edirisinghe, W., Junk, S., Matson, L. & Yovich, J. (1997). Birth from cryopreserved embryos following in-vitro maturation of oocytes and intracytoplasmic sperm injection. Hum. Reprod. 12, 1056–8.Google Scholar
Edwards, R.G. (1965). Maturation in vitro of human ovarian oocytes. Lancet 2, 926–9.CrossRefGoogle ScholarPubMed
Huang, J.Y., Chian, R.C., Gilbert, L., Fleiszer, D., Holzer, H., Dermitas, E., Elizur, S.E., Gidoni, Y., Levin, D., Son, W.Y. & Tan, S.L. (2010). Retrieval of immature oocytes from unstimulated ovaries followed by in vitro maturation and vitrification: A novel strategy of fertility preservation for breast cancer patients. Am. J. Surg. 200, 177–83.Google Scholar
Jaroudi, K., Hollanders, J., Sieck, U., Roca, G., El Nour, A. & Coskun, S. (1997). Pregnancy after transfer of embryos which were generated from in-vitro matured oocytes. Hum. Reprod. 12, 857–9.Google Scholar
Kim, B.K., Lee, S.C., Kim, K.J., Han, C.H. & Kim, J.H. (2000). In vitro maturation, fertilization, and development of human germinal vesicle oocytes collected from stimulated cycles. Fertil. Steril. 74, 1153–8.Google Scholar
Le Du, A., Kadoch, I., Bourcigaux, N., Doumerc, S., Bourrier, M., Chevalier, N., Fanchin, R., Chian, R., Tachdjian, G., Frydman, R. & Frydman, N. (2005). In vitro oocyte maturation for the treatment of infertility associated with polycystic ovarian syndrome: the French experience. Hum. Reprod. 20, 420–4.Google Scholar
Lee, J.E., Kim, S.D., Jee, B.C., Suh, C.S. & Kim, S.H. (2011). Oocyte maturity in repeated ovarian stimulation. Clin. Exp. Reprod. Med. 38, 234–7.Google Scholar
Liu, J., Katz, E., Garcia, J., Compton, G. & Baramki, T. (1997). Successful in vitro maturation of human oocytes not exposed to human chorionic gonadotropin during ovulation induction, resulting in pregnancy. Fertil. Steril. 67, 566–8.CrossRefGoogle Scholar
Liu, J., Lu, G., Qian, Y., Mao, Y. & Ding, W. (2003). Pregnancies and births achieved from in vitro matured oocytes retrieved from poor responders undergoing stimulation in in vitro fertilization cycles. Fertil. Steril. 80, 447–9.Google Scholar
McAvey, B., Zapantis, A., Jindal, S.K., Lieman, H.J. & Polotsky, A.J. (2011). How many eggs are needed to produce an assisted reproductive technology baby: is more always better? Fertil. Steril. 96, 332–5.Google Scholar
Nagy, Z.P., Cecile, J., Liu, J., Loccufier, A., Devroey, P. & Van Steirteghem, A. (1996). Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report. Fertil. Steril. 65, 1047–50.Google Scholar
Oktay, K., Demirtas, E., Son, W.Y., Lostritto, K., Chian, R.C. & Tan, S.L. (2008). In vitro maturation of germinal vesicle oocytes recovered after premature luteinizing hormone surge: description of a novel approach to fertility preservation. Fertil. Steril. 89, 228.e219–22.Google Scholar
Russell, J.B. (1999). Immature oocyte retrieval with in-vitro oocyte maturation. Curr. Opin. Obstet. Gynecol. 11, 289–96.Google Scholar
Sathananthan, A., Selvaraj, K. & Trounson, A. (2000). Fine structure of human oogonia in the foetal ovary. Mol. Cell. Endocrinol. 161, 3–8.Google Scholar
Smith, S.D., Mikkelsen, A. & Lindenberg, S. (2000). Development of human oocytes matured in vitro for 28 or 36 hours. Fertil. Steril. 73, 541–4.Google Scholar
Son, W.Y., Lee, S.Y. & Lim, J.H. (2005). Fertilization, cleavage and blastocyst development according to the maturation timing of oocytes in in vitro maturation cycles. Hum. Reprod. 20, 3204–7.Google Scholar
Son, W.Y., Chung, J.T., Demirtas, E., Holzer, H., Sylvestre, C., Buckett, W., Chian, R.C. & Tan, S.L. (2008). Comparison of in-vitro maturation cycles with and without in-vivo matured oocytes retrieved. Reprod. Biomed. Online 17, 59–67.Google Scholar
Suikkari, A.M. & Söderström-Anttila, V. (2007). In-vitro maturation of eggs: is it really useful? Best Pract. Res. Clin. Obstet. Gynaecol. 21, 145–55.Google Scholar
Söderström-Anttila, V., Salokorpi, T., Pihlaja, M., Serenius-Sirve, S. & Suikkari, A.M. (2006). Obstetric and perinatal outcome and preliminary results of development of children born after in vitro maturation of oocytes. Hum. Reprod. 21, 1508–13.Google Scholar
Trounson, A., Wood, C. & Kausche, A. (1994). In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil. Steril. 62, 353–62.Google Scholar
Trounson, A., Anderiesz, C. & Jones, G. (2001). Maturation of human oocytes in vitro and their developmental competence. Reproduction 121, 51–75.Google Scholar
Tucker, M., Wright, G., Morton, P. & Massey, J. (1998). Birth after cryopreservation of immature oocytes with subsequent in vitro maturation. Fertil. Steril. 70, 578–9.Google Scholar