Hostname: page-component-8448b6f56d-dnltx Total loading time: 0 Render date: 2024-04-23T17:57:42.841Z Has data issue: false hasContentIssue false
  • English
  • Français

Xenia in bahiagrass: gene expression at initial seed formation

Published online by Cambridge University Press:  21 December 2018

Florencia I. Pozzi ,
Guillermo R. Pratta ,
Carlos A. Acuña  and
Silvina A. Felitti
Florencia I. Pozzi
Affiliation:
Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR), CONICET, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Parque Villarino, S2125ZAA Zavalla, Provincia de Santa Fe, Argentina
Guillermo R. Pratta
Affiliation:
Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR), CONICET, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Parque Villarino, S2125ZAA Zavalla, Provincia de Santa Fe, Argentina
Carlos A. Acuña
Affiliation:
Instituto de Botánica del Nordeste (IBONE), CONICET, Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Sargento Cabral 2131, W3402BKG Corrientes, Argentina
Silvina A. Felitti*
Affiliation:
Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR), CONICET, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Parque Villarino, S2125ZAA Zavalla, Provincia de Santa Fe, Argentina
*
Author for correspondence: Silvina A. Felitti, Email: felitti@iicar-conicet.gob.ar
Get access Rights & Permissions [Opens in a new window]

Abstract

Xenia is the direct effect of pollen genotype on the development and characteristics of the seed and fruit in the period that spans from fertilization to seed germination. Xenia effects cause phenotypic variations in the seed and fruit, which have importance for seed and fruit production but are not heritable to the progeny. Two hypotheses have been proposed as a mechanism for xenia: the hormonal hypothesis and the mobile mRNAs hypothesis. Although xenia effects have been studied in seeds and fruits in many crops, its effects and mechanism have been poorly studied at the molecular level. The aim of this work was to perform an initial screening of the xenia effect in the hybrid endosperm at the molecular level by differential gene expression among different pollen genotype sources from Paspalum notatum Flüggé. In order to characterize xenia effects and mechanisms, crosses were made between an emasculated mother plant with donors from two different pollen genotypes. RNA was isolated from ovaries 3 h after pollination. Some of the 24 differentially expressed transcript-derived fragments (DETDFs) provided relevant information. Four of those DETDFs were related to germination, pollen tube growth and pollen–pistil interaction. Seven DETDFs were associated with seed development and production. Finally, four DETDFs were predicted to encode for mobile mRNAs. These DETDFs might be involved in xenia effects and mechanisms in P. notatum.

Keywords

endospermgene expressionmobile mRNAspseudogamyseedxenia
Type
Research Paper
Information
Seed Science Research , Volume 29 , Issue 1 , March 2019 , pp. 29 - 37
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aarts, MG, Keijzer, CJ, Stiekema, WJ and Pereira, A (1995) Molecular characterization of the CER1 gene of Arabidopsis involved in epicuticular wax biosynthesis and pollen fertility. Plant Cell 7, 2115–2127.Google Scholar
Benstein, RM, Ludewig, K, Wulfert, S, Wittek, S, Gigolashvili, T, Frerigmann, H, Gierth, M, Flügge, U and Krueger, S (2013) Arabidopsis phosphoglycerate dehydrogenase1 of the phosphoserine pathway is essential for development and required for ammonium assimilation and tryptophan biosynthesis. Plant Cell 25, 5011–5029.Google Scholar
Boavida, LC, Shuai, B, Yu, H, Pagnussat, GC, Sundaresan, V and McCormick, S (2009) A collection of ds insertional mutants associated with defects in male gametophyte development and function in Arabidopsis thaliana. Genetics 181, 1369–1385.Google Scholar
Bourdenx, B, Bernard, A, Domergue, F, Pascal, S, Léger, A, Roby, D, Pervent, M, Vile, D, Haslam, RP, Napier, JA, Lessire, R and Joubès, J (2011) Overexpression of Arabidopsis ECERIFERUM1 promotes wax very-long-chain alkane biosynthesis and influences plant response to biotic and abiotic stresses. Plant Physiology 156, 29–45.Google Scholar
Brown, RC and Lemmon, BE (2007) The developmental biology of cereal endosperm. Plant Cell Monographs 8, 1–20.Google Scholar
Bullant, C and Gallais, A (1998) Xenia effects in maize with normal endosperm: l. Importance and stability. Crop Science 38, 1517–1525.Google Scholar
Burson, BL (1987) Pollen germination, pollen tube growth and fertilization following self and interspecific pollination of Paspalum species. Euphytica 36, 641–650.Google Scholar
Burton, GW (1948) The method of reproduction in common Bahia grass, Paspalum notatum. Journal of the American Society of Agronomy 40, 443–452.Google Scholar
Dag, A and Mizrahi, Y (2005) Effect of pollination method on fruit set and fruit characteristics in the vine cactus Selenicereus megalanthus (‘yellow pitaya’). Journal of Horticultural Sciences and Biotechnology 80, 618–622.Google Scholar
Darwin, C (1868) The Variation of Animals and Plants under Domestication. London, John Murray.Google Scholar
Denney, JO (1992) Xenia includes metaxenia. HortScience 27, 722–728.Google Scholar
Depetris, MB, Acuña, CA, Pozzi, FI, Quarin, CL and Felitti, SA (2018) Identification of genes related to endosperm balance number insensitivity in Paspalum notatum. Crop Science. doi: 10.2135/cropsci2017.04.0260Google Scholar
Dogterom, MH, Winston, ML and Mukai, A (2000) Effect of pollen load size and source (self, outcross) on seed and fruit production in highbush blueberry cv. ‘Bluecrop’ (Vaccinium corymbosum; Ericaceae). American Journal of Botany 87, 1584–1591.Google Scholar
Felitti, SA, Acuña, CA, Ortiz, JPA and Quarin, CL (2015) Transcriptome analysis of seed development in apomictic Paspalum notatum. Annals of Applied Biology. doi: 10.1111/aab.12206Google Scholar
Hajduch, M, Hearne, LB, Miernyk, JA, Casteel, JE, Joshi, T, Agrawal, GK, Song, Z, Zhou, M, Xu, D and Thelen, JJ (2010) Systems analysis of seed filling in Arabidopsis: using general linear modeling to assess concordance of transcript and protein expression. Plant Physiology 152, 2078–2087.Google Scholar
Kaczmarek, J, Bujak, H, Kadłubiec, W, Grochowski, L and Galek, R (2001) Analysis of xenia in F1 seeds of yellow-grain winter-rye lines. Plant Breeding and Seed Science 45, 4–10.Google Scholar
Kim, M, Canio, W, Kessler, S and Sinha, N (2001) Developmental changes due to long-distance movement of a homeobox fusion transcript in tomato. Science 293, 287–289.Google Scholar
Kudo, H and Harada, T (2007) A graft-transmissible RNA from tomato rootstock changes leaf morphology of potato scion. HortScience 42, 225–226.Google Scholar
Lalanne, E, Honys, D, Johnson, A, Borner, G, Lilley, K, Dupree, P, Grossniklaus, U and Twell, D (2004) SETH1 and SETH2, two components of the glycosylphosphatidylinositol anchor biosynthetic pathway, are required for pollen germination and tube growth in Arabidopsis. Plant Cell 16, 229–240.Google Scholar
Liu, Y (2008) A novel mechanism for xenia? HortScience 43, 706.Google Scholar
Liu, Y (2018) Darwin's pangenesis and certain anomalous phenomena. Advances in Genetics. https://doi.org/10.1016/bs.adgen.2018.05.009Google Scholar
Mansfield, SG and Briarty, LG (1991) Early embryogenesis in Arabidopsis thaliana. II. The developing embryo. Canadian Journal of Botany 69, 461–476.Google Scholar
Martínez, EJ, Esteban Hopp, H, Stein, J, Ortiz, JPA and Quarin, CL (2003) Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers. Molecular Breeding 12, 319–327.Google Scholar
Missbach, S, Weis, BL, Martin, R, Simm, S and Bohnsack, MT (2013) 40S ribosome biogenesis co-factors are essential for gametophyte and embryo development. PLoS ONE. doi: 10.1371/journal.pone.0054084Google Scholar
Pfaffl, NW, Tichopad, A, Prgomet, C and Neuvians, T (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: Best-Keeper – Excel-based tool using pair-wise correlations. Biotechnology Letters 26, 509–515.Google Scholar
Piotto, FA, Batagin-Piotto, KD, de Almeida, M and Oliveira, GCX (2013) Interspecific xenia and metaxenia in seeds and fruits of tomato. Scientia Agricola 70, 102–107.Google Scholar
Preuss, D, Lemieux, B, Yen, G and Davis, RW (1993) A conditional sterile mutation eliminates surface components from Arabidopsis pollen and disrupts cell signaling during fertilization. Genes and Development 7, 974–985.Google Scholar
Pozzobon, MT and Valls, JFM (1997) Chromosome number in germoplasma accessions of Paspalum notatum (Gramineae). Brazilian Journal of Genetics 20, 29–34.Google Scholar
Quarin, CL (1999) Effect of pollen source and pollen ploidy on endosperm formation and seed set in pseudogamous apomictic Paspalum notatum. Sexual Plant Reproduction 11, 331–335.Google Scholar
Rieger, R, Michaelis, A and Green, MM (1968) A Glossary of Genetics and Cytogenetics: Classical and Molecular, 3rd edn. Berlin Heidelberg, Springer-Verlag.Google Scholar
Rueden, CT, Schindelin, J, Hiner, MC, DeZonia, BE, Walter, AE, Arena, ET and Eliceiri, KW (2017). ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics 18, 529.Google Scholar
Sabelli, PA and Larkins, BA (2009) The development of endosperm in grasses. Plant Physiology 149, 14–26.Google Scholar
Salas-Muñoz, S, Rodríguez-Hernández, AA, Ortega-Amaro, MA, Salazar-Badillo, FB and Jiménez-Bremont, JF (2016) Arabidopsis AtDjA3 null mutant shows increased sensitivity to abscisic acid, salt, and osmotic stress in germination and post-germination stages. Frontiers in Plant Science. doi: 10.3389/fpls.2016.00220Google Scholar
Sambrook, J and Russell, DW (2001) Molecular Cloning: A Laboratory Manual. New York, Cold Spring Harbor Laboratory Press.Google Scholar
Simon, P (2003) Q-Gene: processing quantitative real-time RT-PCR data. Bioinformatics 19, 1439–1440.Google Scholar
Stölting, KN, Gort, G, Wüst, C and Wilson, AB (2009) Eukaryotic transcriptomics in silico: optimizing cDNA-AFLP efficiency. BMC Genomics 10, 565–579.Google Scholar
Swingle, WT (1928) Metaxenia in the date palm – possibly a hormone action by the embryo or endosperm. Journal of Heredity 19, 257–268.Google Scholar
Thieme, C, Rojas-Triana, M, Stecyk, E, Schudoma, C, Zhang, W, Yang, L, Miñambres, M, Walther, D, Schulze, W and Paz-Ares, J (2015) Endogenous Arabidopsis messenger RNAs transported to distant tissues. Nature Plants 1, e15025.Google Scholar
Tischler, CR and Burson, BL (1995) Evaluating different bahiagrass cytotypes for heat tolerance and leaf epicuticular wax content. Euphytica 84, 229–235.Google Scholar
Toujani, W, Muñoz-Bertomeu, J, Flores-Tornero, M, Rosa-Téllez, S, Djoro Anoman, A and Ros, R (2013) Identification of the phosphoglycerate dehydrogenase isoform EDA9 as the essential gene for embryo and male gametophyte development in Arabidopsis. Plant Signaling & behavior 8, 11; e27207, doi: 10.4161/psb.27207Google Scholar
Voiniciuc, C, Günl, M, Schmidt, MH and Usadel, B (2015) Highly branched xylan made by IRREGULAR XYLEM14 and MUCILAGE-RELATED21 links mucilage to Arabidopsis seeds. Plant Physiology 169, 2481–2495.Google Scholar
Vuylsteke, M, Peleman, JD and van Eijk, MJT (2007) AFLP based transcript profiling (cDNA-AFLP) for genome-wide expression analysis. Nature Protocols 2, 1399–1413.Google Scholar
Wang, H, Boavida, LC, Ron, M and McCormick, S (2008a) Truncation of a protein disulfide isomerase, PDIL2-1, delays embryo sac maturation and disrupts pollen tube guidance in Arabidopsis thaliana. Plant Cell 20, 3300–3311.Google Scholar
Wang, ZJ, Huang, JQ, Huang, YJ, Zhang, B and Zheng, BS (2013) Study on pollination characteristics and hybrid offspring analysis on Hickory (Carya cathayensis), Advanced Materials Research 610, 3289–3297.Google Scholar
Wang, Y, Zhang, W, Song, L, Zou, J, Su, Z and Wu, W (2008b) Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis Plant Physiology 148, 1201–1211.Google Scholar
Weingartner, U, Kaeser, O, Long, M and Stamp, P (2002) Combining cytoplasmic male sterility and xenia increases grain yield of maize hybrids. Crop Science 42, 1848–1856.Google Scholar
Xiao, X, Li, H and Tang, C (2009) A silver-staining cDNA-AFLP protocol suitable for transcript profiling in the latex of Hevea brasiliensis (Para Rubber Tree). Molecular Biotechnology 42, 91–99.Google Scholar
Supplementary material: File

Pozzi et al. supplementary material

Table S1

Download Pozzi et al. supplementary material(File)
File 16.4 KB