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Changing topography of the RPE resulting from experimentally induced rapid eye growth

Published online by Cambridge University Press:  02 June 2009

Patricia A. Fleming ,
Alison M. Harman  and
Lyn D. Beazley
Patricia A. Fleming
Affiliation:
Department of Psychology, The University of Western Australia, Nedlands, Western Australia6009
Alison M. Harman
Affiliation:
Department of Psychology, The University of Western Australia, Nedlands, Western Australia6009
Lyn D. Beazley
Affiliation:
Deparlment of Zoology, The University of Western Australia, Nedlands, Western Australia6009
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Abstract

The retinal pigment epithelium (RPE) of the quokka wallaby, Setonix brachyurus, grows and changes throughout life. To investigate factors that determine changes in the quokka RPE, we have examined topography of this tissue in experimentally enlarged eyes. Unilateral eyelid suture was conducted at the time of normal eye opening, postnatal day (P) 110, and animals were examined at 1 or 1½ years of age. The numbers and densities of RPE cells and the extent of multinucleation were compared with those in normal animals. Eyelid suture resulted in a 9.8% and 17.4% increase in retinal area at 1 and 1½ years, respectively; a significant degree of myopia was associated with this enlargement. Cell density topography in experimental eyes was not the same as in controls. Cells from central retina were disproportionately larger in the experimental than control eyes. However, the RPE cell topography in sutured eyes was not the same as that of aged retinae of a similar size. Notably, in sutured eyes there was no development of the high or highest cell densities seen in equatorial and temporal central RPE in aged retinae, respectively. Furthermore, the degree of cell enlargement in peripheral regions was slight compared with that observed in similar-sized, aged retinae. There was no increase in RPE cell number; rather, average cell area increased accompanied by no change or a slight decrease in RPE thickness. Consequently, overall volume of cells did not change significantly. The large number of multinucleate cells normally seen in aged animals was not observed in experimentally enlarged eyes, implying that an increase in cell volume may be the trigger for multinucleation.

Keywords

AgingCell sizeMammalMultinucleateRetinal growthDevelopment
Type
Research Articles
Information
Visual Neuroscience , Volume 14 , Issue 3 , May 1997 , pp. 449 - 461
Copyright
Copyright © Cambridge University Press 1997

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References

REFERENCES

Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K. & Watson, J.D. (1989). Molecular Biology of the Cell (2nd edition). New York, London: Garland Publishing, Inc.Google Scholar
Beazley, L.D. & Dunlop, S.A. (1983). The evolution of an area centralis and visual streak in the marsupial Setonix brachyurus. Journal of Comparative Neurology 216, 211–231.CrossRefGoogle ScholarPubMed
Bell, F.C. & Stenstrom, W.J. (1983). Atlas of the Peripheral Retina. Sydney: W.B. Saunders company.Google Scholar
Bodenstein, L. & Sidman, R.L. (1987). Growth and development of the mouse RPE. I. Cell and tissue morphometrics and topography of mitotic activity. Developmental Biology 121, 192–204.CrossRefGoogle Scholar
Christensen, A.M. & Wallman, J. (1991). Evidence that increased scleral growth underlies visual deprivation myopia in chicks. Investigative Ophthalmology and Visual Science 32, 2143–2150.Google ScholarPubMed
Fankhauser, G. (1952). Nucleo-cytoplasmic relations in amphibian development. International Review of Cytology 1, 165–193.CrossRefGoogle Scholar
Fleming, P.A., Braekevelt, C.R., Harman, A.M. & Beazley, L.D. (1996 a). Retinal pigment epithelium and photoreceptor maturation in the quokka: A study of RPE cell generation and morphological development. Journal of Comparative Neurology (in press).3.0.CO;2-L>CrossRefGoogle Scholar
Fleming, P.A., Harman, A.M. & Beazley, L.D. (1996 b). Retinal pigment epithelium topography in the mature quokka, Setonix brachyurus. Experimental Eye Research 62. 85–93.CrossRefGoogle ScholarPubMed
Fleming, P.A., Harman, A.M. & Beazley, L.D. (1996 c). Development and aging of the RPE in a marsupial, the quokka. Experimental Eye Research 62, 457–469.CrossRefGoogle Scholar
Gottlieb, M.D., Joshi, H.B. & Nickla, D.L. (1991). Scleral changes in chicks with form deprivation myopia. Current Eye Research 237, 1157.Google Scholar
Gottlieb, M.D., Nickla, D.L. & Wallman, J. (1993). Evidence for a modulatory role of the choroid on the sclera in developing eyes. Investigative Ophthalmology and Visual Science (Supplement) 34, 1209.Google Scholar
Harman, A.M. (1991). Cell birth and death in the primary visual system of the wallaby, Setonix brachyurus (quokka). Journal of Comparative Neurology 313, 463–478.Google Scholar
Harman, A.M. & Beazley, L.D. (1986). Development of visual projections in the marsupial, Setonix brachurus. Anatomy of Embryology 175, 181–188.CrossRefGoogle Scholar
Harman, A.M. & Beazley, L.D. (1987). Patterns of cytogenesis in the developing retina of the wallaby, Setonix brachyurus. Anatomical Embryology 177, 123–130.CrossRefGoogle ScholarPubMed
Harman, A.M. & Beazley, L.D. (1989). Generation of retinal cells in the wallaby, Setonix brachyurus (quokka). Neuroscience 28, 219–232.CrossRefGoogle ScholarPubMed
Harman, A.M. & Jeffery, G. (1995). The development of the chiasm in a marsupial, the quokka wallaby. Journal of Comparative Neurology 359, 507–521.CrossRefGoogle Scholar
Harman, A.M., Eastough, N.J. & Beazley, L.D. (1995). The structure and development of the visual cortex of a wallaby—phylogenetic implications Brain, Behavior, and Evolution 45, 138–152.CrossRefGoogle Scholar
Harman, A.M., Snell, L.L. & Beazley, L.D. (1989). Cell death in the inner and outer nuclear layers of the developing retina in the wallaby Setonix brachyurus (quokka). Journal of Comparative Neurology 289, 1–10.CrossRefGoogle ScholarPubMed
Hodos, W., Fitzke, F.W., Hayes, B.P. & Holden, A.L. (1985). Experimental myopia in chicks: Ocular refraction by electroretinography. Investigative Ophthalmology and Visual Science 26, 1423–1430.Google ScholarPubMed
Hoyt, C.S., Stone, R.D., Fromer, C. & Billson, F.A. (1981). Monocular axial myopia associated with neonatal eyelid closure in human infants. American Journal of Ophthalmology 91, 197–200.CrossRefGoogle ScholarPubMed
Hughes, A. (1977). The topography of vision in mammals. In Handbook of Sensory Physiology: The Visual System in Vertebrates, ed. Crescitelli, F., pp. 615–756. Berlin: Springer-Verlag.Google Scholar
Kang, R.N. & Norton, T.T. (1993). Alteration of scleral morphology in tree shrews with induced myopia. Investigative Ophthalmology and Visual Science 34, 1209.Google Scholar
Kelling, S.T., Sengelaub, D.R., Wikler, K.C. & Finlay, B.L. (1989). Differential elasticity of the immature retina: A contribution to the development of the area centralis? Visual Neuroscience 2, 117–120.CrossRefGoogle Scholar
Kirchhof, B., Kirchhof, E., Ryan, S.J. & Sorgente, N. (1989). Vitreous modulation of migration and proliferation of RPE cells in vitro. Investigative Ophthalmology and Visual Science 30, 1951–1957.Google Scholar
Lin, T., Grimes, P.A. & Stone, R.A. (1993). Expansion of the retinal pigment epithelium in experimental myopia. Vision Research 33, 1881–1885.CrossRefGoogle ScholarPubMed
Lodge, A., Peto, T. & Mcfadden, S.A. (1994). Form deprivation myopia and emmetropization in the guinea pig. In Proceedings of the Australian Neuroscience Society 5, 123.Google Scholar
Marshak, T.L., Stroeva, O.G. & Brodsky, V.Y. (1976). Specialisation of uni- and binucleated cells of the retinal pigment epithelium during early and postnatal development in the rat. Zhural Obschei Biologii 37, 608–614.Google Scholar
Mastronarde, D.N., Thibeault, M.A. & Dubin, M.W. (1984). Nonuniform postnatal growth of the cat retina. Journal of Comparative Neurology 228, 598–608.CrossRefGoogle ScholarPubMed
Mcbrien, N.A. & Norton, T.T. (1992). The development of experimental myopia and ocular component dimensions in monocularly lid-sutured tree shrews (Tupaia belangeri) Vision Research 32, 843–852.CrossRefGoogle ScholarPubMed
O'leary, D.J. & Millodot, M. (1979). Eyelid closure causes myopia in humans. Experientia 35, 1478–1479.CrossRefGoogle ScholarPubMed
Rabin, J., Van Sluyters, R. & Malach, R. (1981). Emmetropization: A vision-dependent phenomenon. Investigative Ophthalmology and Visual Science 20, 561–564.Google ScholarPubMed
Rada, J.A., Wallman, J. & Hassell, J.R. (1993). Increased corneal growth in a chicken model of myopia. Investigative Ophthalmology and Visual Science 34, 1208.Google Scholar
Robb, R.M. (1977). Refractive errors associated with hemangiomas of the eyelids and orbit in infancy. American Journal of Ophthalmology 83, 52–58.CrossRefGoogle ScholarPubMed
Robinson, S.R. (1991). Development of the mammalian retina. In Neuroanatomy of the Visual Pathways and Their Development, ed. Dreher, B. & Robinson, S.R. pp. 69–128. UK: McMillan.Google Scholar
Stroeva, O.G. & Panova, I.G. (1983). Retinal pigment epithelium: Pattern of proliferative activity and its regulation by intraocular pressure in postnatal rats. Journal of Embryology and Experimental Morphology 75, 271–291.Google ScholarPubMed
Swanson, C.P. (1969). The Cell (3rd edition). New Jersey: Prentice-Hall, Inc.Google Scholar
Teakle, E.M., Wildsoet, C.F. & Vaney, D.I. (1993). The spatial organization of tyrosine hydroxylase immunoreactive amacrine cells in the chicken retina and the consequences of myopia. Vision Research 33, 2383–2396.CrossRefGoogle ScholarPubMed
Troilo, D. (1990). Experimental studies of emmetropization in the chick. In Myopia and the Control of Eye Growth, Ciba Foundation Symposium 155, pp. 89–114. Chichester: Wiley.Google Scholar
Troilo, D. & Judge, S.J. (1993). Ocular development and visual deprivation myopia in the common marmoset (Callithrix jacchus) Vision Research 33, 1311–1324.CrossRefGoogle ScholarPubMed
Wallman, J., Turkel, J. & Trachtman, J. (1978). Extreme myopia produced by modest change in early visual experience. Science 201, 1249–1251.CrossRefGoogle ScholarPubMed
Walls, G.L. (1942). The Vertebrate Eye and Its Adapiive Radiation. Bloomfield Hills, Michigan: Cranbrook Institute of Science Press.Google Scholar
Wiesel, T.N. & Raviola, E. (1977). Myopia and eye enlargement after neonatal lid fusion in monkeys. Nature 266, 66–67.CrossRefGoogle ScholarPubMed
Wildsoet, C.F. & Pettigrew, J.D. (1988). Kainic acid-induced eye enlargement in chickens: Differential effects on anterior and posterior segments. Investigative Ophthalmology and Visual Science 29, 311–319.Google ScholarPubMed
Wilson, J.R. & Sherman, S.M. (1977). Differential effects of early monocular deprivation on binocular and monocular segments of cat striate cortex. Journal of Neurophysiology 40, 891–903.CrossRefGoogle ScholarPubMed
Yinon, U., Rose, L. & Shapiro, A. (1980). Myopia in the eye of developing chicks following monocular and binocular lid closure. Vision Research 20, 137–141.CrossRefGoogle ScholarPubMed