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Localization of GABAA receptor subtypes in the tiger salamander retina

Published online by Cambridge University Press:  02 June 2009

Chen-YU Yang ,
Zhen-Shi Lin  and
Stephen Yazulla
Chen-YU Yang
Affiliation:
Department of Neurobiology and Behavior, State University of New York, Stony Brook
Zhen-Shi Lin
Affiliation:
Department of Neurobiology and Behavior, State University of New York, Stony Brook
Stephen Yazulla
Affiliation:
Department of Neurobiology and Behavior, State University of New York, Stony Brook
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Abstract

Dry autoradiography was used to determine the distribution of GABAA binding sites in tiger salamander retina. High-affinity binding of [3H]-flunitrazepam ([3H]-FNZ) was used to localize benzodiazepine receptors (BZR) and [3H]-muscimol was used to localize the GABAA recognition site. Specific [3H]-FNZ binding was present only in the inner retina, primarily in the inner plexiform layer (IPL). Co-incubation with GABA enhanced [3H]-FNZ binding by 20–50%. [3H]-muscimol binding was found throughout the IPL and in the outer plexiform layer (OPL). Mouse monoclonal antibodies 62–3G1 and BD-17, that recognize the GABAAβ2, β3 polypeptides, and BD-24, that recognizes the GABAA α1, polypeptide, did not label either the OPL or IPL, despite numerous variations in the fixation and immunoprocessing methods. GABAA receptor location, as revealed by [3H]-muscimol binding, matches the distribution of presumed GABAergic terminals in the OPL and IPL. We suggest that there are at least two subtypes of GABAA receptor in the tiger salamander retina: one type is present only in the inner retina, primarily in the IPL and is functionally coupled to BZRs; the other type is located in the OPL and is not coupled to the BZRs. Furthermore, GABAA receptors in the tiger salamander retina appear to be of a different epitope than GABAA receptors in numerous other preparations that are recognized by mAbs 62–3G1, BD-17, and BD-24.

Keywords

GABAA receptorBenzodiazepine receptorDry autoradiographyTiger salamander retina
Type
Research Articles
Information
Visual Neuroscience , Volume 8 , Issue 1 , January 1992 , pp. 57 - 64
Copyright
Copyright © Cambridge University Press 1992

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