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Echinococcus shiquicus in Qinghai–Tibet plateau: population structure and confirmation of additional endemic areas

Published online by Cambridge University Press:  24 March 2021

Hong-Bin Yan ,
Li Li ,
Wenhui Li ,
Guoqiang Zhu ,
Jian-Qiu Li ,
Yantao Wu ,
Nianzhang Zhang ,
Yaodong Wu ,
Min Li  and
Linsheng Zhang
...Show all authors
Hong-Bin Yan*
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Li Li
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Wenhui Li
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Guoqiang Zhu
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Jian-Qiu Li
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China Linyi Vocational University of Science and Technology, Linyi276000, Shandong Province, People's Republic of China
Yantao Wu
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Nianzhang Zhang
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Yaodong Wu
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Min Li
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Linsheng Zhang
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Gang Yao
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Wenjun Tian
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Le Li
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Wenjing Li
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Aimin Guo
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Guodong Dai
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Baoquan Fu
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease, Yangzhou225009, Jiangsu Province, People's Republic of China
John Asekhaen Ohiolei*
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China
Wan-Zhong Jia*
Affiliation:
State Key Laboratory of Veterinary Etiological Biology/National Professional Laboratory for Animal Echinococcosis/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou730046, Gansu Province, People's Republic of China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease, Yangzhou225009, Jiangsu Province, People's Republic of China
*
Authors for correspondence: Wan-Zhong Jia, E-mail: jiawanzhong@caas.cn; Hong-Bin Yan, yanhongbin@caas.cn; John Asekhaen Ohiolei, asekhaenj@gmail.com
Authors for correspondence: Wan-Zhong Jia, E-mail: jiawanzhong@caas.cn; Hong-Bin Yan, yanhongbin@caas.cn; John Asekhaen Ohiolei, asekhaenj@gmail.com
Authors for correspondence: Wan-Zhong Jia, E-mail: jiawanzhong@caas.cn; Hong-Bin Yan, yanhongbin@caas.cn; John Asekhaen Ohiolei, asekhaenj@gmail.com
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Abstract

Echinococcus shiquicus is currently limited to the Qinghai–Tibet plateau, a large mountainous region in China. Although the zoonotic potential remains unknown, progress is being made on the distribution and intermediate host range. In this study, we report E. shiquicus within Gansu and Qinghai provinces in regions located not only around the central areas but also the southeast edge of the plateau and describe their genetic relationship with previous isolates from the plateau. From 1879 plateau pikas examined, 2.39% (95% CI 1.79–3.18) were infected with E. shiquicus. The highest prevalence of 10.26% (4.06–23.58) was recorded in Makehe town, Qinghai province. Overall the prevalence was marginally higher in Qinghai (2.5%, CI 1.82–3.43) than in Gansu (2%, CI 1.02–3.89). The cox1 and nad1 genes demonstrated high and low haplotype and nucleotide diversities, respectively. The median-joining network constructed by the cox1–nad1 gene sequences demonstrated a star-like configuration with a median vector (unsampled haplotype) occupying the centre of the network. No peculiar distinction or common haplotype was observed in isolates originating from the different provinces. The presence of E. shiquicus in regions of the southeast and northeast edges of the Qinghai–Tibet plateau and high genetic variation warrants more investigation into the haplotype distribution and genetic polymorphism by exploring more informative DNA regions of the mitochondrial genome to provide epidemiologically useful insight into the population structure of E. shiquicus across the plateau and its axis.

Keywords

Echinococcus shiquicusgenetic variationhaplotypespikasQinghai–Tibet plateauvoles
Type
Research Article
Information
Parasitology , Volume 148 , Issue 7 , June 2021 , pp. 879 - 886
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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