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Molecular characterization and expression analysis of three novel autophagy-related genes from the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

Published online by Cambridge University Press:  09 September 2016

JOSÉ MIGUEL FLORES FERNÁNDEZ ,
CARLA PATRICIA BARRAGÁN ÁLVAREZ ,
CARLA VANESSA SÁNCHEZ HERNÁNDEZ ,
EDUARDO PADILLA CAMBEROS ,
CELIA GONZÁLEZ CASTILLO ,
DANIEL ORTUÑO SAHAGÚN  and
MOISÉS MARTÍNEZ VELÁZQUEZ
JOSÉ MIGUEL FLORES FERNÁNDEZ
Affiliation:
Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270. Guadalajara, Jalisco, México
CARLA PATRICIA BARRAGÁN ÁLVAREZ
Affiliation:
Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270. Guadalajara, Jalisco, México
CARLA VANESSA SÁNCHEZ HERNÁNDEZ
Affiliation:
Departamento de Producción Agrícola, CUCBA, Universidad de Guadalajara, Carretera Guadalajara-Nogales km 15.5, 45110, Zapopan, Jalisco, México
EDUARDO PADILLA CAMBEROS
Affiliation:
Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270. Guadalajara, Jalisco, México
CELIA GONZÁLEZ CASTILLO
Affiliation:
Instituto de Investigación en Ciencias Biomédicas (IICB), CUCS, Universidad de Guadalajara, Sierra Mojada 950, Col. Independencia, 44340. Guadalajara, Jalisco, México
DANIEL ORTUÑO SAHAGÚN
Affiliation:
Instituto de Investigación en Ciencias Biomédicas (IICB), CUCS, Universidad de Guadalajara, Sierra Mojada 950, Col. Independencia, 44340. Guadalajara, Jalisco, México
MOISÉS MARTÍNEZ VELÁZQUEZ*
Affiliation:
Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270. Guadalajara, Jalisco, México
*
*Corresponding author: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Col. Colinas de la Normal, 44270. Guadalajara, Jalisco, México. E-mail: moisesmartinezv@yahoo.com.mx
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Summary

The cattle tick Rhipicephalus (Boophilus) microplus is a hematophagous ectoparasite of major importance for the livestock industry. It shows a remarkable ability to survive over long periods without feeding. However, the mechanisms used to endure long-term starvation are poorly understood. It is believed that autophagy, a process of intracellular protein degradation, may play a significant role to confront adverse environmental conditions. To advance our understanding of autophagy in R. microplus, in the present study we report the molecular characterization of three autophagy-related (ATG) genes, namely, RmATG3, RmATG4 and RmATG6, as well as their expression profiles in different developmental stages and organs of the parasite. The deduced amino acid sequences derived from the characterized gene sequences were subjected to Basic Local Alignment Search Tool analysis. The testing produced significant alignments with respective ATG proteins from Haemaphysalis longicornis and Ixodes scapularis ticks. Real-time polymerase chain reaction assays revealed that RmATG4 and RmATG6 transcripts were elevated in egg and ovary tissue, when compared with larva and midgut samples, while RmATG3 expression in midgut was 2-fold higher than in egg, larva and ovary samples.

Keywords

AutophagyATG3ATG4ATG6Rhipicephalus microplusreal-time PCRdevelopmentembryogenesis
Type
Research Article
Information
Parasitology , Volume 143 , Issue 13 , November 2016 , pp. 1802 - 1809
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Copyright
Copyright © Cambridge University Press 2016 

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