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Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus

Published online by Cambridge University Press:  06 April 2009

H. D. F. H. Schallig ,
M. A. W. van Leeuwen  and
W. M. L. Hendrikx
H. D. F. H. Schallig
Affiliation:
Utrecht University, Institute of Infectious Diseases and Immunology, Department of Parasitology and Tropical Veterinary Medicine, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands
M. A. W. van Leeuwen
Affiliation:
Utrecht University, Institute of Infectious Diseases and Immunology, Department of Parasitology and Tropical Veterinary Medicine, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands
W. M. L. Hendrikx
Affiliation:
Utrecht University, Institute of Infectious Diseases and Immunology, Department of Parasitology and Tropical Veterinary Medicine, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands
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Summary

The excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgGI levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material- based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections.

Keywords

Haemonchus contortusexcretory/secretory productsTexel sheepimmune response
Type
Research Article
Information
Parasitology , Volume 108 , Issue 3 , April 1994 , pp. 351 - 357
Copyright
Copyright © Cambridge University Press 1994

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