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The detection of AKH/HrTH-like peptides in Ascaridia galli and Ascaris suum using an insect hyperglycaemic bioassay

Published online by Cambridge University Press:  06 April 2009

T. R. B. Davenport ,
L. A. Eaves ,
T. K. Hayes ,
D. L. Lee  and
R. E. Isaac
T. R. B. Davenport
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT, UK
L. A. Eaves
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT, UK
T. K. Hayes
Affiliation:
Department of Entomology, Texas A&M University, College Station, TX 77843-2475, USA
D. L. Lee
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT, UK
R. E. Isaac
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT, UK
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Summary

Evidence for the presence of adipokinetic hormone/hypertrehalosaemic hormone (AKH/HrTH)-like peptides in the parasitic nematodes Ascaridia galli and Ascaris suum has been obtained using insect bioassays which measure hyperglycaemic responses to peptides belonging to the AKH/HrTH family of insect hormones. A peptide fraction extracted from heads and tails of Ascaridia galli evoked a dose-dependent hyperglycaemic response when injected into the cockroach, Periplaneta americana. Maximal bioactivity was obtained with material that was equivalent to 38 mg (wet weight) of nematode. Bioactivity appeared to be highest in extracts from heads and tails of both male and female worms and could be fractionated into at least three peaks of hyperglycaemic activity by reversed-phase high-performance liquid chromatography. An extract from heads and tails of A. suum also evoked a hyperglycaemic response when injected into the cockroach, Blaberus discoidalis. The bioactivity was inactivated on incubation with pure endopeptidase 24.11, confirming the peptidic nature of the bioactive material. These results provide evidence for the existence of peptides related to the insect AKH/HrTH family of peptides in parasitic nematodes.

Keywords

Ascaridia galliAscaris suumnematodesadipokinetic hormone/hypertrehalosaemic hormone
Type
Research Article
Information
Parasitology , Volume 108 , Issue 4 , May 1994 , pp. 479 - 485
Copyright
Copyright © Cambridge University Press 1994

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