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Molecular typing of Staphylococcus aureus isolated from Italian dairy products on the basis of coagulase gene polymorphism, multiple-locus variable-number tandem-repeat and toxin genes

Published online by Cambridge University Press:  14 August 2008

Morandi Stefano ,
Brasca Milena ,
Lodi Roberta  and
Brusetti Lorenzo
Morandi Stefano*
Affiliation:
Institute of Sciences of Food Production, Italian National Research Council, via Celoria 2, 20133 Milan, Italy
Brasca Milena
Affiliation:
Institute of Sciences of Food Production, Italian National Research Council, via Celoria 2, 20133 Milan, Italy
Lodi Roberta
Affiliation:
Institute of Sciences of Food Production, Italian National Research Council, via Celoria 2, 20133 Milan, Italy
Brusetti Lorenzo
Affiliation:
Department of Food Science and Microbiology (DISTAM), Università degli Studi di Milano, via Celoria 2, 20133, Milan, Italy
*
*For correspondence; e-mail: stefano.morandi@ispa.cnr.it
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Abstract

Coagulase gene restriction fragment length polymorphism (RFLP), six-locus variable-number tandem-repeat analysis patterns (MLVA) and detection of enterotoxin genes (se) (sea, sec, sed, seg, seh, sei, sej and sel) were used to determine the phylogenetic relationship among isolates of Staphylococcus aureus isolated from dairy products from different regions of Italy. A total of 25 Staph. aureus were subtyped into 16 coagulase genotypes by RFLP, and MLVA revealed marked genomic variability. Furthermore, 17 of the isolates harboured at least one toxin gene, with the predominance of sea, sed and sej among cow isolates and sec-sel among the goat and sheep strains. Combined RFLP, MLVA polymorphism and se genes were found to be useful techniques for discriminating several genetic variants in Staph. aureus isolates.

Keywords

Staphylococcus aureusenterotoxinscoagulasemolecular typingMLVARFLP
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 4 , November 2008 , pp. 444 - 449
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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