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The distribution of carboxymethylcellulase between fractions taken from the rumens of sheep containing no protozoa or one of five different protozoal populations

Published online by Cambridge University Press:  27 March 2009

G. S. Coleman
G. S. Coleman
Affiliation:
Biochemistry Department, Agricultural and Food Research Council, Institute of Animal Physiology, Babraham, Cambridge, CB2 4AT
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Summary

The distribution of carboxymethylcellulase (CMCase) between various rumen fractions has been determined at three times after feeding in sheep containing no ciliate protozoa or five different protozoal populations. The total rumen CMCase was lowest in defaunated animals and tended to be higher in animals containing the amylolytic Entodinium caudatum or a natural mixed protozoal population and highest in sheep containing single cellulolytic species. In animals containing the cellulolytic species, Epidinium ecaudatum caudatum or Eudiplodinium maggii, 70% of the CMCase was associated with the protozoal fraction whereas less than 15% was present in a fraction prepared in the same way from sheep containing no protozoa or only Entodinium caudatum. The activity associated with the free bacteria was higher in sheep containing only Entodinium caudatum than in defaunated animals or those containing the cellulolytic species. Similar results were obtained 1·5, 6·5 or 24 h after feeding. Almost no activity was present in the cell-free rumen liquor under any condition. A variable amount of CMCase (up to 25% of the total) was associated with the plant debris in the rumen and the specific activity of the enzyme released by sonication was often higherthan that released from the free bacteria under the same conditions.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 106 , Issue 1 , February 1986 , pp. 121 - 127
Copyright
Copyright © Cambridge University Press 1986

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References

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