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Potential for hitchhiking in the eda-edd-zwf gene cluster of Escherichia coli

Published online by Cambridge University Press:  14 April 2009

Daniel E. Dykhuizen ,
Jean De Framond  and
Daniel L. Hartl
Daniel E. Dykhuizen
Affiliation:
Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110
Jean De Framond
Affiliation:
Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110
Daniel L. Hartl
Affiliation:
Department of Genetics, Washington University School of Medicine, St Louis, Missouri 63110
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The loci eda, edd and zwf form a tightly linked cluster in E. coli that functions in the metabolism of galacturonate, gluconate and glucose. This cluster has been transferred from six natural isolates into the genetic background of E. coli K12 and examined with regard to effects on growth rate in chemostats. Although the naturally occurring eda and zwf alleles are selectively neutral, the edd alleles are not. The edd alleles fall into three functional classes distinguished by their effects on growth rate in gluconate medium, the most common classes differing in fitness by approximately 1% per hour. This extensive non-neutral genetic variation of edd is discussed in light of the evident rarity of gluconate as a natural substrate. We propose that gluconate selection is intermittent in space or time, providing the population an opportunity to accumulate non-neutral genetic variants during periods of relaxed selection. Such genetic variants will eventually be sorted out by the intermittent periods of gluconate selection, and during these periods the linked eda and zwf alleles will experience pronounced hitchhiking effects.

Type
Research Article
Information
Genetics Research , Volume 43 , Issue 3 , June 1984 , pp. 229 - 239
Copyright
Copyright © Cambridge University Press 1984

References

REFERENCES

Bachmann, B. (1983). Linkage map of Escherichia coli K-12, 7th ed.Microbiological Reviews 47, 180–230.Google Scholar
Crow, J. F. & Kimura, M. (1970). An Introduction to Population Genetics Theory. New York: Harper & Row.Google Scholar
Dykhuizen, D. E. & Hartl, D. L. (1980). Selective neutrality of 6PGD allozymes in E. coli and the effects of genetic background. Genetics 96, 801–817.CrossRefGoogle Scholar
Dykhuizen, D. E. & Hartl, D. L. (1983 a). Functional effects of PGI allozymes in Escherichia coli. Genetics 105, 1–18.CrossRefGoogle ScholarPubMed
Dykhuizen, D. E. & Hartl, D. L. (1983 b). Selection in chemostats. Microbiological Reviews 47, 150–168.CrossRefGoogle ScholarPubMed
Dykhuizen, D. E., De Framond, J. & Hartl, D. L. (1984). Selective neutrality of glucose-6-phosphate dehydrogenase allozymes in Escherichia coli. Molecular Biology and Evolution 1, 162–170.Google ScholarPubMed
Fraenkel, D. G. (1968). Selection of Escherichia coli mutants lacking glucose-6-phosphate dehydrogenase or gluconate-6-phosphate dehydrogenase. Journal of Bacteriology 95, 1267–1271.CrossRefGoogle ScholarPubMed
Hartl, D. L. & Dykhuizen, D. E. (1981). Potential for selection among nearly neutral allozymes of 6-phosphogluconate dehydrogenase in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 78, 6344–6348.CrossRefGoogle ScholarPubMed
Kacser, H. & Burns, J. A. (1973). The control of fiux. Symposium of the Society for Experimental Biology 27, 65–104.Google Scholar
Kacser, H. & Burns, J. A. (1979). Molecular democracy: who shares the controls? Biochemical Society Transactions 7, 1149–1160.CrossRefGoogle ScholarPubMed
Mason, T. G. & Richardson, G. (1981). A review: Escherichia coli and the human gut: some ecological considerations. Journal of Applied Bacteriology 1, 1–16.CrossRefGoogle Scholar
Milkman, R. (1973). Electrophoretic variation in Escherichia coli from natural sources. Science 182, 1024–1026.CrossRefGoogle ScholarPubMed
Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.Google Scholar
Ritzenthaler, P., Blanco, C. & Mata-Gilsinoer, M. (1983). Interchangeability of repressore for the control of the uxu and uid operons in E. coli K12. Molecular and General Genetics 191, 263–270.CrossRefGoogle Scholar
Schaffer, R. (1972). Occurrence, properties and preparation of naturally occurring monosac-charides (including 6-deoxy sugars). In The Carbohydrates, vol. 1 A (ed. Pigman, W. and Horton, D.), pp. 69–111. New York: Academic Press.CrossRefGoogle Scholar
Selander, R. K. & Levin, B. R. (1980). Genetic diversity and structure in Escherichia coli populations. Science 210, 545–547.CrossRefGoogle ScholarPubMed
Shallenberger, R. S. (1974). Occurrence of various sugars in foods. In Sugars in Nutrition (ed. Sipple, H. L. and McNutt, K. W.), pp. 67–80. New York: Academic Press.Google Scholar
Snedecor, G. W. & Cochhan, W. G. (1967). Statistical Methods, 6th ed.Ames, Iowa: Iowa State University Press.Google Scholar