Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-24T13:50:52.420Z Has data issue: false hasContentIssue false
  • English
  • Français

Intracellular growth of Legionella pneumophila serogroup 1 monoclonal antibody type 2 positive and negative bacteria

Published online by Cambridge University Press:  15 May 2009

P. H. Edelstein  and
M. A. C. Edelstein
P. H. Edelstein
Affiliation:
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104–4283 Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104–4283
M. A. C. Edelstein
Affiliation:
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104–4283
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Epidemiological evidence suggests that monoclonal antibody type 2 positive (MAB 2+) Legionella pneumophila serogroup 1 (LP1) more often causes disease than do MAB 2− isolates, and there is evidence that MAB 2− LP1 grow less well in cells than do MAB 2+ bacteria. We tested the intracellular growth rates of ten randomly selected MAB 2− LP1 isolates, by using guinea-pig alveolar macrophages, and human monocyte-derived macrophages. Save a low virulence control, all ten MAB 2− isolates grew as well in cells as a virulent MAB 2+ isolate. Heterogeneity of MAB 2− LP1 growth in cells exists, making poor intracellular growth an unlikely explanation for why MAB 2+ LP1 appear to cause disease more often.

Type
Research Article
Information
Epidemiology & Infection , Volume 111 , Issue 3 , December 1993 , pp. 499 - 502
Copyright
Copyright © Cambridge University Press 1993

References

1.Joly, JR, McKinney, RM, Tobin, JO, Bibb, WF, Watkins, ID. Ramsay, D. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol 1986; 23: 768–71.CrossRefGoogle ScholarPubMed
2.Brindle, RJ, Stannett, PJ, Cunliffe, RN. Legionella pneumophila: comparison of isolation from water specimens by centrifugation and filtration. Epidemiol Infect 1987: 99: 241–7.CrossRefGoogle Scholar
3.Dournon, E, Bibb, WF, Rajagopalan, P, Desplaces, N. McKinney, RM. Monoclonal antibody reactivity as a virulence marker for Legionella pneumophila serogroup 1 strains. J Infect Dis 1988; 157: 496–501.CrossRefGoogle ScholarPubMed
4.Joly, JR, Winn, WC. Correlation of subtypes of Legionella pneumophila defined by monoclonal antibodies with epidemiological classification of cases and environmental sources. J Infect Dis 1984; 150: 667–71.CrossRefGoogle ScholarPubMed
5.Plouffe, JF, Para, MF, Maher, WE, Hackman, B, Webster, L. Subtypes of Legionella pneumophila serogroup 1 associated with different attack rates. Lancet 1983; ii: 649–50.CrossRefGoogle Scholar
6.Bollin, GE, Plouffe, JF, Para, MF. Prior, RB. Difference in virulence of environmental isolates of Legionella pneumophila. J Clin Microbiol 1985; 21: 674–7.CrossRefGoogle ScholarPubMed
7.Dennis, PJ, Lee, JV. Differences in aerosol survival between pathogenic and non-pathogenic strains of Legionella pneumophila serogroup 1. J Appl Bacteriol 1988; 65: 135–41.CrossRefGoogle ScholarPubMed
8.Dournon, E, Rajagopalan, P. Correlation between monoclonal antibody pattern. serum sensitivity and intracellular growth of L. pneumophila. Abstr 87th Annu Meet Am Soc Microbiol 1987; Abst. B-279. p. 71.Google Scholar
9.Molina, JM, Rajagopalan, P, Dournon, E. Monoclonal antibody pattern of L. pneumophila associated with virulence in guinea pigs. Abstr 87th Annu Meet Am Soc Microbiol 1987: Abst. B-278, p. 71.Google Scholar
10.Edelstein, PH, Beer, KB, DeBoynton, ED. Influence of growth temperature on virulence of Legionella pneumophila. Infect Immun 1987; 55: 2701–5.CrossRefGoogle ScholarPubMed
11.Edelstein, PH. Legionnaires' disease laboratory manual. 3rd ed. Springfield. VA: National Technical Information Service, 1985.Google Scholar
12.Edelstein, PH, Nakahama, C, Tobin, JO, et al. Paleoepidemiologic investigation of Legionnaires' disease at Wadsworth Veterans Administration Hospital by using three typing methods for comparison of legionellae from clinical and environmental sources. J Clin Microbiol 1986; 23: 1121–6.CrossRefGoogle ScholarPubMed
13.Edelstein, PH. Detection of antibodies to Legionella. In: Rose, NR. de Macario, EC. Fahey, JL, Friedman, H, Penn, GM, eds. Manual of clinical laboratory immunology. 4th ed. Washington, D.C.: American Society for Microbiology, 1992: 459–66.Google Scholar
14.Hassan, NF, Cutilli, JR. Douglas, SD. Isolation of highly purified human blood monocytes for in vitro HIV-1 infection studies of monocyte/macrophages. J Immunol Methods 1990: 130: 283–5.CrossRefGoogle ScholarPubMed
15.Horwitz, MA, Silverstein, SC. Legionnaires' disease bacterium (Legionella pneumophila) multiplies intracellularly in human monocytes. J Clin Invest 1980; 73: 441–50.CrossRefGoogle Scholar
16.Rogers, JE, Eisenstein, BI, Engleberg, NC. Spontaneous changes in LPS and monoclonal antibody binding in a single line of L. pneumophila serogroup 1 cells. 1992 Intl Symp Legionella 1992: Abstr i–7. p. 13.Google Scholar